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Citations for BioLegend :
2051 - 2100 of 8128 citations for 3 Methyl 1 6 naphthyridine 2 carboximidamide hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000).
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bioRxiv - Developmental Biology 2021Quote: ... 1:200 v/v Alexa Fluor 647 anti-mouse/human CD15 (SSEA-1) (Biolegend, 125608) and 1:500 v/v PE anti-mouse/rat CD61 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... β3-tubulin (1:1000, cat. no. 801202, Biolegend; 1:500, cat. no. 302 306, SySy) anti-GFP (IF 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-LAMP1 (DSHB, 1D4B; 1:250) and mouse anti-Neurofilament (BioLegend, 837801; 1:500); and the following secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... CD8-FITC (1:400) or CD8-APC/Cy7 (clone 53-6.7, Biolegend 100714, 1:400) or CD8-BV650 (clone 53-6.7 ...
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bioRxiv - Immunology 2020Quote: ... and PDL-1 PE-Cy7 to visualize the tolerogenic ligand PDL-1 (Biolegend, Catalog #: 124313). After staining for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... anti-F4/80 (clone BM8, 1:100, BioLegend; clone T45-2342, 1:100, BD Biosciences); anti-CD11b (clone M1/70 ...
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bioRxiv - Cell Biology 2022Quote: ... - αE (clone 2E7, dilution 1:200) (eBioscience), - αLβ2 (clone H155-78, dilution 1:200) (Biolegend), - αM (clone M1/70 ...
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bioRxiv - Immunology 2024Quote: ... cells were first stimulated with cell activation cocktail (1:500 or 1:2500) (Biolegend, 423301), BFA (5 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice and stained with pSTAT3[Tyr705]-PE (1:10; 13A3-1, BioLegend) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were 1:500 Iba1 (wako 019-19741) and 1:1000 6E10 (BioLegend 803004) diluted in 5% NDS in TBS ...
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bioRxiv - Immunology 2023Quote: ... and HLA-A3 PE-Vio770 (Miltenyi, 1:100) or HLA-C AF647 (BioLegend, 1:100). Stained cells were washed twice and acquired on a BD LSRII.
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-dynactin 1 (1:250, BioLegend, Cat#867602), mouse anti-dynein (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD7 (6B7; 1:20) and CD38 (HIT2; 1:20; both from BioLegend, San Diego, CA); CD10 (SJ5-1B4 ...
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bioRxiv - Developmental Biology 2023Quote: ... dilution 1:1000] and Alexa Fluor® 647 CD49f antibody (Biolegend #313610, dilution 1:20) and subsequently segregated into hormone-sensing and alveolar through the staining with Brilliant Violet 605™ Sca-1 antibody (Biolegend #108133 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD7 (6B7; 1:20) and CD38 (HIT2; 1:20; both from BioLegend, San Diego, CA); CD10 (SJ5-1B4 ...
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bioRxiv - Neuroscience 2023Quote: ... containing ssDNA (1 mg/mL final concentration) and FC block (1:100 of BioLegend, 156604), was added ...
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bioRxiv - Immunology 2024Quote: ... 1×106 freshly isolated cells were triple stained with CD11b-Apc (#101211, Biolegend; 1:100), Zombie NIRTM Fixable Viability Kit (#423105 ...
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bioRxiv - Immunology 2024Quote: Cultured 1 x 106 THP-1 is immediately fixed by adding Fixation buffer (BioLegend, 420801) pre-warmed to 37 °C ...
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bioRxiv - Immunology 2024Quote: ... CD8-FITC (eBioscience, 11-0083-85, 1:100) or CD4-FITC (Biolegend, 100406, 1:100). CD4+ T cells were recognized as CD45+Ly6-C/G-CD115-TCRβ+CD4+ or CD45+Ly6-C/G-CD115-TCRβ+CD8- ...
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bioRxiv - Synthetic Biology 2024Quote: ... Alexa Fluor 488 conjugated to anti-β-amyloid 1-16 6E10 (1:200) (Biolegend, 803001) with 488 Conjugation kit (1:200 ...
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bioRxiv - Immunology 2021Quote: ... containing the following fluorescently-labelled antibodies diluted in FACS buffer (2% FBS in PBS): CD4-APC-Cy7 (clone A161A1; BioLegend), CD8a-Alexa700 (clone SK1 ...
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bioRxiv - Immunology 2021Quote: 1-2 × 106 fresh decidual leukocytes were washed with PBS and stained using the following cocktail of antibodies: CD45 (HI30, Biolegend), CD66b (G10F5 ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... centrifuged at 200g for 2 minutes followed by ice-cold PBS and incubated with Zombie NIR Fixable Viability dye (Biolegend) to exclude dead cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed twice with PBS plus 2 % FBS and subsequently coated with 10 μg/ml unconjugated anti-CD16 (3GB, BioLegend) in PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2020Quote: ... Supernatant from eleven donors responding in the ELISPOT assay were profiled using a 12-plex Legendplex T Helper Cytokine Panel Version 2 (Biolegend) following the manufactures instructions ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Immunology 2021Quote: ... The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend) and WIV1-CoV RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2020Quote: ... fixed with 2% formaldehyde and permealized with BD Perm/Wash buffer (Fischer) and stained with anti IFN-γ (clone XMG1.2; BioLegend) and anti-GM-CSF (clone MP1-22E9 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 2 μg/mL of Alexa Fluor 647 labelled anti-CD5 antibody or anti-CD180 antibody (BioLegend) and 2 μg/mL of Alexa Fluor 488 labelled anti-IgM Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2021Quote: ... IgD-BV510 (clone 11-26c.2a), CD138-BV605 (clone 281-2), CD38-PE-Cy7 (clone 90, CD27-APC (clone LG-3A10) (Biolegend), CD4-FITC (clone GK1.5 ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...