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Citations for BioLegend :
1651 - 1700 of 8191 citations for 6 BROMO 4 4 DIMETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... derived from OT-1 mice treated with either CATR or vehicle were restimulated with 1µg/ml OVA-peptide for 6 hours and Brefeldin A (Biolegend, 420601) for 4hr prior to analysis.
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bioRxiv - Molecular Biology 2024Quote: IL-6 KO mice with cast immobilization for 24 h were subjected to intraperitoneal administration of 400 ng of rIL-6 (575702, BioLegend) in saline or of saline alone ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... were independently stained with TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Biolegend, cat: 155835) according to the manufacturer’s instructions and with fluorophore-conjugated antibodies for cell sorting (anti-CD45.1-PB ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... filtered and resuspended in MEMα medium or FACS buffer containing 3 μg/ml DAPI (BioLegend). Cells were sorted on BD FACSAria1 or FACSAria2 machines and collected in 150 μl Arcturus PicoPure Extraction Buffer ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Immunology 2021Quote: ... αCD366 (Tim-3) BV711(RMT3-23) and αTIGIT PE-Dazzle594 (2G9) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with Brilliant Violet 421 anti-CD56 (clone HCD56, Biolegend, 3 μg/ml), APC anti-NKp30 (clone P30-15 ...
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bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Pathology 2023Quote: ... and resuspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Immunology 2024Quote: ... and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51, Biolegend, # 102116) per well ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2019Quote: Neutrophils were acutely depleted using intraperitoneal injection with 250 µg aGR1 in PBS (clone RB6-8C5, a gift from D. Portnoy, UC Berkeley, or from Biolegend), 16-24 hours before behavioral and flow cytometry experiments ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... All staining were performed in FACS buffer and dead cells were counterstained with 7-amino-actinomycin D (7-AAD, Biolegend). Before analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Cell Biology 2024Quote: ... was then carried out following the Chromium Next GEM Single Cell 3’ protocol for v3.1 reagents from 10X Genomics (CG000206 Rev D) and TotalSeq™-A Antibodies and Cell Hashing protocol from Biolegend (available online at https://www.biolegend.com/en-gb/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol) ...
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bioRxiv - Immunology 2019Quote: ... and anti-CD28 antibody (BioLegend, 2 μg/ml, clone 37.51) before use ...
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bioRxiv - Cancer Biology 2020Quote: ... CD18 (M18/2) and CD62L (MEL-14; all from Biolegend). In some experiments ...
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bioRxiv - Bioengineering 2021Quote: ... AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend) were used for staining of in vivo NP-treated alveolar and peritoneal macrophages ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend, 348210), 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-TNF-α BV785 and anti-IL-2 FITC (Biolegend) in PermWash buffer according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend). Conjugations were performed as previously described (65) ...
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... CD90-2 (alexa fluor 700 AM; clone 30-H12, BioLegend), B220 (CD45R ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was also neutralized (JES6-1A12, BioLegend, 5ug/mL). Cells were cultured on stimulation for 72-96h before analysis.
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bioRxiv - Cancer Biology 2021Quote: ... suspended in culture medium containing IL-2 (10ng/ml; BioLegend). Cells were directly stained with CFSE (Cayman Chemical ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE/Cy7 (clone MQ1-17H12, BioLegend, USA).
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bioRxiv - Immunology 2019Quote: ... anti-TNF-α Alexa488 and anti-IL-2 APC (Biolegend) with the Cytofix/CytoPerm staining kit (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... LEAF™ purified anti-CD18 (clone M18/2, 101409, BioLegend), LEAF™ Purified Rat IgG2a ...
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Epigenetic alterations underlie airway macrophage differentiation and phenotype during lung fibrosisbioRxiv - Immunology 2020Quote: ... and human CD206 APC-Cy7 (clone 15-2, BioLegend, USA). CD206+ cells were enriched in MACS LS magnetic separation column and MidiMACS™ magnet ...
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bioRxiv - Immunology 2020Quote: ... CD3 AF700 (clone OKT3, BioLegend, cat# 317340, 2:100 dilution); CD4 PerCP-Cy5.5 (clone OKT4 ...
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bioRxiv - Immunology 2020Quote: ... CD45RA BV510 (clone HI100, BioLegend, cat# 304142, dilution 2:100). After cell surface staining ...
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bioRxiv - Immunology 2021Quote: ... or biotin anti-mouse IL-2 (Clone: JES6-5H4, BioLegend) SA-HRP (Jackson immunoresearch ...
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bioRxiv - Immunology 2021Quote: ... or purified anti-mouse IL-2 (Clone: ES6-1A12, BioLegend). Plates were then incubated with blocking solution containing 10% FBS in PBST (0.05% Tween-20) ...
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bioRxiv - Cell Biology 2022Quote: ... treated with streptavidin-labeled allophycocyanin (APC) (2 μg/mL BioLegend) for 15 min on ice ...
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bioRxiv - Immunology 2022Quote: ... anti-TRAV1-2 BV510 (clone 3C10; Biolegend, San Diego, CA), anti-Pan-gδ PE-Vio770 (clone 11f2 ...
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bioRxiv - Immunology 2022Quote: ... with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend) in the presence or absence of a mouse anti-human PD1 (2 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and 2 ng/mL recombinant mouse IL-7 (577802; BioLegend) for 28 days ...