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Citations for BioLegend :
2351 - 2400 of 4248 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control ...
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bioRxiv - Immunology 2024Quote: ... Cells were subsequently incubated with a cocktail containing Human TruStainFcX (Biolegend) and PBS-57/CD1d-tetramers (NIH Tetramer Facility ...
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bioRxiv - Bioengineering 2024Quote: ... and APC anti-human EGFR antibody (BioLegend catalog #352906, clone AY13) at 1:100 dilutions in DPBS + 1% BSA for 15 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse/human CD11b-PE/Dazzle 594 (1:120, 101256, Biolegend), and anti-mouse MHCII APC/Cyanine7 (1:120 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by staining with anti-human CD3 (UCHT1, BV650, BioLegend, #00467) and DAPI (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... FITC or PE-conjugated Mouse anti-human CD4 (clone OKT4; Biolegend) were used for cell-surface staining of HIV-1-infected primary CD4+ T cells ...
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bioRxiv - Immunology 2024Quote: ... and the PE anti-mouse/human CD11b antibody (Biolegend; 1:500) for 30 minutes at 40C ...
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bioRxiv - Immunology 2024Quote: ... and the PE anti-mouse/human CD11b antibody (Biolegend; 1:500) for 30 minutes at 40C ...
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bioRxiv - Immunology 2024Quote: ... PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4, BioLegend, cat. 317428); BrillianViolet conjugated anti-human CD25 (clone BC56 ...
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bioRxiv - Immunology 2024Quote: ... and PE conjugated anti-mouse/human CD11b (1:200, Biolegend, 101207) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... AF700 rat anti-human/mouse CD44 (clone IM7, BioLegend, cat# 103026) (54) ...
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bioRxiv - Immunology 2024Quote: ... or Alexa Fluor 647 anti-human CD206 (MMR) Antibody (BioLegend, #321116) in dark ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were magnetically labeled using MojoSort Human CD14 Nanobeads (Biolegend) and subsequently separated on LS columns using a QuadroMACS™ Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... cells were analyzed using Biolegend’s LEGENDScreenTM Human PE Kit (Biolegend, 700007). Seven subjects were incubated with IL-15 and IL-21 (as above ...
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bioRxiv - Immunology 2019Quote: ... Plasma IL-6 levels were determined by sandwich enzyme-linked immunosorbent assay (ELISA) (Biolegend ELISA MAX Deluxe, London UK). Values below the blank were reported at the limit of detection for statistical purposes ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... TNF-α and IL-2 levels were quantified in co-culture supernatants by ELISA (BioLegend, San Diego, CA, USA).
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.
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bioRxiv - Immunology 2019Quote: TNF-α and IL-6 were measured in the media of activated BMDMs using ELISA kits purchased from Biolegend per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... cell free supernatant was collected after 24 hours and IL-8 was quantified by ELISA according to manufacturer’s instructions (BioLegend).
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... in the presence of plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (0.5 μg/ml) supplemented or not with IL-27 (30 ng/ml, BioLegend). Proliferation of Tconv cells was determined by CellTrace Violet Dye dilution in FACS analysis ...
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bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1 μg/mL capture antibodies (IFNγ: clone R4-6A2, BD Pharmingen; or IL-17A: clone TC11-18H10.1, Biolegend) diluted in carbonate buffer ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Immunology 2023Quote: ... injected at 0.5 mg per mouse: rat anti-mouse CD210 (IL-10R, clone 1B1.3a, BioLegend, Cat# 112712, Lot# B360179,) and rat IgG1 ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605™ anti-human CD45 (HI30) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-human CD326 PE (BioLegend; Cat#324206; used at 1:200), and mouse IgM anti-human HTII-280 (Terrace Biotech ...
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bioRxiv - Immunology 2021Quote: ... 100 μl anti-human IgG or IgM - horseradish peroxidase (HRP) conjugates (BioLegend), diluted 1:2500 and 1:5000 in dilution buffer ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD309 (KDR/VEG-FR2) (Biolegend, clone 7D4-6; 1:200); anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human FcER1 BV510 (1:100, clone AER-37, BioLegend, cat # 334626), Anti-human CD303 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD163 PE (1:50, clone GHI/61, BioLegend, cat # 333606), Anti-human CD49d PE Dazzle 594 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD56 APC Cy7 (1:50, clone HCD56, BioLegend, cat # 318332), Anti-human CD45 APC Cy7 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human PD-1 BV785 (1:50, clone EH12.2H7, BioLegend, cat # 329930), Anti-human CD45 BUV395 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD4 BV510 (1:100, clone RPA-T4, BioLegend, cat # 300546), Anti-human CD56 BV605 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human MerTK PE Cy7 (1:50, clone 590H11G1E3, BioLegend, cat # 367610), Anti-human TIGIT PE Cy 7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD103 BV711 (1:100, clone Ber-ACT8, BioLegend, cat # 350222), Anti-human CD10 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...