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Citations for BioLegend :
1951 - 2000 of 4015 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
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bioRxiv - Microbiology 2023Quote: ... A total of 2 × 105 donor-matched PBMCs or ToMCs were labeled with CFSE (BioLegend #423801), pre-treated with different inhibitors for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell surface staining was done for 30 minutes at 4 degrees with the following antibodies (all from BioLegend, San Diego, CA): CD45 (30-F11) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr at 4°C with the following antibodies: CD26 (DPP4)-fluorescein isothiocyanate (FITC) (Biolegend, 137806; 1:200), anti-mouse ICAM1-phycoerythrin (PE)/Cy7 (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...