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Citations for BioLegend :
1601 - 1650 of 2155 citations for 5 acetyl 2 methylfuran 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... the cell culture media was replaced with complete Huh-7.5 media with 5 μg/mL BFA (Brefeldin A, Biolegend) or equal volumes of DMSO (vehicle control ...
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... NK cells were pre-incubated for 20 minutes with anti-CD56 Alexa Fluor 647 (HCD56, Biolegend, 5 μg/ml). For granule polarization assays ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Biochemistry 2022Quote: ... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... and kept the remaining wells unstimulated by adding only an equal volume of 2X brefeldin (5 ug/mL, BioLegend). We incubated plates at 37°C for 4 hours.
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each with PBS plus 0.05% Tween-20 and then blocked with 1x Assay Diluent A (BioLegend) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and 50 μl of conditioned complete medium containing primary antibody at concentrations of 5 μg/ml for CD59 (Biolegend) or 10 μg/ml Alexa Fluor 488–linked transferrin (Molecular Probes ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Immunology 2022Quote: Thawed 0.5×106 cells washed 3x at 400xg for 5 minutes at room temperature with 1x staining buffer (Biolegend) and kept on ice during the live dead staining using acridine orange and propidium dye to measure the cell viability before proceeding for 10x experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was filtered through a 40 μm filter and then placed in 5 mL RBC lysis buffer (BioLegend) for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were spun down and resuspended in 5 mL of 1x Red Blood Cell Lysis buffer (Biolegend) and incubated on ice for 5 minutes with occasional swirl ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours of incubation splenocytes were treated for 5 hours with activation cocktail with brefeldin A1 (Biolegend, 423303) before staining for CD45 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... in duplicate for 2hours at 37°C prior to adding CD16a.NK-92 cells (PTA-6967, ATCC) (5 × 104 cells/well) for 5hours with brefeldin A (Biolegend), Golgi Stop (BDBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC, clone 53-6.7, 1:400, BioLegend; B220 PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were removed by incubating the cells with 5 ml ammonium-chloride-potassium (ACK) lysis buffer (Biolegend) for 5 min at room temperature followed by adding 10 ml RPMI supplemented with 3% FBS ...
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bioRxiv - Immunology 2024Quote: ... then resuspended in 200 µL of binding buffer and stained with 5 µL of 7-AAD (Cat#: 420403, Biolegend) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... + 5 ml FBS + 2mM Ethylenediaminetetraacetic acid [EDTA]) with Human TruStain FcX™ Fc Receptor Blocking Solution (1:20; BioLegend) for 15 minutes at room temperature in foil ...
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bioRxiv - Immunology 2024Quote: ... and individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Cancer Biology 2024Quote: ... centrifuged at 500g for 5 minutes and resuspended in a solution of 1% bovine ser albumin (BSA) (Biolegend, 644710) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: Bulk cultured T cells were stimulated with 5 µM peptides for 5hr in the presence of brefeldin A (Biolegend). The cells were collected and stained with specific antibodies against surface markers ...
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bioRxiv - Bioengineering 2024Quote: The T cells generated under standard conditions were stained on day 5 of culture with the antibodies Thy1.1-APC (Biolegend) and pan-TCR-α/β-PE (Beckman Coulter) ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...