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Citations for BioLegend :
2401 - 2450 of 9012 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL anti-CD28 (clone CD28.2: #302934, BioLegend) mAb ...
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bioRxiv - Immunology 2024Quote: ... The cytokines listed were purchased from BioLegend (Supplemental Table 5). Afterward ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human TruStain FcX (BioLegend, Cat#422301; 5 μl/sample) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-KRT5 polyclonal antibody [5 mg/ml] (905501, Biolegend), rabbit anti-SOX2 polyclonal antibody [5 mg/ml] (NB110-37235 ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 605 anti-mouse CD4 (clone RM4-5, BioLegend), APC anti-mouse IFN-γ (clone XMG1.2 ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 μg/ml anti-mouse CD180 antibody (Biolegend, 117708).
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bioRxiv - Immunology 2022Quote: ... and incubated with 5 μl of CD107a antibody (Biolegend, H4A3) and 5 μl of anti-CD3/CD28 beads ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5 μl of anti-CD326 (EpCAM)-PE-Cy7 (Biolegend) and incubated for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... anti-CD4-BV650 (clone RM4-5, BioLegend 100546, diluted 200×), anti-TCRβ-FITC (clone H57-597 ...
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bioRxiv - Microbiology 2023Quote: ... anti-CD64 (BV421, clone X54-5/7.1, cat. 139309, BioLegend), anti-MHCII (BV650 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ tetramethyl benzidine (TMB) substrate (BioLegend, San Diego, CA, USA) was added ...
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bioRxiv - Immunology 2023Quote: ... CD64-Brilliant Violet 421 (clone X54-5/7.1, Biolegend, 139309), Ly6G-PerCP-Cy5.5 (clone 1A8 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CRTAM (clone 11-5, Biolegend, San Diego, CA), hamster anti-mouse PD-1 clone J43 ...
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bioRxiv - Immunology 2023Quote: ... PECy7-anti-mouse CD4 (BioLegend, clone RM4-5, Cat#100528), APCCy7-anti-mouse CD8 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and plate-bound anti-CD3 antibody (5 μg/mL; Biolegend). For the induction of IL-22-expressing Th17 cells ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Immunology 2021Quote: Cryopreserved apheresis products were thawed and pre-labelled with antibodies for flow cytometry (described below) and a panel of TotalSeq™-B antibodies (described below) from Biolegend (San Diego, CA, USA). The panel of TotalSeq™-B antibodies included CD127 ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for B cells: anti-CD19 Alexa Fluor® 488 (BioLegend catalog #302219, clone HIB19), anti-CD3 PerCP/Cy5.5 (TONBO catalog #65-0037-T100 ...
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bioRxiv - Immunology 2019Quote: ... Plasma IL-6 levels were determined by sandwich enzyme-linked immunosorbent assay (ELISA) (Biolegend ELISA MAX Deluxe, London UK). Values below the blank were reported at the limit of detection for statistical purposes ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... male mice were isolated 6 dpi with NP-OVA/alum and stained with anti-CD4 Alexa Fluor 647 (GK1.5, BioLegend), anti-TCRβ PE-Cy7 (H57-597 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2022Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
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bioRxiv - Cancer Biology 2023Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
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bioRxiv - Immunology 2019Quote: ... Recombinant mouse CSF-2 (carrier free) was from BioLegend. L-α-phosphatidylcholine (PC ...
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bioRxiv - Genomics 2021Quote: ... anti-IL-2 (MQ1-17H12; PE Cy-7; Biolegend), and anti-MIP-1β (D21-1351 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-CD45.1/2 (clones A20 and 104, BioLegend) antibodies for 30 min (PB ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 (JES6-5H4) antibodies (all from Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... IL-2-BV750 (MQ1-17H12, BioLegend #566361, Lot 0042313), IL-4 BB700 (MP4-25D2 ...
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bioRxiv - Microbiology 2021Quote: ... Anti-human LAMP-2 (H4B4) was purchased from BioLegend. Anti-DIAPH2 (A300-079A-T ...
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bioRxiv - Cell Biology 2019Quote: ... Human IL-2 was quantified by ELISA (BioLegend, # 431804).
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bioRxiv - Microbiology 2021Quote: ... IL-2-PerCPCy5.5 diluted in perm-wash buffer (Biolegend). For analysis of NK cell intracellular cytokine production ...