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Citations for BioLegend :
101 - 150 of 2493 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an 18-hour incubation at 4°C with mouse anti-6E10 antibody (1:1000 in 2% NHS, BioLegend #803001). After three PBS washes ...
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bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/ml IL-5 (Biolegend; 581504), and 20 ng/ml IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin cyanine 5 (5 μg/mL, BioLegend) for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Cell Biology 2024Quote: ... were coated overnight at 4 °C with 100 μl Tau-12 capture antibody per well (2 μg/ml; Biolegend, cat. no 806502) in 20 mM NaH2PO4 pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... We then blocked the brains in 5 % goat serum for 2 h and stained with mouse anti-Aβ42 (6E10) (1:500, BioLegend, CA, USA) in PBST on a shaker at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2024Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
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bioRxiv - Immunology 2021Quote: ... CD11a (M17/4, Biolegend), CD27 (LG-7F9) ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 (BioLegend) were prepared in PBS at concentrations of 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD11a (M17/4; Biolegend), and CD69 (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (BioLegend, 574004), IL-13 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... #3 and #4 (Biolegend) were spiked into individual samples ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 and 5 μg/mL Brefeldin A (BFA; BioLegend) was added during the last 6 h of stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μL of interleukin-4 (IL-4, 50 ng/mL, BioLegend, cat. 574002) and M-CSF (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant murine IL-4 (Biolegend) was combined with rat IgG1 mAb to IL-4 ...
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bioRxiv - Microbiology 2021Quote: ... IL-4 APC (Biolegend, #504106), IL-17 PECy7 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... CD4 ([clone OKT-4] BioLegend), CD3 ([clone SP34-2] BD) ...
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bioRxiv - Immunology 2020Quote: ... anti-Vβ5 (BioLegend, MR9-4), and anti-Nur77 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... AF488-IL-4 (11B11, Biolegend), PerCP-eFluor710-IL-13 (eBio13A ...
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bioRxiv - Neuroscience 2022Quote: ... IL-4 (clone 11B11, BioLegend), IL-17a (clone eBiol7B7 ...
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bioRxiv - Immunology 2022Quote: ... CTLA-4 (UC10-4B9, Biolegend); FcR Block (120-000-826 ...
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bioRxiv - Immunology 2023Quote: ... α4-1BB-APC (17B5, Biolegend), αCD107a-APC-Cy7 (1D4B ...
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bioRxiv - Immunology 2024Quote: ... IL-4 (Biolegend cat #: 500818), and IL-17A (Biolegend cat # ...
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bioRxiv - Immunology 2024Quote: ... TIM-4 (RMT4-54, Biolegend) 1:200 ...
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bioRxiv - Immunology 2024Quote: ... IL-4 (clone: 11B11; BioLegend), IL-17A (clone ...
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bioRxiv - Immunology 2024Quote: ... CD137 (BV650, 4-1BB, Biolegend), CD8 (BV605 ...