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Citations for BioLegend :
2051 - 2100 of 3492 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Microbiology 2022Quote: ... total lymphocytes were counted and naïve B cells were isolated using Mojosort Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Immunology 2019Quote: ... anti-CD38– APC-Cy7 (clone HB-7; BioLegend), and anti-CD20–FITC (clone 2H7 ...
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bioRxiv - Cell Biology 2021Quote: ... surface staining was performed using 7-AAD (Biolegend) and fluorochrome-tagged antibodies to CD45 and CD117 (both from Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD45 PE Cyanine 7 (BioLegend Cat# 304125, RRID:AB_10709440), CD42b APC Cyanine7 (BioLegend Cat# 303920 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD49a PE/Cy7 (clone TS2/7, Biolegend), anti-CD45 allophycocyanin (APC ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-AAD (Biolegend) or ZOMBIE NIR (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... anti-GL-7-PE-Cy7 (cat.no:144620,Biolegend),anti-IgD-BV786 (cat.no ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were stained with 7-AAD (Biolegend) for live/dead discrimination ...
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bioRxiv - Developmental Biology 2019Quote: ... or CD44-PerCP (BioLegend, IM-7, 1:150), at 4°C for 30□min ...
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bioRxiv - Immunology 2019Quote: ... and CD56-Phycoerythrin Cyanine 7 (clone NCAM; BioLegend).
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bioRxiv - Microbiology 2019Quote: ... Cellular DNA was counterstained with 7-AAD (BioLegend) and the cells were analyzed on a BD LSR Fortessa instrument.
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bioRxiv - Immunology 2020Quote: ... 7-AAD viability staining solution was from Biolegend. MART-1 peptide (MART-126-35A27L ...
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bioRxiv - Systems Biology 2019Quote: ... trypsinized and stained with 7-AAD (BioLegend 420404) or Zoombie NIR (BioLegend 423105 ...
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bioRxiv - Immunology 2019Quote: ... anti-αV integrin RMV-7 was from BioLegend, and anti-Mac1 mAb M1/70 was purified from hybridoma supernatant ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using 7-AAD (Biolegend).
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bioRxiv - Immunology 2022Quote: ... anti-CD49a PE/Cy7 (clone TS2/7, Biolegend), anti-CD45 allophycocyanin (APC ...
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bioRxiv - Systems Biology 2023Quote: ... Cell viability was determined using 7-AAD (BioLegend) or PI staining solution (Invitrogen eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were spiked with 7-AAD (BioLegend) to label non-viable cells ...
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bioRxiv - Cell Biology 2023Quote: ... Viability dye 7-AAD (1:250; BioLegend, 420403) was added for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... CD169/Siglec-1 (Clone 7-239, Biolegend 346002), or CD209/DC-SIGN (Clone 120507 ...
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bioRxiv - Cell Biology 2023Quote: ... Pecy-7 (Biolegend, clone HCD14, diluted 1/100); anti-CD16 ...
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bioRxiv - Microbiology 2023Quote: ... and 7 AAD (#420404; Biolegend 1:25 dilution) diluted in Annexin V binding buffer ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with 7-AAD (BioLegend, #420404) cellular DNA content marker (5 μl per sample ...
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bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...
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bioRxiv - Developmental Biology 2021Quote: TRA-1-60 staining was performed as previously described (4) and anti-SSEA1 antibody (Biolegend, catalog no. 125604) was used to quantify MEF reprogramming ...
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bioRxiv - Immunology 2019Quote: ... The reconstitution efficiency was analyzed by FACS 4 weeks after BMT using CD45.1-APC (A20; eBioscience) and CD45.2-PerCP (104; Biolegend) antibody.
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bioRxiv - Immunology 2021Quote: ... for 15 minutes on ice followed by staining with fluorescently conjugated Abs: anti-CD11a (clone M17/4- Biolegend), anti-KLRG1 (clone MAFA- Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... and stained intracellularly for 30 minutes in PBS with antibodies including: PE anti-mouse IL-4 antibody (Biolegend) or PE anti-mouse IFN-γ antibody (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2020Quote: ... The following directly conjugated antibodies were used in these experiments: CD4 Alexa Fluor 700 (Clone RM4-4, BioLegend), CD4 BUV737 (Clone GK1.5 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...