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Citations for BioLegend :
1451 - 1500 of 2923 citations for PCSK9 Human V474I G670E HEK293 His HA Avi since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... IFN-γ (LegendPlex Human anti-virus response panel, Biolegend #740390) and IL-8 ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX™ (Fc Receptor Blocking Solution, 422302, BioLegend) was used at 1:100 dilution for blocking nonspecific binding ...
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bioRxiv - Immunology 2024Quote: ... and PE-Cyanine7 anti-human CD8 (Clone: SK1; BioLegend®) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-human CD3 antibody (Clone HIT3a, BioLegend Cat. #300307) to assess the surface-level expression of the transduced TCR ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human EPCAM–APC or EPCAM–PE (1:100; BioLegend), anti-human CD117–FITC or CD117–APC-Cy7 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and after Fc receptor blocking with Human TruStain FcX (Biolegend), cells were stained with the following antibodies in FACS buffer (PBS with 2% fetal calf serum ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human CD20 (BioLegend, San Diego, CA, USA, cat.: 302326), anti-human CD27 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: Cells were blocked with Human TruStain FcX (BioLegend, Cat# 422302) in fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2023Quote: ... PE/Cyanine7 anti-human CD69 (1:50 dilution, BioLegend, 310911), Brilliant Violet 421 anti-human CD134 (OX40 ...
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bioRxiv - Genomics 2024Quote: ... followed by APC anti-human CD195 (CCR5) (clone J418F1, BioLegend) or 5 µl of the corresponding isotype control staining for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and cells were preincubated in Human TruStain FcX™(Biolegend) and True-Stain Monocyte Blocker™ (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... PerCP-Cy5.5 anti-mouse/human CD11b (1:200, #101227, BioLegend), FITC anti-mouse MHCII (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Human TruStain FcX (BioLegend, Cat#422301; 5 μl/sample) for 10 min ...
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bioRxiv - Immunology 2023Quote: ... anti-human HLA-A,B,C (clone W6/32, BioLegend), anti-human CCR7 (clone 3D12 ...
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bioRxiv - Biophysics 2024Quote: ... antibodies Alexa Fluor 700 anti-human CD63 (BioLegend, cat. 353024) and PE anti-human CD9 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa 647 anti-human CD45 (1:400; HI30 Biolegend 304056), rabbit anti-human/mouse cleaved-caspase 3 (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The protein levels of overexpressed PRT6-WT and PRT6-136H+161V were determined by Western blot using anti-HA antibody (16B12, BioLegend).
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bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Neuroscience 2020Quote: HA-tagged-ribosome immunoprecipitation in the cerebellum of Gfap-RiboTag mice was performed as it was previously described (Ceolin et al., 2017) using anti-HA antibody (5μl/sample; Biolegend; Cat#901502) and magnetic beads (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The chambers were then incubated with 10 μg/ml purified Anti-HA 11 Epitope tag antibody (BioLegend, San Diego, CA, USA) in BIB at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked using DMEM/FBS media for 1 hour at RT followed by incubation with 1:1000 dilution mouse anti-HA (Biolegend, 901514) and rabbit anti-PCNA (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and approximately 106 cells were resuspended in FACS buffer containing an anti-HA AF488 conjugate (1:1000, BioLegend, 901509) and an eFluor 780 fixable viability dye (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in Cyto-Fast Wash Buffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue homogenates were centrifuged to remove tissue debris before being incubated with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
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bioRxiv - Immunology 2021Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD123 BV510 (1:100, clone 6H6, BioLegend, cat # 306022), Anti-human CD34 BV510 (1:100 ...