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Citations for BioLegend :
2601 - 2650 of 4248 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and labeled with CFSE (5 M, Biolegend). 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... NKp46 (Biolegend, Clone 9E2: PerCP-Cy5-5), NKp30 (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... APC-labeled CD64 (X54-5/7.1, BioLegend), BUV563-labeled Ly6G (1A8 ...
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bioRxiv - Immunology 2024Quote: ... TotalSeq-A-labeled CD4 (RM4-5, BioLegend), TotalSeq-A-labeled ST2 (DIH9 ...
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bioRxiv - Immunology 2024Quote: ... CD64-PE/Cy7 (X54-5/7.1, BioLegend), Ly6C-BV650 (HK1.4 ...
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bioRxiv - Immunology 2024Quote: ... APC-labeled CD64 (X54-5/7.1, BioLegend), and TotalSeq-A-labeled Ly6G (1A8 ...
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bioRxiv - Immunology 2024Quote: ... TotalSeq-A-labeled CD4 (RM4-5, BioLegend), TotalSeq-A-labeled ST2 (DIH9 ...
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bioRxiv - Biochemistry 2024Quote: ... mCD4-FITC (clone RM4-5; BioLegend 100509), mCD8a-PE (clone KT15 ...
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bioRxiv - Immunology 2021Quote: ... 211-11A; Pepro Tech), IL-1β (1 ng/ml, 211-11B; Pepro Tech) or IFNα (100 ng/ml, 752802; Biolegend). Cytokines used in Figure 1 - figure supplement 1D were added at the concertation shown in Supplementary Table 1.
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Immunology 2020Quote: ... and 1.0-2.5 x 104 NK cells were cultured in K10 media along with either IL-15 alone (1-100 ng/mL; BioLegend), or a cocktail of IL-12 (10 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2019Quote: ... Some cultures were supplemented with combinations of 8µg/ml IL-34 (Generon, UK), 100 µg/ml BMP7 (RD systems, USA and 10µg/ml CSF-1 (Biolegend, USA) for the duration of the culture.
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bioRxiv - Physiology 2019Quote: ... Cell culture supernatant was collected and frozen for IL-2 analysis using LEGENDplex™ technology according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Immunology 2021Quote: ... Primary bone marrow-derived murine macrophages (BMDMs) were pretreated with PBS or murine IL-27 (50 ng/ml from BioLegend) for 24 hours and then infected with S ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... Phospho-protein stain was performed using an optimized protocol as follows: Following stain with antibodies against surface markers CD4 and IL-23R (12B2B64, Biolegend), the cells were fixed by 2% paraformaldehyde for 10 min at room temperature and washed once with cold PBS ...
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bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: DCs were differentiated from bone marrow precursors obtained from naive BALB/c mice in the presence of murine GM-CSF (10 ng/ml) and IL-4 (10 ng/ml) both from Biolegend for 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin for 16 h before exposure to recombinant mouse IL-6 (rIL-6) at 50 ng/ml (575702, BioLegend) or vehicle (PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Immunology 2021Quote: ... Peritoneal lavage cells were isolated and stimulated overnight at 37 °C with 50 ng/ml m-IL-1β (Biolegend, Cat# 575002) plus m-IL-23 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2019Quote: ... cells were permeabilized and stained with IL-10 (clone JES5-16E3, eBioscience) or isotype control (Rat IgG2b Κ Isotype Control, Biolegend). Fluorescence intensities were measured on a FACSCalibur and at least 25,000 events for lung tissue were analyzed using FlowJo.
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Immunology 2024Quote: ... PE-Cyanine7-conjugated anti-mouse Ly6G (E-AB-F1108H, elabscience, China) or intracellular staining for 488-conjugated anti-mouse IL-17A (506910, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-human CD31 APC-Cy7 (BioLegend; Cat#303120; used at 1:200), mouse anti-human CD326 PE (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend, 101228, clone M1/70, 1:100), APC anti-mouse F4/80 (BioLegend ...