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Citations for BioLegend :
151 - 200 of 2412 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and IFN-γ (2 μg/ml APC a-mouse IFN-γ, Biolegend) prior to analysis on a flow cytometer (LSR Fortessa X-20 ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Immunology 2019Quote: ... 2 ml medium/well) were cultured in growth media supplemented with GM-CSF (100 ng/ml, BioLegend, San Diego), TGF-β (10 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Genomics 2024Quote: ... at a ratio of 1:1 (cell:Dynabead) in the presence of 5ng/mL rhIL-2 and 10ng/mL rhIL-7 (Biolegend) in 96 well U-bottom plates ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2, Biolegend). RNAi knockdown of DCAF1 was performed as described before52 using ON-TARGET plusHuman DCAF1 siRNA - SMARTpool (Dharmacon ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μg/mL anti-IL-2 Alexa Fluor 488 (Biolegend, Catalog: 500314) in 50μL washing buffer per 6 μL nanovial volume at 37°C for 30 minutes protected from light ...
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bioRxiv - Immunology 2020Quote: ... 2 μg/ml rat anti-mouse CD40 antibody (BioLegend, San Diego, California, USA), 2 ng/ml interleukin 7 (IL7 ...
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bioRxiv - Immunology 2021Quote: ... and seeded into 2 μg/mL anti-CD3 antibody (clone: 145-2C11, BioLegend) pre-coated 24-well plates ...
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bioRxiv - Microbiology 2023Quote: ... 10 µM each) and 50 U/mL of recombinant mouse IL-2 (BioLegend) overnight ...
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bioRxiv - Biophysics 2024Quote: ... 100 µL of PE donkey anti-rabbit IgG (BioLegend #406421; 2 ug/mL) for GST-LANA ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µL of PE goat anti-mouse IgG (BioLegend #405307; 2 ug/mL) added and incubated in the dark for 30 min with shaking (800 rpm) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 ng/μl DAPI (Biolegend, 422801), 0.4 μM FITC-labeled and 0.2 μM Cy3- and Cy5-labeled detection probes for 1 hour at R/T ...
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bioRxiv - Immunology 2023Quote: ... using 0.5 µl HTO antibody (BioLegend) per sample ...
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bioRxiv - Immunology 2020Quote: ... and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL; BioLegend), or a cocktail of IL-12 (10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... 0.1% 2-mercapto-ethanol 1000X solution) supplemented with IL-7 (10 ng/mL, Biolegend). To activate T cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed with 2 ml of cell staining buffer (Cat. #420201, BioLegend), resuspended in 300 μl PBS and measured using an LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... These microglia were labeled with FITC anti-mouse CD45 (2 µg/mL, Biolegend, USA), APC anti-mouse CD11b (5 µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μM (in vitro) and neutralizing HMGB1 of 10µg/ml were obtained from Biolegend (isotype control mouse IgG2a kappa ...
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bioRxiv - Microbiology 2019Quote: ... the splenocytes were incubated for 2 h with 5 μg/ml brefeldin A (Biolegend) for intracellular trapping of cytokines and then stained with Zombie Aqua (1:200 ...
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bioRxiv - Immunology 2022Quote: ... or SARS-CoV-2 nucleocapsid peptide pool together with 5μg/ml Brefeldin A (Biolegend) for 16 hours ...
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bioRxiv - Immunology 2022Quote: ... media was changed to complete EGM-2 with 1 ng/mL TNFα (Biolegend 575206) and shear stress of 1 dyn/cm2 was incorporated using a microfluidic pump (Ibidi 10902 ...
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bioRxiv - Immunology 2023Quote: ... Cells were isolated from blood by adding 2 mL of RBC lysis buffer (Biolegend) per 100 uL of blood collected ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Biophysics 2023Quote: Antibodies uptake was determined by measuring the fluorescent intensity of a fluorescently-tagged antibody (Alexa Fluor® 647 anti-Tubulin-α, 0.5 mg/mL, BioLegend 627908) at a dilution ratio of 1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...