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Citations for BioLegend :
1351 - 1400 of 3135 citations for PCSK9 Human V474I G670E HEK293 His HA Avi since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-mouse/human CD44 (IM7, Biolegend, 103026, 1:200), anti-mouse CD8a (53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... and anti-human IL-4 (2μg/ml; Biolegend, Inc).
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bioRxiv - Immunology 2024Quote: ... and FITC-conjugated anti-human CD45RO (304204, BioLegend, USA) at 4 °C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used in the study: mouse monoclonal antibody to HA (16B12) from Biolegend (San Diego, CA); rabbit polyclonal to GFP (A10262) ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... The expression level of specific proteins was detected by immunoblot analysis with the following antibodies: HA (BioLegend, MMS-101R), V5 (Thermo Fisher Scientific R96025) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was transferred to a new tube with 30 µl HA.11 antibody-conjugated agarose beads (Biolegend, 900801) added to each sample ...
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bioRxiv - Biochemistry 2023Quote: ... monoclonal mouse anti-HA 11 epitope (catalogue number: 901502) and mouse anti-Tubulinβ3 (catalogue number: 657402) were from BioLegend. Polyclonal rabbit anti-IKKα/β (H-470) ...
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was transferred to a new tube with 30 µl HA.11 antibody conjugated agarose beads (Biolegend, 900801) added to each sample ...
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bioRxiv - Cell Biology 2024Quote: ... The sliced sections were incubated with an anti-HA monoclonal antibody (1:1000; BioLegend, San Diego, CA, USA, #901514), followed by anti-mouse Alex Fluor 488-conjugated immunoglobulin (1:100 ...
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bioRxiv - Microbiology 2024Quote: Western and immunofluorescence assays (IFA) were probed using the following antibodies: mouse monoclonal antibody (mAb) HA.11 (Biolegend; 901515), rabbit polyclonal antibody (pAb ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-human CD206 and PE/Cy7 anti-human Interleukin-10 (IL-10) were used for analysis (all from BioLegend, San Diego, CA). The samples were run on an LSR Fortessa cell analyzer from BD (Becton Dickinson ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-human CD247 (CD3ζ) mAb (clone 6B10.2) and mouse anti-human EGFR antibody (clone AY13) were purchased from Biolegend (San Diego, CA, USA). Anti-human CD32 mAb (clone 8.26 ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... For surface antigen staining cells were incubated for 20 min with FITC-labelled anti-human CD44 (eBioscience, clone IM7) and PE-labelled anti-human CD82 (Biolegend, clone ASL-24) antibodies and washed twice with 1% BSA staining solution ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation (VMAT2, 1:50, EB06558, Everest Biotech, Ramona, USA, HA-tag: 1:50, 682404, Biolegend, San Diego, USA) was performed for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Equivalent surface expression level was verified using an Alexa Fluor 488-conjugated anti-HA-tag mAb (clone 16B12, 1:1000 Biolegend). Flow cytometry data were analyzed using FlowJo V10 software.
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The protein levels of overexpressed PRT6-WT and PRT6-136H+161V were determined by Western blot using anti-HA antibody (16B12, BioLegend).
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
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bioRxiv - Plant Biology 2024Quote: ... which were then probed with the following primary and secondary antibodies with the indicated dilutions: αHemagglutinin (HA, 1:2000, BioLegend), αGFP (1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: ... The PE anti-human CD274/PD-L1 (Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b ...
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bioRxiv - Cancer Biology 2021Quote: ... APC anti-human CD3 antibody (clone HIT3a, catalog #300319, BioLegend). The 10x library was sequenced on an Illumina HiSeq 2500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... APC-Fire 750 anti-human CD20 (302357, clone 2H7, BioLegend), and APC-H7 anti-human CD3 (560275 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Human TruStain FcX™ (Biolegend), and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone ...
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bioRxiv - Immunology 2021Quote: ... and APC-conjugated mouse anti-human IgD (Biolegend, CA, USA). To determine the purity of CB-SC ...
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bioRxiv - Immunology 2021Quote: ... and Fc blocked for 10 minutes (Human TruStain FcX, Biolegend), and incubated with a cocktail containing CD3-PE (SP34 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD309 (VEGFR2) (Biolegend, clone 7D4-6; 1:50); anti-human CD56 (NCAM ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/ml recombinant human M-CSF (BioLegend #574806) for 6 days to obtain monocytes derived macrophages ...
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bioRxiv - Immunology 2021Quote: ... 5ng/ml or 50ng/ml recombinant human G-CSF (BioLegend) or mouse G-CSF (BioLegend) ...
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bioRxiv - Bioengineering 2019Quote: Devices stained with Alexa Fluor 647 anti-human CD31 (BioLegend) and Alexa Fluor 594 Phalloidin (ThermoFisher ...