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Citations for BioLegend :
101 - 150 of 6571 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Cell Biology 2022Quote: ... eYFP+ and bulk epithelial cells were incubated with anti-mouse Hashtag antibodies (BioLegend Cat #155831,155833, 155835, 155837) before sorting ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of mouse cell-surface molecules: ① Purchased from Biolegend(USA): Anti-CD45-BV510(clone 30-F11);Anti-Ly-6G/Ly-6c(Gr-1)-PerCP(clone RB6-8C5);Anti-CD4-BV421(clone GK1.5);Anti-F4/80-FITC(clone BM8);Anti-CD8a-PE(clone 53-6.7) ...
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bioRxiv - Immunology 2023Quote: ... Soluble peptide:IAg7 proteins were produced using S2 insect cells as previously described and made into tetramers by conjugating the soluble peptide:IAg7 molecules with BV421 (BioLegend), PE ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were treated with recombinant TGF-β1 (580702, BioLegend®), as specified in figure legends ...
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bioRxiv - Microbiology 2021Quote: ... HRP tagged anti-mouse (Biolegend 405306), Anti-rabbit Alexa Fluor 488 (Invitrogen A27206) ...
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bioRxiv - Microbiology 2023Quote: ... HRP tagged anti-rabbit (Biolegend 406401), HRP tagged anti-mouse (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... HRP tagged anti-mouse (Biolegend, 405306), anti-rabbit Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were incubated with autologous activated platelets (1:10 ratio) (65) for 2.5h and human recombinant CCL28 (50 nM) (BioLegend), the CCR3 antagonist SB328437 (10 mM ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IFNα (Biolegend), TNFα (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... PE rat anti-epithelial cadherin (ECAD, 1:250, 147304, BioLegend), BV421 rat anti-epithelial cell adhesion molecule (EPCAM ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... recombinant IFNα2 (#592702) and recombinant IFNγ (#570202) were obtained from BioLegend. PNGase F (#P0704S ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... Incorporation of BrdU in proliferating intestinal epithelial cells was detected using an anti-BrdU antibody (Biolegend, 1:100 dilution) after deparaffinization of the tissues ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2021Quote: ... and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M; 1:1000, Biolegend, 637314) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2021Quote: Recombinant mouse GCSF (BioLegend) was injected intraperitoneally (125μg/mouse ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine SCF (BioLegend), recombinant murine G-CSF (BioLegend) ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant mouse IFNβ (BioLegend), PALA (Open Chemical Repository of the Developmental Therapeutics Program NCI/NIH) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the epithelial layer were sorted gating on single positive for CD326-PE/Cy7 (BioLegend, clone DECMA-1, #147310) and CD104-AF647 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... B16-F1 and A375 cells were treated using carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Molecular Biology 2023Quote: ... Raw 264.7 cells were cultured with 100 ng/mL recombinant mouse IFNβ1 (BioLegend). After 24 h ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Immunology 2021Quote: ... adipose and/or splenic iNKT cell samples were first tagged by TotalSeq-A Mouse hashtag antibodies (Biolegend) and then pooled for sequencing ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with Human TruStain FcX (Biolegend), surface-stained with CD16-eFluor450 ...
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bioRxiv - Microbiology 2023Quote: ... the LEGENDScreen™ Human Cell PE Kit (Biolegend) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... the tumor organoids were treated with the immune checkpoint inhibitor Nivolumab (Selleckchem, Cat#A2002) and the Ultra-LEAF Purified Human IgG4 Isotype Control Recombinant Antibody (Biolegend, Cat#403701) at a concentration of 10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... and tetramerized using fluorescently tagged streptavidin from Biolegend. 5,6-Dimethylxanthenone-4-acetic Acid (DMXAA ...
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bioRxiv - Immunology 2021Quote: ... metal-tagged antibodies including pan-keratin (C11, Biolegend), BDCA2 (Polyclonal ...
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bioRxiv - Immunology 2019Quote: ... Each cell sample was incubated with an antibody cocktail for epithelial surface marker expression (see Supplemental Information) and Live/Dead™ Fixable Zombie Blue UV Cell Stain (Biolegend) for 30 minutes before rinsing in 1ml base media and centrifuging at 300g for 5 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Slides were incubated in blocking buffer for 30 minutes at RT prior to staining for epithelial cells by anti-mouse IgG2a CD326/Ep-CAM conjugated to Alexa-Fluor 488 (Biolegend, 118210) for 1 hour at RT ...