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Citations for BioLegend :
1901 - 1950 of 3284 citations for 7H Pyrrolo 2 3 d pyrimidine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Biophysics 2021Quote: ... Synomag-D+ DC were intradermally injected into each hind footpad immediately following Tag-It Violet™ (Violet+) cell tracking dye incorporation (Biolegend, San Diego, USA). MPI was done immediately following injection (day 0 ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 stimulation group (LN stim) with hashtag 4 (BioLegend, Cat# 155807); lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper ...
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bioRxiv - Genetics 2019Quote: ... anti-CD25-PE (8 µl) and anti-CD45RA-BV785 (4 µl, clone HI100, Cat. no. 304140, BioLegend), incubated at RT in the dark for at least 30 min. ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were cultured for 4 hours in the presence of PMA and Ionomycin (Cell Stimulation Cocktail, Biolegend) and Brefeldin A (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... stained for 30 minutes at 4℃ with the following antibody: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Immunology 2019Quote: ... anti-perforin PerCP-Cy5.5 (B-D48, BioLegend), anti-CD40L FITC (24-31 ...
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bioRxiv - Immunology 2021Quote: ... and FITC anti-human Granzyme B (BioLegend) staining.
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bioRxiv - Neuroscience 2021Quote: ... and b-FGF (160μg/ml; 571502, Biolegend). Experiments were conducted after 6 or 14 days of differentiation initiated by growing cells in differentiation media consisting of Advanced DMEM/F12 ...
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bioRxiv - Microbiology 2020Quote: ... anti-Granzyme B (2µg/ml, GB11; Biolegend), and IL-10 (2µg/ml ...
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bioRxiv - Immunology 2021Quote: ... granzyme b FITC (Biolegend 515403, 1:100), phospho-S6 APC Ser235/236 (Invitrogen 17-9007-42 ...
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bioRxiv - Immunology 2019Quote: ... anti-perforin PerCP-Cy5.5 (B-D48, BioLegend), anti-FasL PE (NOK-1 ...
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bioRxiv - Immunology 2021Quote: ... anti-Perforin-PE (clone B-D48) (BioLegend). T cells were gated based on the CD3 and CD8 expression ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Granzyme B-Pacific Blue (GB11; BioLegend, 515408), CD27-PE/Cy7 (LG.3A10 ...
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bioRxiv - Immunology 2022Quote: ... and Granzyme B (clone GB11; Biolegend; #515407) overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... stained with Total-Seq B antibodies (Biolegend) for CD11b ...
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bioRxiv - Immunology 2023Quote: ... CD49d-PE (9C10/MFR4.B, Biolegend 103706), KLRG1-PE (2F1/KLRG1 ...
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bioRxiv - Immunology 2023Quote: ... Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone: IP26, Biolegend), CD69-PerCP-eFluor-710(Clone ...
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bioRxiv - Cancer Biology 2023Quote: ... Granzyme B-PE/Dazzle 594 (BioLegend, 372216). Cell viability was assessed by using Fixable Viability Dye eFluor 780 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... F4/80 and Granzyme B antibodies (Biolegend) for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...