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Citations for BioLegend :
151 - 200 of 6674 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection using MojoSort Human CD4 T Cell Isolation kit (Biolegend). Primary CD4+ T cells were maintained in complete RPMI 1640 medium supplemented with 10 IU/ml IL-2 (Center for AIDS Reagents [CFAR] ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were isolated by negative-selection using MOJOSORT™ Human CD4 T Cell Isolation Kit (Biolegend) and a EASYSEP™ Magnet (StemCell ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were separated from PBMCs using a MojoSort human CD3+ T cell isolation kit (Biolegend, USA).
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by incubating cells with fluorescein isothiocyanate-conjugated anti-human CD8 (Biolegend, 100705) and phycoerythrin-conjugated anti-human CD25 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were stained with PE-labelled anti-human CD155 (BioLegend, 337610, 1:300), corresponding isotype control antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... memory cells were purified after treatment with mouse anti-human CD45RA mAbs (Biolegend) and Goat anti-Mouse IgG covered Dynabeads (Invitrogen).
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bioRxiv - Immunology 2019Quote: ... Cell surface staining was performed with mouse anti-human CD45 (clone HI30; BioLegend), CD3 (clone SP34-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Approximately 1 million cells were resuspended in PBS containing human TruStain FcX (Biolegend) and incubated at 4C for 10 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with APC/Cy7 anti-human CD4 (clone RPA-T4, Biolegend) and AlexaFluor647 anti-mouse H-2Kd (clone SF1-1.1 ...
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bioRxiv - Cell Biology 2021Quote: Cell surface staining was performed with human CD19-PE (clone HIB 19; BioLegend) antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated with an APC-conjugated anti-human VEGFR3 (FLT4) antibody (BioLegend) for 20 minutes at 4 °C and were then subjected to two iterative rounds of washing (and centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then treated with 100 ng/ml of recombinant human IFNγ (Biolegend) for 24h ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were harvested and incubated with APC anti-human CD184 (CXCR4) [12G5] (BioLegend) for 1 hr and then fluorescence was analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... B cells were then stained with FITC anti-human CD19 antibody (BioLegend, 392508), FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... mononuclear cells were stained with anti-human CD34 BV650 (Biolegend, Cat# 343624, RRID:AB_2632725) for 20min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The single-cell suspension was resuspended in Fc-Block (Human TruStain FcX, BioLegend) and incubated with CD31 microbeads (Miltenyi Biosciences ...
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bioRxiv - Cell Biology 2022Quote: Lung and fibroblast single-cell suspensions were incubated with human TruStain FcX (Biolegend) for 30 min on ice to block Fc receptors ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated with Fc Receptor Blocking Solution (Human TruStain FcX, Biolegend) according to the manufacturer’s instructions for 10 minutes ...
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bioRxiv - Immunology 2019Quote: ... cells were intracellularly stained with anti-human IFN-γ-PECy7 (clone B27, Biolegend). Flow cytometric analysis was performed using the FACS LSR Fortessa X20 (BD Bioscience) ...
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bioRxiv - Cancer Biology 2019Quote: ... Single cells that bound an APC-conjugated anti-human CD326 antibody (324208, BioLegend) and did not bind to a FITC-conjugated anti-mouse H-2Kd antibody (116606 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated with biotin-anti-human CD274/PD-L1 (BioLegend) on ice for 20 min ...
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bioRxiv - Immunology 2020Quote: ... and Human Hematopoietic Stem Cell Panels for M-CSF and GM-CSF (BioLegend). Flow cytometry data were acquired on a Cytoflex LX (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with 10 microliters of Human TruStain FcX blocking solution (BioLegend) for 10 minutes at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... Human colon cells were stained with PB-conjugated antiCD45 (#304022, BioLegend; 1:400), PB-conjugated anti-CD31 (#303114 ...
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bioRxiv - Microbiology 2022Quote: ... Cell staining was done using an APC-conjugated anti-human CD11b (#101211, Biolegend), PE-conjugated anti-human CD11c (#333149 ...
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bioRxiv - Microbiology 2022Quote: ... and sCD40L were measured using the human B cell premix kit from Biolegend Legendplex™ using manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 50,000 cells were used and blocked with Human TruStain FcX (Fc block, BioLegend) in FACS buffer (0.5% BSA (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Single cell suspension was stained with PE anti-human CD82 Antibody (BioLegend, 342103) in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Following fixation cells were stained with FITC anti-mouse/human Ki-67 (BioLegend) and DAPI ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions were blocked with either Human TruStain FcX Solution (422301, Biolegend) or mouse TruStain FcX PLUS (anti-mouse CD16/32 ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained with 1:40 FITC anti-human CD45 antibody (304006, Biolegend) and 1:400 LIVE/DEAD™ Fixable Near-IR Dead Cell Stain (L10119 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 25 min with biotinylated anti-human MMR (321118, BioLegend), washed ...
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bioRxiv - Neuroscience 2023Quote: ... 1x105 cells were incubated with Human TruStain FcX (Fc Receptor Blocking Solution) (Biolegend) for 30 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were blocked with 2.5% Human TruStain FcX blocking solution (BioLegend; 422302) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with APC/Cyanine7 anti-human CD14 (clone: 63D3 BioLegend®) and Brilliant Violet 421™ anti-human CD209 (clone ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell suspensions were then stained using the directly-labelled primary antibody TNFα_PE (BioLegend; 502929) or mouse IgG1 (eBiosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated overnight with primary antibodies Nestin (Biolegend, Cat# 839801, 1:200), MAP-2 (Santa Cruz ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... Primary cells were stained for purity post-isolation using anti-CD3 (OKT3, BioLegend, # 317301), and Near IR Live/dead stain (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Neuroscience 2023Quote: ... leptomeninges and brain cells were pelleted at 300xg for 10 minutes and incubated with anti-CD11b APC/Cy7 (BioLegend cat. #101226) and anti-CD45 BV510 (BD Biosciences cat ...