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Citations for BioLegend :
1951 - 2000 of 10000+ citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 0.1% BSA/PBS 15 min on ice) and incubated with either FITC-anti-human CD3 (clone HIT3a; BioLegend, San Diego, CA) and PE-anti-human CD56/NCAM (HCD56 ...
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bioRxiv - Biophysics 2019Quote: ... siRNA mediated knockdown was further confirmed by imaging cells labelled with Alexa Fluor 488 anti-human FcƐRIɑ antibody AER-37 (CRA-1) (BioLegend Products, Singapore).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Cancer Biology 2019Quote: ... TDEs in 100 μl final volume of PBS were stained using the PE anti-human CD9 antibody (Clone HI9a) (BioLegend, CA) and incubated for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies used for flow cytometry were as follows: PE-conjugated mouse anti-human CD29 (Biolegend, San Diego, CA, USA; 303004), PE-conjugated mouse anti-human CD73 (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: 600 million cells from human tonsils were resuspended in Miltenyi magnetic-activated cell sorting (MACS) buffer and stained with biotinylated anti-human CD19 (clone: HIB19; BioLegend, #555411) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... and a cocktail of fluorescent antibodies: 1 µL each of anti-human CD3 (Brilliant Violet 421-conjugated, Biolegend 344834, clone SK7), anti-human CD69 (PerCP-eFluor710-conjugated ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
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bioRxiv - Genetics 2022Quote: ... Differentiation efficiency was measured using Fluorescence Activated Cell Sorting (FACS) following staining with an APC conjugated antibody to HLAG (APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK), in unmodified hTSC this was consistently between 40-70% ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were subsequently stained with Zombie Violet (1:400) viability dye and APC-Cy7 conjugated human anti-CD45 (BioLegend, clone: 2D1) for 45 minutes at 4°C protected from light ...
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bioRxiv - Immunology 2023Quote: ... intracellular FACS analysis use only) allophycocyanin (APC)/Cyanine7 or Brilliant Violet 421™ anti-human CD45 antibody (1:200, Biolegend, HI30), APC anti-human CD31 antibody (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Immunology 2023Quote: Cryopreserved PBMCs from blood collected 1 and 2 weeks after the H2-F boost were stained with anti-human monoclonal antibodies CD3 BV510 (OKT3, 1:400 dilution, BioLegend, RRID:AB_2561376), CD56 BV510 (HCD56 ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: Ligand-independent reporter cell activation was assessed by overnight stimulation with 6.25 µg mL-1 Ultra-LEAF™ Purified anti-human CD3 (OKT3, BioLegend, #317326). Cells were incubated in antibody-coated wells and TCR responses were measured by flow cytometric analysis of GFP expression after 16h ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenotyping of AML patient samples used a 1:50 dilution of the PE-Cy7-conjugated anti-human CD34 antibody (clone 581, Biolegend, UK). Samples were stained ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 million cells per condition were then stained with phycoerythrin-(PE)-conjugated anti-human CD147 antibody diluted 1:1000 in flow buffer (BioLegend #306211) for 20 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following antibodies on ice for 30 minutes: Alexa Fluor 647 anti-human CD3 (1:100 dilution, BioLegend, 300321), Alexa Fluor 488 anti-human CD4 (1:200 dilution ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human CD41/CD61 primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Immunology 2024Quote: Cells were pelleted and then resuspended in staining buffer and stained for 30 minutes on ice with 1 mg/mL AF700-mouse-anti-human CD45 (BioLegend; 368508) and PE-mouse anti-human CD235a in staining buffer ...
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bioRxiv - Bioengineering 2023Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2023Quote: ... we employed the LEGENDplex™ Human Inflammation Panel 1 (13-plex) flow cytometry-based assay kit (#740809, BioLegend, San Diego, CA, USA). This panel allowed us for simultaneous quantification of 13 human inflammatory cytokines and chemokines (IL-1β ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... using the LEGENDplex Human Angiogenesis Panel (BioLegend) bead-based immunoassay ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
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bioRxiv - Immunology 2019Quote: ... blocked with human TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse/human CD44-FITC (IM7, Biolegend 103005), FC ...
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bioRxiv - Bioengineering 2020Quote: ... and antibodies to human CD3 (HIT3a, BioLegend), CD45 (2D1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.3 μL human hashtag (HTO) antibody (Biolegend) was added to each sample (a different hashtag antibody was used for each sample/replicate ...
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bioRxiv - Neuroscience 2022Quote: ... then blocked with Human TruStain FcX (Biolegend). Cells were stained with FACS antibodes (Table 2 ...
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bioRxiv - Neuroscience 2021Quote: ... human CD45-FITC (BioLegend; dilution 1:25) and human CD31-V450 BD Biosciences ...