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Citations for BioLegend :
451 - 500 of 6571 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 10 ng/mL recombinant mouse GM-CSF (BioLegend, 576302). Cells were passaged every 2 days at sub-confluent concentration of 1×105 cells/mL ...
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bioRxiv - Immunology 2021Quote: ... eventually supplemented with 10 ng.mL-1 recombinant XCL1 (758002, BioLegend), 10 ng.mL-1 in house-generated XCL1(CC3) ...
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bioRxiv - Genomics 2022Quote: ... 50 ng/mL of recombinant IL-4 (BioLegend, Cat# 574302) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 1 ng/mL of recombinant IL-2 (BioLegend, Cat#575402) and 1 μg /mL of soluble anti-CD28 (Clone ...
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bioRxiv - Immunology 2022Quote: ... and 2 ng/mL recombinant mouse IL-7 (577802; BioLegend) for 28 days ...
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bioRxiv - Bioengineering 2023Quote: ... which comprised 1.5 μg of mouse recombinant IL-2 (Biolegend) and 50 μg of anti-IL-2 monoclonal antibodies (S4B6 ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/mL of recombinant mouse IL-4 (BioLegend) diluted in PBS was added to wells cultured with the cytokine ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10 ng/mL recombinant mouse GM-CSF (Biolegend). Cells were incubated at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Recombinant murine IFN-β1 (Cat# 581304) was purchased from BioLegend. AKT inhibitor VIII (Cat# A6730) ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/ml of recombinant IL-4 (Cat# 574302, BioLegend), and 10 ng/ml of recombinant IL-13 (Cat# 575902 ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 647 anti-human human CD3 Antibody (BioLegend, 300416), Biotin anti-human CD3 Antibody (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... binding of T cell-secreted SiRPα monomer on T cell CD47 was assessed indirectly by staining with anti-human CD47 (CC2C6, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2023Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Human α-synuclein in cell culture media was detected using LEGEND MAX ELISA kit (BioLegend). Total cholesterol levels were measured using the Amplex Red Cholesterol Assay Kit (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... B cells were also stained with following anti-human antibodies: anti-IgD-BV421 (Biolegend, 348226), anti-CD27-FITC (BD biosciences ...
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bioRxiv - Systems Biology 2020Quote: ... CD4+ were purified by negative selection using magnetic human CD4+ T cells nanobeads (MojoSort, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed in PBS and incubated for 1hour with secondary anti-human FITC (Biolegend) at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Up to 60,000 sorted live synovial cells were pre-incubated with Human TruStain FcX (BioLegend) in Cell Staining Buffer (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended at 106 cells/mL and then treated with Human TruStain FcX blocker (BioLegend 422302). Cell sorting was carried out in azide-free buffers ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with 40 ng/ml human IL-10 (BioLegend, San Diego, CA, USA) for 2 h at 4°C and cells were collected for flow cytometric analysis.
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bioRxiv - Cancer Biology 2019Quote: ... the cells were fixed and stained by anti-human mAb FOXP3-PE antibody (BioLegend, CA) and anti-human mAb CD4–FITC clone RPA-T4 (BioLegend ...
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bioRxiv - Genetics 2020Quote: ... Bone marrow cells were first incubated for 15 minutes with Human TruStain FcX (BioLegend #422302) and TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Genetics 2021Quote: ... The cell suspension was incubated in Human TruStain FcX blocking solution (1:20, Biolegend; 422302) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... confluent BCR-expressing B cells were FACS sorted on IgM (APC anti-human IgM; BioLegend) and kappa light chain (PE anti-human kappa light chain ...
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bioRxiv - Immunology 2021Quote: ... and 1×106 cells were incubated sequentially or together with Human Trustain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406) ...
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bioRxiv - Immunology 2020Quote: ... with 100ug of antibodies against specific human NK cells expressing NKp46 Domain 1 (Biolegend, 9E2) and/or human CD8α (Bioxcell ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and then incubated with Human TruStain FcX Blocking Solution (Biolegend) for 10 mins ...
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bioRxiv - Genomics 2022Quote: ... Ligand expression was confirmed for engineered 293T cells using anti-human CD28-PE (302907, Biolegend), anti-human CD20-PE (302306 ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with anti-human IgG-APC (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... The TALL-104 cells were first stimulated with anti-human CD3 antibody (5μg/ml) (Biolegend) and anti-human CD28 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with anti-CD1a (FITC anti-human CD1a Antibody, clone: HI149, BioLegend), anti-CD93 (PE conjugated anti-human CD93 Antibody ...
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bioRxiv - Immunology 2023Quote: ... 100 μL of cell suspension was mixed with Pacific Blue anti-human Ki-67 (Biolegend), followed by incubation at room temperature in the dark for 30 min ...
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bioRxiv - Immunology 2023Quote: ... for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes, BioLegend). Surface staining was performed with fluorophore-conjugated antibodies (Table S1 ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used to stain the cells: BV711-conjugated anti-human CD4 (BioLegend); APC-conjugated anti-human PD1 (BioLegend) ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed and permeabilized cells were stained with PE anti-human Ki-67 antibody (BioLegend #350504) diluted 1:10 in the BD Perm/Wash buffer from the kit for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... RAW 264.7 cells were seeded into 6-well plates at a density of 7.5 x 105 cells/well and incubated overnight with 200 U/mL of recombinant murine IFNγ (Biolegend, San Diego, CA) to promote M1-polarization and then infected with opsonized Cn for 2 h at 3:1 MOI or mock infected with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... M- CSF and GM-CSF differentiated BM cells were cultured with complete medium +10ng/ml recombinant mouse M-CSF (Biolegend, cat.no. 576408) or +20ng/ml recombinant GM-CSF (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with oligonucleotide-tagged anti-VCAM1 and anti-CD24 antibodies (BioLegend, Supplementary Table 5) on ice for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... as well as 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend) listed in Supplementary Table 3 were added and cell pellets were suspended ...
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bioRxiv - Immunology 2020Quote: ... OVA and HEL were biotinylated in house to ∼1 biotin per antigen molecule and tetramers constructed as described [14] using streptavidin-PE (Biolegend, CA, USA), streptavidin-APC (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mouse ICAM-1 and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1 was from R&D Systems.