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Citations for BioLegend :
1551 - 1600 of 7633 citations for 6 BROMO 4 4 DIMETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... followed by Anti- ERK1/2 (pT202/pY204)-Alexaflour647 (Biolegend) staining at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD138 (281–2) (purchased from BD Biosciences, eBiosciences, BioLegend, Vector Laboratories Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... and (2) anti-NESTIN (clone 10C2; #656802, BioLegend, USA) and anti-EGFRvIII (clone RM419 ...
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bioRxiv - Immunology 2023Quote: ... and OX40 PE-Cy7 (2:100; Biolegend Cat#350012). All samples were acquired on ZE5 cell analyzer (Bio-rad laboratories ...
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bioRxiv - Immunology 2023Quote: ... anti-human IgD-PerCP/Cy5.5 (clone IA6-2, BioLegend), anti-human IgD-Alexa Fluor 700 (clone IA6-2 ...
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bioRxiv - Immunology 2023Quote: ... in Panel 2: CD3-FITC (Biolegend, UCHT1, Cat. 300406), CD4-PE-Cy7 (Biolegend ...
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bioRxiv - Systems Biology 2024Quote: ... and 30 U/ml mouse recombinant IL-2 (Biolegend).
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bioRxiv - Cell Biology 2023Quote: ... macrophages or granulocytes (CD14, Sa14-2-APC, #123311, BioLegend and CD11b ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... were independently stained with TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Biolegend, cat: 155835) according to the manufacturer’s instructions and with fluorophore-conjugated antibodies for cell sorting (anti-CD45.1-PB ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse CD86 (GL1) and anti-mouse CD40 (Clone 3/23) were all from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... filtered and resuspended in MEMα medium or FACS buffer containing 3 μg/ml DAPI (BioLegend). Cells were sorted on BD FACSAria1 or FACSAria2 machines and collected in 150 μl Arcturus PicoPure Extraction Buffer ...
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bioRxiv - Immunology 2021Quote: ... 3) mouse anti-human CD196 prelabeled with Alexa Fluor 488 (CCR6 marker; clone G034E3, BioLegend) and 4 ...
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bioRxiv - Immunology 2021Quote: ... αCD366 (Tim-3) BV711(RMT3-23) and αTIGIT PE-Dazzle594 (2G9) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: Macrophages (~3 x 106) were stained with anti-F4/80 (rat monoclonal FITC-conjugated, BioLegend) in a 1:200 dilution in FACS buffer for 20 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with Brilliant Violet 421 anti-CD56 (clone HCD56, Biolegend, 3 μg/ml), APC anti-NKp30 (clone P30-15 ...
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bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
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bioRxiv - Pathology 2023Quote: ... and resuspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Pathology 2023Quote: ... and suspended in 3 ml red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 90 s to remove the remaining red blood cells and diluted in 9 mL PBS after incubation ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2019Quote: Neutrophils were acutely depleted using intraperitoneal injection with 250 µg aGR1 in PBS (clone RB6-8C5, a gift from D. Portnoy, UC Berkeley, or from Biolegend), 16-24 hours before behavioral and flow cytometry experiments ...
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... All staining were performed in FACS buffer and dead cells were counterstained with 7-amino-actinomycin D (7-AAD, Biolegend). Before analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Cell Biology 2023Quote: ... was then carried out following the Chromium Next GEM Single Cell 3’ protocol for v3.1 reagents from 10X Genomics (CG000206 Rev D) and TotalSeq™-A Antibodies and Cell Hashing protocol from Biolegend (available online at https://www.biolegend.com/en-gb/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol) ...
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bioRxiv - Immunology 2019Quote: ... and anti-CD28 antibody (BioLegend, 2 μg/ml, clone 37.51) before use ...
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bioRxiv - Cancer Biology 2020Quote: ... CD18 (M18/2) and CD62L (MEL-14; all from Biolegend). In some experiments ...
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bioRxiv - Bioengineering 2021Quote: ... AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend) were used for staining of in vivo NP-treated alveolar and peritoneal macrophages ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend, 348210), 0.0032 μg biotinylated SARS-CoV-2 BA.1 protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-TNF-α BV785 and anti-IL-2 FITC (Biolegend) in PermWash buffer according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 2 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 2 μL PE/Cyanine7 anti-human IgD antibody(BioLegend ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend). Conjugations were performed as previously described (65) ...
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... CD90-2 (alexa fluor 700 AM; clone 30-H12, BioLegend), B220 (CD45R ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was also neutralized (JES6-1A12, BioLegend, 5ug/mL). Cells were cultured on stimulation for 72-96h before analysis.
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bioRxiv - Cancer Biology 2021Quote: ... suspended in culture medium containing IL-2 (10ng/ml; BioLegend). Cells were directly stained with CFSE (Cayman Chemical ...