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Citations for BioLegend :
351 - 400 of 9088 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with anti-mouse IL-4 antibody (11B11, Biolegend) then blocked as above ...
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bioRxiv - Immunology 2022Quote: ... and 4 μL of anti-CD193-PerCP/Cy5.5 (clone 5E8, BioLegend), and incubated the sample for ~18 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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bioRxiv - Immunology 2020Quote: ... Bcl6 PE/Dazzle (clone 7D1, BioLegend, cat# 358510, dilution 4:100). Data was recorded on a FACSymphonyTM flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) and CD124 (I015F8) mAbs were purchased from BioLegend. Anti-CD44 (IM-7) ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain) ...
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bioRxiv - Immunology 2020Quote: ... or 40 ng/mL IL-4 (Biolegend, San Diego, CA, USA) in duplicates respectively for 24 hours in X-VIVO 15 serum free medium ...
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bioRxiv - Pathology 2023Quote: ... Neutrophils were maintained 4 hours in culture with Brefeldin A (Biolegend) to stop exocytosis.
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% Triton-X and 4 µg/mL DAPI (#422801, BioLegend, USA) for 1 h at RT and stained with two staining panels ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde (Biolegend, San Diego, CA, USA) for 10 minutes and subsequently permeabilized with 0.1% Triton X100 (Merck KGaA ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend, catalog no. 504118), and Brilliant Violet 711 anti-mouse IL-17A Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-4 (#500808) was from BioLegend (San Diego, CA, USA). Cells detached from culture plates were filtered through 70-μm cell strainers ...
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bioRxiv - Immunology 2022Quote: ... and 10 μg/mL anti-IL-4 Ab (clone 11B11, BioLegend) were added to the culture media ...
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bioRxiv - Cancer Biology 2022Quote: ... we stained the cells with 4 μg/ml PE-streptavidin (BioLegend) at the 37 °C for 45 min ...
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bioRxiv - Immunology 2022Quote: Mouse splenocytes were stained with 4 μg/ml LAG3 BV785 (BioLegend) and 4 μg/ml CD49b PeCy7 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... TotalSeq™-A0304 anti-mouse Hashtag 4 Antibody) (BioLegend, TotalseqA antibodies). The 4 biological replicates of each time point were stained with one of the 4 unique hash antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... FITC anti-mouse CD4 Antibody (BioLegend, catalog #116003, clone RM4-4), PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... KO iv-: TotalSeq-A0304 anti-mouse Hashtag 4 (all from Biolegend). Equal number of cells from each population were pooled and then subjected to 10X single cell RNA seq library preparation targeting 1×104 total cells ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells from the bone marrow were first stained with the following combination of biotinylated antibodies (lineage cocktail) for 30 minutes at 4 C: Gr-1 (RB6-8C5; Biolegend), Ter-119 (TER-119 ...
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bioRxiv - Immunology 2023Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 4°C with KI67 primary antibody (cell signaling technology) diluted 1:400 or CD8a primary antibody (Biolegend) diluted 1:200 in Antibody diluent signal stain (Dako) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were washed in TBS-T and incubated overnight at 4 ◦C with monoclonal pNF-κB p65 (Ser 529)-specific antibody (1:500 dilution, clone 817403, Biolegend), monoclonal NF-κB p65-specific antibody (1:2500 dilution ...