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Citations for BioLegend :
2301 - 2350 of 2811 citations for Recombinant Human TGFB2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... aureus Newman (from 2.106 to 2.104/well) in the presence of anti-human CD107a-AF647 mAb (clone H4B4, Biolegend, France) added at the beginning of stimulation in serum and antibiotic free RPMI media in 96 well round bottom plates ...
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bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated overnight at 4 °C on a tube rotator at 18 rpm in 800 μL of PBS/0.2% BSA containing 15 nM human IgG1 (BioLegend) that had been conjugated with Alexa647 using NHS chemistry (Molecular Probes ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... single cell suspensions were incubated for 15 minutes at room temperature in the dark with Human TruStain FcX (Biolegend) at a concentration of 5µL per million cells to block unspecific Fc Receptor binding ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were stained with live dye (Calcein AM) and PE anti-human CD45 antibody (BioLegend, cat no 368510) mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100,000 IFNγ-treated tumor cells were seeded together with 200,000 pTc or TiTc per well in Tc medium containing anti-human CD107a-FITC (Biolegend). After 1h incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
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bioRxiv - Systems Biology 2021Quote: ... surface proteins were stained with a cocktail of 53 TotalSeq-C antibodies (Biolegend). Antibody concentrations were either predetermined by titration (Kotliarov et al. ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Microbiology 2020Quote: ... Protein G agorose beads that were pre-adsorbed to either anti-HA (BioLegend) or anti-FLAG (Millipore ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Immunology 2022Quote: ... Protein concentrations of inflammatory cytokines were determined by LegendPlex™ Multiplex Assay (BioLegend) using the pre-defined mouse inflammation panel according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2019Quote: ... Type III IFN proteins were measured using a custom-designed LEGENDplex kit (BioLegend). IFN-β was measured using the Verikine ELISA (PBL) ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with antibodies against surface proteins CD3 (APC-fire, BioLegend), CD11b (APC Cy7 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to detect immunoprecipitated proteins were as follows: Eos (1:500, Biolegend, W15169A), STAT5 (1:5000 ...
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bioRxiv - Immunology 2023Quote: ... whereas ADT libraries (cell surface protein libraries) were processed following instructions from Biolegend. 3’ gene expression and ADT libraries were quantified by fragment analyzer and pooled for multiplexed sequencing on a NovaSeq 6000 instrument by the Deep-sequencing Facility at the Max Planck Institute for Immunobiology and Epigenetics ...
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bioRxiv - Immunology 2024Quote: ... biotinylated gE proteins (Acro Biosystems) were conjugated with BV421- or APC-streptavidin (Biolegend) at a molar ratio of 4:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus, referred to as anti-h/pCD47; Biolegend). The level of cell surface CD47 is expressed as median fluorescent intensity (MFI) ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for coating of culture plates for in vitro T cell activation: anti-human CD3 (clone HIT3α; Biolegend), anti-human CD28 (clone CD28.2 ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... MCF-7 cells in EPB were stained with a 1:20 dilution of FITC Anti-human CD326 (Biolegend, Cat.no. 324204) for 30 min at 4 °C to allow for population tracking in flow cytometry ...
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... or 1 million cells (analysis)No in FACS buffer (PBS with 2% FBS, 1X pen/strep and 1 mM EDTA) containing human TruStain FcX (Biolegend) and mouse TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2021Quote: ... Viability marker was washed out and cells were resuspended and spun down in FACS buffer then incubated with Human TruStain FcX (Biolegend) for 10 minutes prior to the addition or a surface antibody cocktail (Table S9 ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Genomics 2019Quote: ... Measurement of BCL-6 expression in targeted Jurkat lines was assessed by flow cytometry using anti-human APC-BCL-6 (Biolegend) after treatment with human recombinant IFNg (5 ng/mL ...
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bioRxiv - Genomics 2019Quote: ... Expression of Ikaros and CXCR5 by targeted Jurkat lines was also assessed by flow cytometry using anti-human APC-CXCR5 (Biolegend) and anti-human PE-Ikaros (BD Biosciences) ...