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Citations for BioLegend :
2051 - 2100 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The cells were then dissociated in single cell suspension and seeded to Thy1.2 (CD90.2) antibody (#105302, Biolegend) coated Petri-dish to deplete astrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... and hashed cells from each mouse with mouse cell hashing antibodies (Totalseq™, BioLegend, San Diego, CA) before pooling and sorting ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... at 2:1 ratio for 1 h at 4C to ensure maximum staining quality before surface staining with CD20-FITC (2H7, Biolegend) for an additional 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-BV650, 1:200 Biolegend, #141723; CD45-BV786, 1:200, BD Biosciences #564225; CX3CR1-FITC, 1:100, Biolegend, #149019 ...
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bioRxiv - Immunology 2019Quote: ... The slides were blocked with 5% FBS in PBS for 1 hour at room temperature before surface staining with APC conjugated anti-CD45 (clone 30-F11; Biolegend) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were fixed for 15 min at 37°C after surface marker and viability dye staining using Fixation Buffer (Biolegend), followed by permeabilization for 1h at −20°C with True-Phos™ Perm Buffer (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with brefeldin A 2h at 37°C before surface stain with a mix of CD107a (LAMP1) [clone 1D4B (BioLegend)] and CD107b (Mac-3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2021Quote: ... we processed PBMC samples for scRNAseq with paired measurements of 192 surface proteins (CITE-seq, using TotalSeq reagents from Biolegend). The list of the antibodies included in the TotalSeq custom panel are detailed in Supplementary Data 12 ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Immunology 2022Quote: ... samples were thawed from −80°C and were stained for surface markers: Zombie UV fixable viability dye (Biolegend, Cat#423107), anti-CD3-BUV395 (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... PD-L1+ cells were sorted for on a BD FACS Aria III by PD-L1 surface staining (PD-L1 PE, clone 29E.2A3, BioLegend), then expanded in vitro in T-150 flasks in cRPMI for another week in the presence of 2 μg/ml puromycin before any functional studies or EV isolations.
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Physiology 2021Quote: ... after incubation with either anti-Ly6G-PE (clone 1A8) or anti-CD115-Biotin (clone AFS98, both BioLegend) antibodies followed by PE- and streptavidin-coated microbeads (Miltenyi) ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2020Quote: ... injected with 200 μL biotin-conjugated anti-CD45 mAb in PBS (15 μg/mL, Biolegend, clone 104) into the lateral tail vein ...
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bioRxiv - Immunology 2019Quote: ... anti-rat IgG1/2a/2b/2c-Biotin combined with Streptavidin-HRP Streptavidin-HRP (all BioLegend, London, UK).
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bioRxiv - Immunology 2020Quote: ... followed by intracellular staining with Ki-67-PE and t-bet-BV711 (Biolegend), Eomesodermin-eFluor660 and SAP-PE (eBiosciences) ...
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bioRxiv - Immunology 2023Quote: ... the T-EVs was incubated with human anti-IFNγ (clone MD-1) (Biolegend) for 15 min ...
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bioRxiv - Immunology 2024Quote: ... T-bet (4B10, BV421, Biolegend, or O4-46, PE-CF-594, BD Biosciences), PLZF (#6318100 ...
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set (eBiosciene) and afterwards intracellularly stained with CD3-AlexaFluor700 and Ki-67-BV711 (clone Ki-67, Biolegend). In-depth analysis of CD27−IgD− B cell phenotype was performed with Fixable viability stain 780 or aqua viability dye V510 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stored at 4° C o/n before surface staining for CD3 (eBioscience, clone SK7; not always included), CD161 (eBioscience, clone HP-3G10) and the Vα7.2 TCR (BioLegend, clone 3C10) to enrich for Vα7.2+ CD161high MAIT cells on a SONY MA900 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems ...
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bioRxiv - Immunology 2021Quote: TCRαβ-KO Jurkat E6.1 cells (a kind gift of Edward Jenkins) were transduced with 1G4 or A6 lentivirus and TCR expression was measured by staining for CD3 (clone: UCHT1; Biolegend) and TCRαβ (clone IP26 ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cell purity was assessed by flow cytometry using anti-CD4 Alexa 700 (eBioscience) and anti-CD3 Brilliant Violet (BV) 785 (BioLegend) antibody staining ...
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bioRxiv - Immunology 2020Quote: TCRαβ-KO Jurkat E6.1 cells (a kind gift of Edward Jenkins) were transduced with 1G4 or A6 lentivirus and TCR expression was measured by staining for CD3 (clone: UCHT1; Biolegend) and TCRαβ (clone IP26 ...
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bioRxiv - Immunology 2019Quote: ... Pre-enrichment for antigen presenting cells prior to sorting was done with biotinylated anti-CD3 (clone 145-2C11) and anti-CD19 (clone 6D5) both from Biolegend. Cell suspensions were incubated with antibodies for 10 minutes at room temperature at a dilution of 1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... clone HIT2), Lineage marker cocktail (BV510, CD3/14/16/19/20//56, clone OKT3/M5E2/3G8/HIB19/2H7/HCD56) (BioLegend) and PLXDC2 (PE/APC ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the tetramer plus CD3-eFluor 450 (1/100, 48-0032-82 eBioscience) and CD8-FITC (1/100, 300905 BioLegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with anti-CD3-FITC (clone 145-2C11; 1:100; eBioscience) and anti-TCRδ (clone GL3; 1:100; Biolegend) for 30 min on ice ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA, clone HI100, Biolegend; anti-CD45RO ...
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bioRxiv - Immunology 2023Quote: Staining for flow cytometry was done using the following anti-mouse mAbs: CD3 (17A2) conjugated to PE/Dazzle 594 (BioLegend), CD4 (RM4-5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2023Quote: T-IEL isolated from untreated Nr4a3-Tocky mice or mice that were injected with anti-CD3 (Clone 145-2C11) or IgG (both Biolegend) were stained with Viability dye-efluor780 (eBiosciences) ...