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Citations for BioLegend :
2201 - 2250 of 8531 citations for HCC 1 CCL14 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and the production of IFN-γ and Granzyme B was checked with PerCP anti-human IFN-γ (BioLegend) and FITC anti-human Granzyme B (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with an anti-human CD271(ΔNGFR) PE-conjugated antibody (BioLegend, Cat. No. 345106, RRID: AB_2152647) (dilution 1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC and used for infection.
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bioRxiv - Cancer Biology 2022Quote: The cells used in this study were labeled with anti-human B7-H3 monoclonal antibody (Biolegend, clone MIH42) PerCP/Cyanine 5.5 conjugate at 4°C for 45 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... HIV-infected cells were previously incubated with 10 μg/ml of anti-human HLA-E (clone 3D12, Biolegend) antibody for 20 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the flow panel: mouse anti-human CD4 Alexa Fluor 700 (OKT4, Biolegend), mouse anti-human CD20 BV785 (2H7 ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Cancer Biology 2021Quote: ... The stained samples were then incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend). Post staining ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend) and data acquired on a BD Symphony A5 cytometer ...
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bioRxiv - Immunology 2019Quote: The following fluorescent conjugated antibodies were used: antibodies to human CD3ε-BUV395 or PeCy7 (UCHT1; eBioscience and Biolegend), CD4-PerCPCy5.5 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Immunology 2019Quote: ... Human MAIT cells were isolated by sorting CD2 MACS-enriched (Miltenyi) leukocytes with CD161 and Vα7.2 antibodies (BioLegend). MAIT cells were grown for around 6 weeks in complete RPMI media supplemented with IL-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... resuspended in FACS buffer (1X PBS containing 0.5% FBS and 0.01% sodium azide) and used for staining with the following anti-human antibodies (Biolegend): CD45 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Immunology 2021Quote: ... cells were also stained with Alexa Fluor 488 anti-human TCR α/β (306712, BioLegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Murine monoclonal FITC anti-human CD11b (activated) antibody (IgG1, clone CBRM1/5) was from Biolegend (San Diego, CA). Goat anti-mouse (GαM ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... permeabilized for 10 min in PBS/0.1% saponin and stained with FITC-conjugated anti-human Perforin (γG9, Biolegend) and PE-conjugated anti-human Perforin antibody (B-D 48 ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Cancer Biology 2022Quote: ... Blocking buffer was then removed and cells were incubated with an APC-conjugated anti-human EpCAM (BioLegend, 369809) antibody diluted 1:20 in dilution buffer (1x PBS-T ...
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bioRxiv - Bioengineering 2022Quote: ... Enriched CD34+ HSPCs were stained with APC anti-human CD34 antibodies (clone 581, Biolegend, San Jose, CA, USA), and sample purity was assessed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Genomics 2024Quote: ... incubated with 0.5ug of a unique Cell Hashing antibody (TotalseqTM-A0251 to A0257 anti-human hashtag antibody, BioLegend) for 20 minutes at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Bioengineering 2024Quote: ... TCR was detected using an anti-human TCR α/β antibody conjugate to BV421 (Biolegend, San Diego, CA). Flow cytometry to assess CAR and TCR positivity was performed on Day 8 of manufacturing on an Attune NxT flow cytometer (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110 ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the extracellular staining according to the manufacturer’s protocol: PerCP/Cyanine5.5 anti-human CD3 Antibody (#300430, BioLegend), Brilliant Violet 421 anti-human CD4 Antibody (#357424 ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Immunology 2024Quote: NK cells were defined as positive for CD56dim using Brilliant Violet 650 conjugated mouse anti-human CD56 (BioLegend) and PerCP/Cyanine5.5 conjugated mouse anti-human CD16 antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The IcAR-PD1 cell line was stained using PE-conjugated mouse anti-human PD1 antibody (cat: 379210, BioLegend). PE-conjugated mouse IgG2b (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with 2.5 μl of PE anti-human E-cadherin (50 μg/ml; Biolegend, 324106) or matched concentration of PE Mouse IgG1 ...
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cytokines were quantified using the LEGENDplex Human CD8/NK Panel 13-plex (Cat.No # 740267 Lot B330268) from Biolegend. This is a bead-based immunoassay able to quantify the concentration of different analytes in co-culture supernatants ...
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bioRxiv - Immunology 2023Quote: Surface expression of mouse or human CD47 on murine tumor cell lines was assessed using MIAP301-PE (Biolegend) and CC2C6-APC (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: Determination of human α-synuclein concentration in nodose ganglia was performed according to manufacter’s instructions (Biolegend, Cat#844101). The 1:10 diluted samples were run in triplicates and reference standard curve ranged from 6.1 – 1500 pg per mL ...
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bioRxiv - Microbiology 2023Quote: ... fluorescein isothiocyanate-conjugated polyclonal goat anti-human C3b antibody (ICN) and PE-conjugated goat anti-mouse IgG (Biolegend). For the neutrophil killing assays ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ cells were further sorted by FACS for CD7+ using anti-human CD7-FITC antibody (CD6-6B7; BioLegend).
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...