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Citations for BioLegend :
1501 - 1550 of 2155 citations for 5 acetyl 2 methylfuran 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated in the antibody mix (2 μg/ml rat anti-CD326 (BioLegend 312502), 1 μg/ml rabbit anti-RAD51-AF647 (Bioacademia 70-012) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then stained with a cocktail of cell surface antibodies (Biolegend, Table 2) in 0.5%BSA/PBS and analyzed on a LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... Each tube of cells then received 2 µL of Fc block (BioLegend, San Diego, CA), 10 µL of Brilliant Stain Buffer (Becton Dickinson) ...
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bioRxiv - Immunology 2023Quote: ... hydrogel beads were stained with anti-CD138 PE (1:80 dilution, clone 281-2, BioLegend), anti-FLAG PE-Cy7 (1:200 dilution ...
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bioRxiv - Microbiology 2023Quote: ... we administered 2 μg of APC/Fire750-labeled anti-CD45 mAb (Biolegend, clone 30-F11) in mice anesthetized with an overdose of ketamine and xylazine ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Biophysics 2023Quote: ... and 6.25 μg/mL activation antibodies against Integrin β2 (clone m24 and LFA1/2, Biolegend) were added in cell culture medium at the start of the culture.
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bioRxiv - Cancer Biology 2023Quote: ... and Jagged1/2 blocking antibodies and IgG isotype control (Biolegend, cat#s 130902, 131001, 400902) were used at 20 µM/ml.
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% mercaptoethanol) supplemented with 2 μL Cell Activation Cocktail with Brefeldin A (Biolegend, #423304) and incubated for 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The cells were then incubated overnight with rat anti-CD14 IgG (clone Sa14-2; BioLegend) and goat anti-GST IgG (Rockland ...
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bioRxiv - Biochemistry 2024Quote: ... were coated overnight with purified anti-IL2 antibody (clone JES6-1A12, Biolegend, 2 μg/mL) diluted in PBS (50 μL/well) ...
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bioRxiv - Immunology 2024Quote: ... then floated on 2 μg/ml anti-Vγ5 and anti-CCR6 (BioLegend, San Diego, CA) for 1 hour at 37°C with 5.0% CO2 ...
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bioRxiv - Immunology 2024Quote: For in vivo intravascular labelling we injected intravenously 3µg of anti-CD8β.2 FITC (Biolegend). 3 to 5 min post injection ...
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated with 3µg/ml anti-CD3 and 3 µg/ml of anti-CD28 antibody (Biolegend clones 145-2C11 and 37.51) for 2 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... anesthetized mice (MMF i.p.) were administered with 3 µg of fluorescent-labeled anti-mouse Ly6G antibody (Alexa Fluor® 488, clone: 1A8, Biolegend, California, USA) fluorescent-labeled antibodies via i.v ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were incubated for 1 hour in 60 µL of staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 µL of anti-BrdU-FITC monoclonal antibody (Biolegend 364104, clone 3D4). The samples were subsequently washed with 1 mL of 1% BSA and 0.5% Tween-20 in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... centrifuged in a table top centrifuge at 1,500rpm for 5 minutes and resuspended in 200µl staining solution containing conjugated antibodies Ep-CAM-APC (#118213 Biolegend) and Brilliant violet 785-CD45 (#103149 Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... Red blood cells were lysed on ice for 5 min by using 1x RBC Lysis Buffer (BioLegend, 420301). Bone marrow cells (3×106 per well ...
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bioRxiv - Immunology 2021Quote: ... The PBMC samples were stained with 5 µl of anti-mouse CD3e-PerCP/cy5.5 (Biolegend, USA, cat#100327), NK1.1-PE (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... then ILC2s were diluted 1:5 with PBS containing 1 μg/ml DAPI and Precision Count beads (BioLegend) were added to each sample ...
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bioRxiv - Immunology 2022Quote: ... 5×105-1×106 cells were stained with antibodies to CD45 (D058-1283, Biolegend, San Diego, CSA, USA), CD3 (clone SP34 ...
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bioRxiv - Immunology 2022Quote: ... 5 min prior to euthanasia each mouse was injected retro-orbitally with 5ug of anti-CD45.2-APC (Biolegend) diluted in 200 ul of PBS.
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bioRxiv - Immunology 2022Quote: ... 5x105 cells were plated per well of a 48-well plate coated with CD3 (5 µg/ml) (Biolegend) and cultured in regular T cell media (RPMI 1640 supplemented with 10% heat inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...
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bioRxiv - Immunology 2021Quote: ... Murine monoclonal FITC anti-human CD11b (activated) antibody (IgG1, clone CBRM1/5) was from Biolegend (San Diego, CA). Goat anti-mouse (GαM ...
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bioRxiv - Microbiology 2021Quote: ... The samples were permeabilized for 5 min using Intracellular Staining Perm wash buffer (BioLegend, San Diego, CA, USA). After blocking with 1% BSA in PBST for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2020Quote: ... 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of IL-10-BV421 antibody (Biolegend, 501421) was added for an additional 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL αCD40 (BioLegend), 5 ng/ml TGFβ (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... CA) and phycoerythrin/Cy7 (PECy7)-conjugated anti-mouse CD71 (clone RI7217; 5 μg/mL; BioLegend, San Diego, CA) antibodies were added ...
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bioRxiv - Immunology 2022Quote: ... were injected into the microfluidic circulation in HBSS with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were washed with 1xPBS and blocked with 5% mouse Fc blocking reagent (Biolegend, San Diego, CA) in FACS buffer (1% FBS and 1 mM EDTA in PBS ...
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bioRxiv - Immunology 2024Quote: For immunophenotyping of HD’s and PLWH’s neutrophils the following antibodies were used: PerCPCy5-5-CD15 (HI98, Biolegend #301922), PE-LOX-1 (15C4 ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Neuroscience 2023Quote: ... C57Bl/6J mice were injected intravenously with 5 µg of AF488 conjugated to anti-CD4 antibody (100529; Biolegend) and 5 µg AlexaFluor647 conjugated to anti-CD8 antibody (100724 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell suspensions were centrifuged at 1500x rpm for 5 minutes and resuspended in RBC lysis buffer (Biolegend) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Counterstains in PBS were performed directly after secondary antibody incubation (DAPI 1µg/mL 5 minutes, Phalloidin (Biolegend#424203) 5 units/mL) ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Genomics 2024Quote: ... Cells were collected by centrifugation at 300 g for 5 minutes and washed with cell staining buffer (Biolegend). Cells were incubated with FcX (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... One to two million cells were stained with 5 ug/ml of biotinylated anti-CD16 (clone 3G8; BioLegend), anti-CD3 (clone UCHT1 ...
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bioRxiv - Neuroscience 2024Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7-anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...