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Citations for BioLegend :
2101 - 2150 of 8128 citations for 3 Methyl 1 6 naphthyridine 2 carboximidamide hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 2 mM EDTA in PBS and stained with cell viability dye (Zombie Aqua or Zombie UV, BioLegend). Cells were then incubated with 10 μg/mL anti-CD16/CD32 (2.4G2 ...
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bioRxiv - Cancer Biology 2020Quote: Flow cytometry staining was performed using the antibodies in Supplementary Table 2 in addition to the Zombie Aqua viability stain (BioLegend) and the Foxp3 Transcription Factor Staining Buffer Set (eBioscience) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed 2 times then resuspended in 100 μL wash buffer with 0.1 μg/mL streptavidin-PE (Biolegend 405203) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... iMACs were then washed with PBS plus 2% FBS and incubated for 15 min at RT with 50 ul/mL Human TruStain FcX Blocking solution (BioLegend) in PBS + 2% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were first blocked with 2% mouse serum (eBioscience San Diego, California) and Human Fc Block (Biolegend San Diego, California) for 10 minutes on ice ...
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bioRxiv - Physiology 2023Quote: ... Non-cardiomyocytes resuspended in Cell Staining Buffer (phosphate-buffered saline containing 2% FBS and 0.1% sodium azide) were treated with anti-mouse CD16/CD32 antibody (BioLegend, #101302) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the spleen were prepared as described above and splenocytes were exposed to 2-μM Tag-it VioletTM Proliferation and Cell Tracking Dye (Biolegend) diluted in PBS supplemented with 0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The antibody solution was then removed and the isolated lymph node cells were added at 2×105 cells per well together with soluable anti-mouse CD28 (102112, Biolegend) at a final concentration of 2 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
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bioRxiv - Immunology 2022Quote: ... washed with FACS buffer (PBS supplemented with 1 mM EDTA and 2% fetal calf serum) and stained with a live/dead marker (viability-eF780, eBiosciences) together with CD19-AF700 (HIB19, BioLegend), CD20-PE-CF594 (2H7 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in FACS buffer (PBS plus 2% FBS) and blocked with monoclonal antibody to CD16/32 (Human TruStain FcX, #422302, Biolegend) for 10 min at 4°C before staining with surface antibodies ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Genomics 2023Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e. stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend®), or DMSO as control (i.e ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... BMDMs were stimulated for 2 hours prior to infection with vehicle or 10 ng/mL of each cytokine: GM- CSF (Biolegend), M-CSF (Miltenyi) ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions were resuspended in PBS with 2% Newborn Calf Serum and stained with TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Immunology 2024Quote: ... samples were stained on ice for 45 minutes in PBS with 2% Newborn Calf Serum and True-Stain Monocyte Blocker (BioLegend) using a combination of the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2024Quote: ... IL-2 (anti-human IL-2, PE-Cy7, clone MQ1-17H12), and CD154 (anti-human CD154, APC, clone 24-31) all from Biolegend in Perm wash buffer (PBS + 0.02% NaN3 + 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 100 µL of staining mix (10% FBS in FACS buffer) and 2 µL of allophycocyanin (APC) anti-HA.11 [clone 16B12] (BioLegend). This amount of antibody was empirically tested to yield the highest stain index ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of 10 ng/mL recombinant human IL-2 and 10 ng/mL of recombinant human IL-15 (BioLegend) for 3 days ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor 594 conjugated anti-hCD19 (mouse IgG1, R & D Systems) and Alexa Fluor 647 conjugated anti-human CD340 (erbB2/HER-2, Biolegend) antibodies were used for CEACAM5 ...
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bioRxiv - Immunology 2024Quote: ... 4×106 cells from spleen were plated in a 96-well plate and incubated with SIINFEKL peptide and anti-CD107a for 2 hours at 37°C before Brefeldin A (5.0 mg/mL: Biolegend, 420601) and Monensin protein transport inhibitors (4 pL/6E6 cells ...
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bioRxiv - Immunology 2024Quote: ... Specific IgG and IgM antibody levels were measured in culture supernatants by ELISA using LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgG ELISA kit (BioLegend) and LEGEND MAXTM SARS-CoV-2 Spike S1 Human IgM ELISA kit (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... Autologous CD8+ T cells were isolated from PBMCs using EasySep human CD8+ T cell isolation kit (TEMCELL, Cat# 17953) and stained with 2 μM CFSE (BioLegend) in PBS at r.t ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Immunology 2024Quote: PBMCs (2×107) of HFRS patients were stained with PE-Cy7-labeled anti-human CD3 mAb (Clone 17A2, BioLegend, USA), APC-Cy7-labeled anti-human CD56 mAb (Clone 5.1H11 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-IgD Pacific Blue (Clone IA6-2, 348224), anti-IgM BV570 (Clone MMH-88, 314517) and Zombie NIR fixable viability dye (423106, Biolegend). Cells were then washed in PBS (D8537 ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was used for ELISA analysis of IL-2 and latent TGF-beta secretion levels by the kits from Biolegend according to manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... the four-class iPSC dataset (Cell, Mitotic, Dying cell, Debris) was stained with PE anti-human TRA-1-81 (1:300, Biolegend, #330708, clone TRA-1-81), while the five-class iPSC morphology dataset was stained with Alexa Fluor ® 555 anti-GSK3 beta (1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were anti-tau monoclonal antibodies including DA9 (aa 102–140, 1:10 dilution), ET3 (aa 273-288, 1:10 dilution)57 and 77G7 (BioLegend, aa 316-355, 1:100 dilution). DA9 and ET3 antibodies were generously provided by Peter Davies ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
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bioRxiv - Cancer Biology 2022Quote: ... PE-Cy7-conjugated anti-PDPN (8.1.1, #127411, 1:100), APC-Cy7-conjugated anti-MHCII (M5/114.15.2, #107628, 1:300) (from BioLegend); FITC-conjugated Hypoxyprobe-1-MAb1 (4.3.11.3 ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific antibody was detected using biotinylated anti-mouse antibodies (anti-IgG1 [RMG1-1] 0.02μg/ml, anti-IgG2b [RMG2b-1] 0.05μg/ml, Biolegend, CA ...
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bioRxiv - Immunology 2020Quote: ... CD64(FcγRI)#BV711 (1/100; 139311) and IFNγ#PE/Cy7 (1/300; 505825) were from Biolegend.
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bioRxiv - Biochemistry 2020Quote: ... anti-tubulin antibodies E7 (DSHB, AB_528499, 1:1000) and/or TU-01 (BioLegend 625902, 1:1000), and anti-RNA Polymerase II (Abcam ...
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bioRxiv - Immunology 2020Quote: ... followed by a 1 h incubation with a 1:3000 dilution of HRP-labeled streptavidin (Biolegend) in Casein ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 1 μg/mL anti-CD28 and 1 ng/mL recombinant IL7 (BioLegend) for 48 hours ...
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bioRxiv - Immunology 2022Quote: ... the cell suspension was incubated in 5 μgml−1 anti-CD16/32 (#101302, Biolegend, 1:200), stained with Live Dead Near IR (#L10119,ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Ki67 (1:100, 14-5698-82, eBioscience™) and anti-CD31 (1:200, 102516, Biolegend). As secondary antibody Alexa Fluor 555 (1:1000 ...