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Citations for BioLegend :
201 - 250 of 9088 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Th1 differentiation was achieved using 1 μg/mL anti-IL-4 antibody (clone: 11B11, Biolegend), 5 ng/mL IL-2 (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4°C overnight pERK antibody (1:20 dilution, Biolegend Cat. #675504). Samples were washed and resuspended for analysis ...
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bioRxiv - Immunology 2021Quote: ... Undifferentiated donor 2395 iPSC were stained with anti-SSEA-4-APC and anti-Oct-4-PE (BioLegend, San Diego, CA) using the Fixation/Permeabilization Solution kit from BD Biosciences (San Jose ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411 ...
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bioRxiv - Cancer Biology 2024Quote: ... for 10 minutes at 4°C and then surfaced-stained for 30 minutes at 4°C with the following antibodies obtained from BioLegend: anti-CD3-APC (Cat ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-mouse IL-4 (Cat: 504127 Biolegend), APC-Cy7 anti-mouse CD3 (Cat ...
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bioRxiv - Immunology 2021Quote: IL-4 or IL-13 (Biolegend or PeproTech) was added into macrophage cultures at various concentrations to induce the M2/M2a phenotype in the presence or absence of a soluble CD47 extracellular domain (mCD47.ex or hCD47.ex) ...
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bioRxiv - Cancer Biology 2021Quote: ... PE antihuman CD262 (DR5) (Biolegend, Clone DJR2-4), PE anti-human TRAILR3 (DcR1 ...
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bioRxiv - Immunology 2020Quote: ... and anti-4-1BB–BV421 (clone 4B41; BioLegend).
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bioRxiv - Cell Biology 2021Quote: ELISA assay of different cytokines IL-4 (BioLegend), IL-5 (BioLegend ...
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bioRxiv - Bioengineering 2022Quote: ... or 20 ng/mL IL-4 (BioLegend 574302) for 48 h prior to use in tumouroid assays or prior to analysis of protein expression by immunofluorescence microscopy or flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-4 BV421 (Biolegend, clone MP4-25D2), and anti-IL-21 eFluor660 (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... and 10 μg/ml anti–IL-4 (Biolegend) One week after initial activation resting NR1 cells were co-incubated with untreated or IFNγ-treated macrophages of different genotypes ...
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bioRxiv - Immunology 2020Quote: ... APC Rat anti-mouse CD11a (M17/4, BioLegend), PE Armenian Hamster anti-mouse CD103 (2E7 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of anti-HA antibody (BioLegend, 901513) was added to the remaining cleared lysate and incubated for 4 hours at 4 °C on an end-over-end rotator ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IL-4 (10 ug/mL, BioLegend), and anti- mouse IFN-γ (10 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... or anti- CTLA-4-APC (Mouse IgG2a, Biolegend) at 37°C for 1hour ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μl of the monoclonal antibody 8WG16 (Biolegend) were added for 1.5 h at RT followed by 1 h incubation with Protein G Dynabeads ...
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bioRxiv - Molecular Biology 2023Quote: ... or 4 μg of α-RPB1 (Biolegend 8WG16).
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bioRxiv - Cancer Biology 2024Quote: ... or 20 ng/ ml IL-4 (BioLegend, 574304) for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and DR5 (DJR2-4-PE, Cat:307406, Biolegend) markers for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and anti-CD28 (4 μg/ml, 37.51, BioLegend). Plates were washed with PBS and mouse naïve CD4+ T cells were cultured in the wells at 1x106 cells/ml in R10 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Intracellular antibodies: CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... FITC-conjugated anti-CD155 (clone SKII.4, BioLegend), FITC-conjugated anti- DNAM-1 (clone 11A8 ...
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bioRxiv - Immunology 2022Quote: ... and anti-IL-4-PE (clone 11B11, BioLegend), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... PE anti-human DR5 (clone DJR2-4; Biolegend), PE anti-human Fas (clone DX2 ...
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bioRxiv - Immunology 2022Quote: ... and anti-RT1D (BioLegend, #110211, 14-4-4S). Cells were subsequently fixed using IC fixation buffer (eBiosciences™ ...
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bioRxiv - Immunology 2024Quote: ... CD4 Alexa Fluor 700 (clone RM4-4, BioLegend), CD4 BUV737 (clone GK1.5 ...
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bioRxiv - Immunology 2024Quote: ... CTLA-4(BNI3)-PE-Dazzle (BioLegend (San Diego)) ...
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bioRxiv - Immunology 2024Quote: ... or with IL-4 (10ng/mL; BioLegend, 574304) and IL-13 (10ng/mL ...
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bioRxiv - Microbiology 2024Quote: ... and 25 ng/mL IL-4 (Biolegend, 574006). Monocytes were cultured on tissue culture treated T75 flasks (VWR ...
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bioRxiv - Microbiology 2024Quote: ... and 25 ng/mL IL-4 (Biolegend, 572902). Media was replaced with fresh cytokine-containing media every 3 days ...
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bioRxiv - Immunology 2024Quote: ... recombinant mouse IL-4 (60 ng/mL42; BioLegend) or both IL-12 and IL-4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of APC Annexin V (#640941, BioLegend) and 4 µl of 7-AAD (#420404 BioLegend) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4 µl of 7-AAD (#420404 BioLegend). Cells were incubated in the dark on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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bioRxiv - Immunology 2024Quote: ... The sections were then rinsed in a washing solution twice before nuclei were stained with 4’,6-diamidino-2-phenylidole (DAPI; BioLegend). The sections were rinsed with washing solution one final time and then mounted on slides for examination under an Olympus FV1000 laser-scanning 4-channel confocal microscope (Olympus).
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... The fluorochrome-conjugated antibodies were as follows for flow cytometry: BV605 Rat Anti-Mouse CD4 (RM4-5/RM4-4; Biolegend #116027), PE-Cy™7 Rat Anti-Mouse CD8a (53-6.7 ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...