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Citations for BioLegend :
2201 - 2250 of 2811 citations for Recombinant Human TGFB2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ cells were further sorted by FACS for CD7+ using anti-human CD7-FITC antibody (CD6-6B7; BioLegend).
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of human cellsurface molecules: ①Purchased from Biolegend(USA): Anti-hCD36-PE(clone 5271).
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Immunology 2023Quote: ... 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Bioengineering 2023Quote: ... the primary monocytes were isolated using a monocyte isolation kit (MojosortTM Human CD14+ Monocytes Isolation Kit, BioLegend®), per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7- anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with primary antibodies to human phospho-6SRP (Ser235/236) (CellSignaling 4858) or CD45 (BioLegend 304002) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
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bioRxiv - Immunology 2024Quote: NK cells were defined as positive for CD56dim using Brilliant Violet 650 conjugated mouse anti-human CD56 (BioLegend) and PerCP/Cyanine5.5 conjugated mouse anti-human CD16 antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 400 mg/mL Pacific Blue-mouse-anti-human CD45 (BioLegend; Cat. No. 304029; clone HI30), 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and 1 mL 70 mg/mL BV421-mouse-anti-human CD103 (Biolegend; Cat. No. 350214; clone Ber-ACT8). After the incubation ...
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bioRxiv - Immunology 2024Quote: ... 100 µL 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) was added to each well ...
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bioRxiv - Genomics 2024Quote: ... incubated with 0.5ug of a unique Cell Hashing antibody (TotalseqTM-A0251 to A0257 anti-human hashtag antibody, BioLegend) for 20 minutes at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Bioengineering 2024Quote: ... TCR was detected using an anti-human TCR α/β antibody conjugate to BV421 (Biolegend, San Diego, CA). Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110 ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the extracellular staining according to the manufacturer’s protocol: PerCP/Cyanine5.5 anti-human CD3 Antibody (#300430, BioLegend), Brilliant Violet 421 anti-human CD4 Antibody (#357424 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-dephosphorylated neurofilament protein (clone smi32; Biolegend, San Diego, CA, USA); rabbit anti-Caspr1 (clone EPR7828 ...
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bioRxiv - Immunology 2022Quote: ... −phycoerythrin (PE) and −peridinin chlorophyll protein complex (PerCP) were purchased from Biolegend. Phospho-specific mAbs against p65 (S536 ...
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bioRxiv - Microbiology 2020Quote: ... antibodies against the following proteins and epitopes were used: HA (16B12, BioLegend), β-actin (AC-74 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated protein was then mixed with streptavidin fluorophores (AF647, BioLegend; BV421, BioLegend), as described previously6 ...
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bioRxiv - Genomics 2022Quote: ... Proteins of interest were visualized using an anti-Flag-PE (BioLegend, 637310), an anti-HA-Alexa647 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Immunology 2023Quote: ... WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend, 405275) and TotalSeq™-C0974 Streptavidin (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: The scFc protein was labeled with SNAP-Surface 488 (BG-488, Biolegend). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilised with 0.2 % Triton X in PBS and probed using anti-human CD63 antibody (Clone: H5C6, Biolegend Cat: 353005) directly conjugated to FITC and counterstained for polymerised actin using 0.2 × Alexa Fluor 555 Phalloidin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellet was further resuspended in 150ul PBS+0.5%BSA and stained with PE anti-human CD56 antibody (1:100, Biolegend) for 20min in dark ...
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bioRxiv - Immunology 2021Quote: Supernatants were tested for multiple cytokine production using the bead assay LEGENDplex Antivirus Human panel (Biolegend, San Diego, USA) or with a commercial Luminex multi-analyte assay (Biotechne ...
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bioRxiv - Immunology 2021Quote: ... in PBS) for 30 minutes with 1:400 dilution of FITC Mouse anti-Human HLA-A2 (clone BB7, Biolegend), APC anti-human HLA-A,B,C (clone W6/32 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with Brilliant Violet 421-labelled anti-human P2Y12 receptor antibody (clone S16001E, Biolegend, Cat# 392106) or with IgG2a isotype control antibody (clone MOPC-173 ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: ... IHC was performed to rat or human tissue with the following panel of antibodies: pan-Aβ (Biolegend, Cat.# 800709,), pE3-Aβ (Cell Signaling Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 100 µl 1:2500 diluted (1% NAP Blocker in PBS) HRP-conjugated anti-human IgG antibody (BioLegend #410902) or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once in PBS+0.2% BSA and stained for 30 min with anti-human STAT3 pTyr705-Alexa Fluor 647 (BioLegend) in PBS+0.2% BSA at RT ...