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Citations for BioLegend :
1801 - 1850 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Cancer Biology 2022Quote: ... is the complete list of antibodies used for experiments of T cell activation or exhaustion against primary AML: Anti-CD279 (PD-1) FITC (cat#329904 from Biolegend), CD366 (TIM-3 ...
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bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were cocultured with NALM6GL cells at indicate E/T ratio for 4 hours with the presence of 1x Brefeldin A (Biolegend). Cells were first stained with the Live/Dead-NearIR dye before fixed ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine stain was performed following restimulation of pmel-1 T cells as above with the addition of 1X Brefeldin A (Biolegend) and 1X Monensin (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... Pospho-GSK-3α/β and pospho-Akt were quantitated following 5 minutes of stimulation in transfected T cells fixed and permeabilized with fixation and permeabilization buffers (Biolegend) and stained with anti-pospho-GSK-3α/β (R&D systems ...
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bioRxiv - Cancer Biology 2023Quote: ... The frequency of CD8+ CD57+ population in freshly isolated T cells was assessed by using PE-conjugated anti-CD8 (clone RPA-T8, Biolegend) and PerCP-conjugated anti-CD57 (clone HNK- ...
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bioRxiv - Bioengineering 2023Quote: ... whereas binding of T cell-secreted A4 monomer on OT-I T cell CD47 was assessed indirectly by staining with anti-mouse CD47 (miap301, Biolegend) at 4°C for 30min.
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... reactivity of TCR-transduced PBMC-derived CD8+ T-cells was evaluated by flow analysis following anti-CD107a (BioLegend, Cat #328620) and anti-CD137 antibody (4-1BB ...
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bioRxiv - Immunology 2023Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... TCR reactivity was measured through detection of CD137 surface expression (PE-anti-human CD137, Biolegend) on CD8+ TCR transduced (mTCRB+ ...
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bioRxiv - Bioengineering 2021Quote: ... followed by surface-staining with monomeric GFP and anti-human CD45 AF700 (Biolegend clone HI30), CD19 PerCP-Cy5.5 (Biolegend clone SJ25C1) ...
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bioRxiv - Immunology 2022Quote: ... The following fluorochrome-coupled Abs were used for surface staining: anti-CD19 (Biolegend; clone HIB19), anti-CD38 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduction efficiency was measured by flow cytometry using surface staining for Thy1.1 (OX-70, Biolegend). For the IL-7 independency assay (Fig ...
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bioRxiv - Immunology 2020Quote: ... PBMC were washed in PBS and surface labelled with Zombie NIR fixable viability stain (BioLegend), CD4-PerCP-Cy5.5 mAb (clone 74-12-4 ...
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bioRxiv - Immunology 2023Quote: PBMCs were stained for the following surface markers: Panel 1) CCR7-FITC (Biolegend Cat. 353216), CD25-APC (Biolegend Cat ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Surface marker staining was performed for 30min after 15min blocking with human TruStain FcX(BioLegend). Cells were washed in FACS buffer(PBS+2% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... stained for surface markers (CD45 APC #103112, CD4 FITC #100406, Ly6G PE-Cy7 #127618, Biolegend), and then fixed in 1% paraformaldehyde in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated cells were incubated with labeled antibodies in Cell Staining Buffer (Biolegend). Dead cells were excluded based on staining with Live/Dead Fixable Viability Dye (FVD-eFluor780 ...
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bioRxiv - Cancer Biology 2021Quote: ... which consisted of anti-human Alexa Fluor® 700 anti-human CD3 (BioLegend, cat. 300324), Brilliant Violet 785™ anti-human CD4 (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... and stained extracellularly with anti-mouse CD3 phycoerithrin (PE)-Cy7 (clone 17A2, 1:100, Biolegend), anti-mouse CD4 Brilliant Violet 421 (clone GK1.5 ...
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bioRxiv - Immunology 2020Quote: ... and then stimulated with 10nM HyIL-6 or 0.1 μg/mL anti-CD3 (Biolegend #300438) and 20 ng/mL IL2 (Novartis #709421) ...
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bioRxiv - Immunology 2021Quote: ... Ultra-LEAF purified anti-CD3 (clone: 145-2C11) or IgG isotype control (clone: HTK888) (BioLegend) twice over 48 hr ...
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bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in 96-well plates coated with 1µg/ml anti-CD3 (BioLegend, Clone UCHT1) or control mIgG (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were activated on plates coated with anti-CD3 (1mg/ml, Biolegend, #317347, clone: OKT3) and anti-CD28 (1mg/ml ...
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bioRxiv - Immunology 2024Quote: ... Donor 1 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by human CD3 and CD8 (clone OKT3 and clone SK1, BioLegend, San Diego, CA) stain ...
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bioRxiv - Bioengineering 2021Quote: ... biotinylated Flex-T HLA-A*02:01 monomers (BioLegend), using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated Flex-T HLA-A*02:01 monomers (BioLegend), using a modified version of the commercial protocol ...
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bioRxiv - Immunology 2020Quote: ... PE/Cy7-conjugated anti-T-bet clone 4B10 (BioLegend) and FITC-conjugated anti-Eomes clone WD1928 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Brilliant Violet 605™ anti-T-bet (BioLegend #644817), Brilliant Violet 711™ anti-mouse/human CD11b (BioLegend #101241) ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human T-bet-APC (clone 4B10; BioLegend); mouse anti-human GATA3-PE (clone TWAJ ...
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bioRxiv - Immunology 2021Quote: ... and T-bet (4B10; 800x) were obtained from BioLegend. Streptavidin-conjugated antibodies were also obtained from BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-T-bet BV605 (Biolegend, Cat: 644817, 1:50), anti-RUNX3 PE (BD ...