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Citations for BioLegend :
2251 - 2300 of 8531 citations for HCC 1 CCL14 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of human cellsurface molecules: ①Purchased from Biolegend(USA): Anti-hCD36-PE(clone 5271).
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... untreated AML patients were determined by enzyme-linked immunosorbent assay (ELISA) using an IL21 Human ELISA kit (Biolegend), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... the primary monocytes were isolated using a monocyte isolation kit (MojosortTM Human CD14+ Monocytes Isolation Kit, BioLegend®), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads M270-Epoxy (Thermo) was covalently conjugated with combinations of mouse anti-human CD3 antibody (clone UCHT1, BioLegend), mouse anti-human CD28 antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with primary antibodies to human phospho-6SRP (Ser235/236) (CellSignaling 4858) or CD45 (BioLegend 304002) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7-anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... cells were pretreated with an FcR blocker (Miltenyi Biotechnology, #130-092-575 for mouse; BioLegend, #422302 for human) for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilised with 0.2 % Triton X in PBS and probed using anti-human CD63 antibody (Clone: H5C6, Biolegend Cat: 353005) directly conjugated to FITC and counterstained for polymerised actin using 0.2 × Alexa Fluor 555 Phalloidin (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: Supernatants were tested for multiple cytokine production using the bead assay LEGENDplex Antivirus Human panel (Biolegend, San Diego, USA) or with a commercial Luminex multi-analyte assay (Biotechne ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with Brilliant Violet 421-labelled anti-human P2Y12 receptor antibody (clone S16001E, Biolegend, Cat# 392106) or with IgG2a isotype control antibody (clone MOPC-173 ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Biochemistry 2019Quote: ... IHC was performed to rat or human tissue with the following panel of antibodies: pan-Aβ (Biolegend, Cat.# 800709,), pE3-Aβ (Cell Signaling Technologies ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once in PBS+0.2% BSA and stained for 30 min with anti-human STAT3 pTyr705-Alexa Fluor 647 (BioLegend) in PBS+0.2% BSA at RT ...
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bioRxiv - Bioengineering 2020Quote: ... The remaining cellular content was stained with anti-human CD45 (PacBlue or PECy7, clone HI30, BioLegend #304029 or #304016) and biotinylated cetuximab ...
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bioRxiv - Neuroscience 2020Quote: ... APC-conjugated rat monoclonal anti-mouse CD11b and PerCP-Cy5.5-conjugated mouse monoclonal anti-human CD200 (all Biolegend, UK) on ice in the dark for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with an antibody that recognizes the luminal epitope of the human LAMP1 protein (clone H4A3, BioLegend) conjugated with Alexa fluor 488 or 647 for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... low endotoxin (Super affinity) (Acro) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend) and RFP expression ...
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bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T helper cells were sorted as naive T cells (CD4+CD25−CD45RA+) or memory T cells (CD4+CD25−CD45RA−) using the following antibodies: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4 ...
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bioRxiv - Genomics 2019Quote: ... Cells were labelled for CD47 expression with biotin anti-human CD47 antibody (clone CC2C6, BioLegend, Cat.# 323104, RRID: AB_756134) at 0.0625 µg/100 µl ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with FACS buffer (0.3% BSA in PBS) and stained with Human TruStain FcX (BioLegend, Cat# 422301) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647-labelled monoclonal mouse anti-human CD62P (P-Selectin) antibody (Clone AK4, BioLegend, San Diego, CA, USA), Alexa Fluor 647-labelled goat anti-mouse IgG (GAMIG AF-647 ...
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bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: To study the human HSPC phenotype of the gene edited cells were stained with hCD34-Pecy7 (581 clone, Biolegend), hCD38-PE (HB7 clone ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Bioengineering 2022Quote: ... the streptavidin modified nanovial solution was then mixed with a solution containing 8 μg/mL anti-human CD45 (Biolegend) at equal volume ...
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bioRxiv - Immunology 2022Quote: ... Fc block was performed with a combination of Fc block -Human Tru Stain Fc block (Catalogue No. 422301, Biolegend), Nova Block Solution ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Immunology 2022Quote: All surface staining was done in the presence of Human Fc block and Monocyte Blocker (Biolegend, San Diego, CA). Following staining ...
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bioRxiv - Immunology 2022Quote: ... low endotoxin (Super affinity) (Aero) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend). For cytotoxicity assay analysis ...
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bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... Cells were spun down and supernatant was frozen for Legendplex analysis (LEGENDplex Human CD8/NK Panel 13-plex, BioLegend, according to the manufacturer’s information) ...