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Citations for BioLegend :
2101 - 2150 of 3960 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated by negative selection using the EasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2019Quote: Immunofluorescence staining was performed in 96 well plates on seminiferous tubules with primary antibodies major histocompatibility complex class II [MHC Class II RT1D (BioLegend, Clone OX-17, 205401, 1:200)] ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Immunology 2022Quote: ... clones were screened by their loss of ability to produce IP-10 to recombinant IFNβ (Biolegend #581302).
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bioRxiv - Immunology 2021Quote: Timed-pregnant mice dams were intravenously injected with up to 10 μg of recombinant mouse cytokines (Biolegend) at gestation day 13.5 ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Microbiology 2021Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Bioengineering 2024Quote: T cells were activated by incubating PBMCs with anti-CD3 (OKT3, Tonbo/Cytek, San Diego, CA) and anti-CD28 (302934, Biolegend, San Diego, CA) antibodies in PBS at 37°C for 3 to 5 hours ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... viable T cells were enriched using the MojoSort Mouse CD3 T Cell Isolation Kit and Mouse Dead Cell Removal Kit (BioLegend, San Diego, CA).
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bioRxiv - Biophysics 2023Quote: ... in combination with a strong forwards and side scatter (for neutrophils) or a second cell-type specific surface marker (CD3 (Biolegend, cat. no. 300408) for T cells ...
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bioRxiv - Biochemistry 2021Quote: Quadruplicate samples of WT HEK293T and CCT5-FLAG tagged cells were lysed and purified using anti-FLAG affinity matrix (Biolegend cat no. 651501) using 20mM HEPES pH 7.5 and 100mM NaCl wash buffer and 200mM Glycine/150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human PVR/CD155 (Clone SKII.4, Biolegend), PE anti-human MICA (Clone 159227 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human ICAM-1/CD54 (Clone DX2, Biolegend), FITC anti-human Fas/CD95 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... BV421-conjugated mouse anti-human CD34 (Biolegend, CA, USA), PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Pacific Blue™ anti-human IgD (BioLegend, 348224). After 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD140a (PDGFRα) (Biolegend, clone 16A1; 1:50); anti-human CD309 (VEGFR2 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD56 (NCAM) (Biolegend, clone HCD56; 1:100); anti-human CD184 (CXCR4 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD184 (CXCR4) (Biolegend, clone 12G5; 1:100); anti-human CD117 (c-kit ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD140a (PDGFRα) (Biolegend, clone 16A1; 1:250); anti-human CD309 (KDR/VEG-FR2 ...
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bioRxiv - Immunology 2022Quote: PBMCs were firstly incubated with Human TruStain FcX (Biolegend) at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked using human TruStain FcX (BioLegend), and cells were then subsequently stained for surface markers anti-CD11c-FITC (Bu15 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human PD1-APC (Clone EH1202H7, Biolegend) all diluted 1:100 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human CD161-AF488 (Clone NKR-P1A, Biolegend), mouse anti-human IgD-BV421 (Clone IA6-2 ...