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Citations for BioLegend :
2001 - 2050 of 4015 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Biochemistry 2022Quote: ... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed with 1X PBS + 5% FBS + 0.1% sodium azide before being incubated for 20 minutes with Zombie Aqua viability stain (Biolegend) at room temperature ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each with PBS plus 0.05% Tween-20 and then blocked with 1x Assay Diluent A (BioLegend) for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... dissociated GL261 tumors (5 × 106 cells) were pre-incubated with 1.0 μg of TruStain fcX (anti-mouse CD16/32, clone 93; BioLegend) in 100 μL of FACs Buffer (DPBS with 1% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Immunology 2022Quote: Thawed 0.5×106 cells washed 3x at 400xg for 5 minutes at room temperature with 1x staining buffer (Biolegend) and kept on ice during the live dead staining using acridine orange and propidium dye to measure the cell viability before proceeding for 10x experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours of incubation splenocytes were treated for 5 hours with activation cocktail with brefeldin A1 (Biolegend, 423303) before staining for CD45 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... in duplicate for 2hours at 37°C prior to adding CD16a.NK-92 cells (PTA-6967, ATCC) (5 × 104 cells/well) for 5hours with brefeldin A (Biolegend), Golgi Stop (BDBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... The cells were differentiated into macrophages by incubation with 50 ng/ml macrophage colony–stimulating factor (M-CSF) (BioLegend) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 stimulation group (SPT stim) with hashtag 2 (BioLegend, Cat# 155803); spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper ...
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bioRxiv - Immunology 2021Quote: ... IFN-β and IFN-γ were determined using the LEGENDplex mouse type 1/2 interferon panel (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Physiology 2022Quote: ... mice were intravenously injected with 2 μg of Alexa Fluor 647 anti-mouse CD127 antibody (A7R34, BioLegend) and euthanized 5 minutes after injection ...
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Immunology 2022Quote: Cells were stained for flow cytometry using the following antibodies diluted in FACS Buffer (2% FBS, 0.01% Sodium Azide, PBS): BV711-anti-CD326 (EpCAM) (Clone:G8.8, BioLegend), BUV395-anti-CD45.2 (Clone:104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2) (Biolegend, San Diego CA) viability dye and blocked using 2% FBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed with 2% paraformaldehyde (Pierce, 28906) diluted in PBS or commercial fixation buffer (Biolegend, 420801) for 20 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 1–2 million cells/100 μl were incubated with the following antibodies: anti-CD11c PeCy7 (117318, Biolegend), anti-CD11b (101212 ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once and resuspended in 100μl blocking buffer (2%FBS and 1% FcX (BioLegend, 422302) in PBS ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used at a concentration of 1:100: CD14 (BioLegend, #123312, clone Sa14-2), CD86 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 2 hours after which cells were treated with 1x Brefeldin A (BioLegend; cat#: 420601) and incubated for a final 4 hours before analysis by flow cytometry for T cell activation.
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bioRxiv - Cell Biology 2020Quote: ... CD4 (1:50; Biolegend). After washing ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...