Labshake search
Citations for BioLegend :
1851 - 1900 of 10000+ citations for Anti CD25 SAP human Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
-
bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
-
bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences LTV-CD20) and sorted for CD20-expression using rituximab and a BV421-labeled anti-human secondary (Biolegend) as the staining reagent on a FACS instrument ...
-
bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
-
bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
-
bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
-
bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
-
bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and resuspended for 30 minutes on ice in 1/250 anti-human IgG1-Fc-FITC (clone HP6017, BioLegend) and 1/1000 anti-hemagglutinin (HA ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
-
bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following fluorochrome-conjugated antibodies: APC/Fire™ 750 anti-human CD324 (E-Cadherin) (Biolegend 324122), PE anti-human CD326 (EpCAM ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with cold PBS and then stained with a cocktail of fluorescently labeled antibodies including: anti-human CD3-PE-Cy7 (Clone: OKT3, Biolegend), Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone ...
-
bioRxiv - Immunology 2023Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
-
bioRxiv - Bioengineering 2023Quote: ... binding of T cell-secreted SiRPα monomer on T cell CD47 was assessed indirectly by staining with anti-human CD47 (CC2C6, Biolegend) at 4°C for 30min ...
-
bioRxiv - Microbiology 2023Quote: ... samples were stained with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with HBSS (Sigma Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... stained with a mixture of fluorochrome-conjugated antibodies (1:200 FITC anti-mouse/human CD11b M1/70, BioLegend, California, USA), (1:200 APC/Cyanine7 anti-mouse F4/80 ...
-
bioRxiv - Bioengineering 2023Quote: ... PE anti-mouse CD19 antibody (Catalog No. 115508) and PC anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend. MIK665 was from Selleck Chem ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by staining with Brilliant Violet (BV) 785 conjugated anti-human CD69 (10 μgꞏml−1) (Biolegend, Cat # 310932, clone FN50) for 30 min on ice ...
-
bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
-
bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... then Fc gamma receptors were blocked for 15 minutes with heat-inactivated human serum prepared in-house or anti-mouse CD16/32 (TruStain FcX, Biolegend) for human samples and mouse samples respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
-
bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human GPA primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
-
bioRxiv - Immunology 2023Quote: ... For comparison of resident vs non-resident populations tonsil samples were divided into CD69+ and CD69- subsamples using biotinylated anti-human CD69 antibody (FN40, Biolegend), and separation was performed using anti-Biotin Microbeads (Milenyi ...
-
bioRxiv - Immunology 2023Quote: ... and was analyzed with the DropArray (Curiox Biosystems) and the MAGPIX System (Luminex) or with LEGENDPlex Human Anti-Virus Response Panel (13-plex, BioLegend) and was analyzed with LSRII (BD ...
-
bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
-
bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
-
bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Immunology 2024Quote: ... Mouse anti-human HLA-E (clone 4D12) primary antibody (MBL Lifescience) and then secondary antibody goat anti-mouse conjugated with APC (BioLegend) were applied with staining buffer (DPBS ...
-
bioRxiv - Genomics 2023Quote: ... A subset of the hiPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
-
bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
-
bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
-
bioRxiv - Immunology 2024Quote: The following antibodies were used for the flow cytometric analysis of the cells: anti-human antibodies CD3-PE-Cy7 (SK7, BioLegend), TCRα/β-PE (IP26 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell suspensions were then labeled with lineage related antibodies: PE/Dazzle™ 594 anti-mouse/human CD11b (Biolegend 101256), APC anti-mouse Ly-6G/Ly-6C (Gr-1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
-
bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
-
bioRxiv - Cancer Biology 2024Quote: ... NK cells were analyzed by flow cytometry for expression of NKG2A using above mentioned protocol but with anti-human NKG2A PECy5 antibody (Biolegend).
-
bioRxiv - Cancer Biology 2021Quote: ... and incubated with Human FcX (BioLegend) to prevent non-specific antibody binding ...
-
bioRxiv - Immunology 2021Quote: ... FITC labeled human CD45RA antibody (BioLegend) and acquired with flow cytometer FACS Canto (BD).
-
bioRxiv - Immunology 2021Quote: ... APC labeled human CCR7 antibody (BioLegend), FITC labeled human CD45RA antibody (BioLegend ...
-
bioRxiv - Bioengineering 2020Quote: ... recombinant human IL-15 (570308, Biolegend), recombinant mouse scIL-12 (130-096 ...
-
bioRxiv - Neuroscience 2020Quote: ... human αSyn (4B12, 1:1000, BioLegend), pSer129-αSyn (pS129 ...
-
bioRxiv - Immunology 2021Quote: ... PerCP labeled human CD8+ antibody (BioLegend), APC labeled human CCR7 antibody (BioLegend) ...