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Citations for BioLegend :
201 - 250 of 2412 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the pellets were resuspended and incubated for 15 min on ice in 0.5 ml MEM containing CD24-APC antibody (1:500, 101814, Biolegend, San Diego, CA). After centrifugation for 5 min at 200 × g at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 0.5 tests of anti-CD4 (Biolegend 344651) hashed antibody and 0.5 tests of anti-CD8 hashed antibody (Biolegend 344753 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD38-APC (Biolegend, clone HIT2, 0.5:50) and HLA-DR BV605 (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL, VCAM-1, 429 MVCAM.A, BioLegend) were used ...
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bioRxiv - Biophysics 2023Quote: ... and 6.25 μg/mL activation antibodies against Integrin β2 (clone m24 and LFA1/2, Biolegend) were added in cell culture medium at the start of the culture.
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes and peritoneal cells were resuspended in staining buffer (PBS containing 2% newborn calf serum) and pre-incubated with 0.5 μg/ml FcBlock (eBioscience) and stained with fluorochrome-conjugated mAbs (Biolegend, eBioscience, and BD Biosciences): CD19 (1D3) ...
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bioRxiv - Immunology 2020Quote: ... RAW 264.7 cells were seeded into 6-well plates at a density of 7.5 x 105 cells/well and incubated overnight with 200 U/mL of recombinant murine IFNγ (Biolegend, San Diego, CA) to promote M1-polarization and then infected with opsonized Cn for 2 h at 3:1 MOI or mock infected with PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2021Quote: ... 0.5 tests of anti-CD8 BV421 (Biolegend 344747), 1 test of anti-CD45 FITC (Biolegend 304005) ...
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bioRxiv - Immunology 2021Quote: ... injected with 0.5 mg/kg TNFα (Biolegend, CA). Blood was collected by cardiac puncture 6 hours or 2 hours after LPS or TNFα challenge ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with TruStain X plus (Biolegend) for 30 minutes followed by the extracellular antibodies (Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with TruStain X plus (Biolegend) for 30 min followed by the extracellular antibodies (CD80 ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with TruStain X plus (Biolegend) for 30 min followed by the extracellular antibodies (Table 1) ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Synthetic Biology 2022Quote: ... mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend) to a final concentration of 50 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... red blood cells (RBCs) were removed by lysis in 2 mL of RBC lysis buffer (BioLegend) for 3 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with 2 ug/ml PE/Cyanine7-conjugated anti-mouse TLR4 antibodies (BioLegend, 145407) or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed twice and blocked for 30 minutes with 50 uL FACS buffer (0.5% BSA in PBS) with TruStain FcX solution (0.5 uL/sample) (Cat # 422301, Biolegend, San Diego, CA). Antibodies were then added to 100 uL of BD Brilliant Stain Buffer (Cat # 659611 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 594 or Alexa Fluor 647 conjugated anti-mouse CD31 (2 μg/mL; clone MEC13.3; BioLegend) was used ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Immunology 2021Quote: ... 0.5 tests of anti-CD4 PE/Cy7 (Biolegend 344611), 0.5 tests of anti-CD8 BV421 (Biolegend 344747) ...
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bioRxiv - Immunology 2021Quote: ... and 0.5 tests of anti-CD14 AlexaFluor700 (Biolegend 367113) per million cells ...
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bioRxiv - Neuroscience 2022Quote: ... Rat anti-P2Y12 receptor (0.5 μg.μL-1, Biolegend 848002) was diluted in a solution of 0.125% gelatin and 0.01% triton in PB and samples were incubated for 72 h at 4°C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
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bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
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bioRxiv - Immunology 2023Quote: ... Th17 differentiation media consisted of complete media supplemented with 2 μg/mL anti-IL-4 (Cat# 504101; Biolegend), 2 μg/mL anti-IFNγ (Cat# BE0055 ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
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bioRxiv - Neuroscience 2020Quote: ... and PE anti-mouse CD19 (clone 6D5, 0.5 μg, BioLegend). For splenocyte isolation and staining ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% BSA and Cell Staining Buffer (Biolegend®, California, USA). Cells were incubated with an Anti-Hu/Mo Phospho-S6 (Ser 235 ...
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bioRxiv - Immunology 2022Quote: ... 0.5 ug each of oligo tagged CD4 (TotalSeqTM-C0072, Biolegend), CD8 (TotalSeqTM-C0046 ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-P2Y12 receptor (0.5 μg.μL-1, Biolegend 848002) were diluted in PGT buffer (0.125% gelatin and 0.1% triton in PB) ...
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bioRxiv - Immunology 2023Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2020Quote: ... and different concentrations of Tregs (1:2-1:64 Treg: Teff ratio, 500-2000 Tregs) were activated with 2μg/ml anti-CD3 (Biolegend) in a 96-well round bottom plate with 100ul RPMI for 72hrs (Turnis et al. ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...