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Citations for BioLegend :
2651 - 2700 of 9012 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Neuroscience 2019Quote: ... 1-16 (1:1000, Biolegend). Sections were viewed ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1 (1:200; BioLegend), and Ter119 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... PD-1 (BioLegend, 1:100), phopho-STING (ThermoFisher 1:100) ...
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bioRxiv - Immunology 2021Quote: ... 2 cell populations were sorted and barcoded separately with TotalSeq antibodies (BioLegend) before mixing and cell captures (Supplemental Data 1) ...
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bioRxiv - Immunology 2020Quote: ... antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days.
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bioRxiv - Biochemistry 2020Quote: ... and rat anti-mouse CD4 F(ab’)2 fragment (clone GK1.5, BioLegend, fragmented according to published procedures45 ...
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bioRxiv - Cell Biology 2021Quote: ... was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend) was used at 8 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend). The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... and CD8 (2 μg/ml APC/Cy7 a-mouse CD8a antibody; Biolegend) in PBS for 20 min before fixation in 2% paraformaldehyde (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... and IFN-γ (2 μg/ml APC a-mouse IFN-γ, Biolegend) prior to analysis on a flow cytometer (LSR Fortessa X-20 ...
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bioRxiv - Immunology 2021Quote: ... and anti-mouse-IL-2-PE-Cy7 (Biolegend Cat# 503832, RRID: AB_2561750). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences ...
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bioRxiv - Pathology 2021Quote: ... pan aSyn (N-terminal directed, SYN303 [aa 2-12 Cat# 82401, Biolegend] ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 μg/ml soluble anti-mouse CD28 antibody (102116, BioLegend, USA) in RPMI 1640 medium (21870-076 ...
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bioRxiv - Immunology 2021Quote: ... PE-anti-human-Nectin-2 (TX31) (all from BioLegend, San Diego, CA). The following antibodies were used for in vivo blocking experiments ...
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and cells were cultured with 0.5 µg/mL recombinant IL-2 (BioLegend) in RPMI + 10% heat-inactivated FBS for 24 h before use in NK cell cytotoxicity or flow cytometry experiments.
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bioRxiv - Biochemistry 2024Quote: ... An anti-pERK1/2 (Thr202/Tyr204) antibody (6B8B69) was purchased from BioLegend. Dead cells were stained with the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit for 633- or 635-nm excitation (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 2 hours after start of restimulation 5μg/mL of Brefeldin A (BioLegend) was added to each well for the remaining 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 96-well plates were coated with 5 g/ml of anti-CD28 antibody (Biolegend) o/n at 4°C to provide a co-stimulatory signal during co-culture ...
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bioRxiv - Neuroscience 2021Quote: ... The cell suspension was preincubated with TruStain FcX Plus Antibody (BioLegend, Supplementary Table 5) on ice for 10 minutes ...
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bioRxiv - Genomics 2020Quote: ... 564219) for 5 minutes and stained with directly conjugated anti-human CD45 (Biolegend 304006) and EPCAM (eBioscience 25-9326-42 ...
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bioRxiv - Immunology 2020Quote: ... PE-Anti-Mouse-CD254-(TRANCE-RANKL)-(IK22/5)-(510005) were acquired from Biolegend (USA).
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Pathology 2021Quote: ... mice were injected with 5 μg recombinant mouse IL-10 (Biolegend, San Diego, CA) or sterile saline 24 hours after APAP challenge ...
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bioRxiv - Cancer Biology 2020Quote: ... cytokeratin 5 (CK5) and 14 (CK14) (904801 and 905501 respectively, BioLegend, San Diego, CA). The tissue sections were counterstained ...
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bioRxiv - Immunology 2020Quote: ... IL-5 and IL-13) using the LEGENDplex mouse Th cytokine 13-plex (Biolegend) kit.
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were resuspended in 5 ml of red blood cell lysis solution (BioLegend) on ice for 5 min followed by the addition of 20 mL of complete media ...
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bioRxiv - Developmental Biology 2022Quote: ... Naïve hPSCs were passaged by 5 min incubation at 37 °C with Accutase (BioLegend). Primed hPSCs were cultured on plates pre-treated with 5 μg/ml Vitronectin (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed in PBS + 5% FBS and fixed in FluoroFix buffer (422101, BioLegend) prior to analysis on the BD LSR II analyser.
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bioRxiv - Cancer Biology 2022Quote: ... Brilliant Violet 421-conjugated rat anti-CCR7 (5 μL/10×106 cells; Biolegend, 120120) and Cells were incubated for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... or AF488 Mouse IgG1 κ isotype control (5 μg/mL, Biolegend, Cat. No. 400129) in 1x Intracellular Staining Permeabilization Wash Buffer (Perm/Wash ...
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bioRxiv - Cell Biology 2024Quote: ... T cell activation was done on anti-CD3 (5 μg/ml) (145-2C11, BioLegend), anti-CD28 (5 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Staining was performed using 5 µg/ml anti-human CD106 APC Antibody (Biolegend, USA) or 5 µg/ml mouse IgG2a kappa Isotype control (eBM2a ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend, 344716) per 6 μL nanovial volume ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor tissue was chopped finely with a razor in 5 ml Accutase (Biolegend, #4232201) and incubated at 37°C water bath for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Physiology 2023Quote: ... cells were resuspended in 5% FBS/PBS with Fc-blocking antibody (TruStain FcX; BioLegend) at 0.01 mg/ml for 5 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and 5 μL of APC anti-human CD144 (VE-Cadherin) antibody (clone BV9, BioLegend) to assess EC purity ...