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Citations for BioLegend :
1401 - 1450 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD3-BV605 (Clone: OKT3; Biolegend, 317322), Verified Reactivity ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD3-PE/Cy7 (1:100, Biolegend, clone HIT3a), anti-CD2-PE/Cy5 (1:160 ...
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bioRxiv - Cancer Biology 2023Quote: ... PE anti-CD3 (1:300, Biolegend, #300441, clone UCHT1), Alexa Fluor® 647 anti-CD45 (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD3-PE/Cy5 (1:320, Biolegend, clone HIT3a), anti-CD4-PE/Cy5 (1:160 ...
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bioRxiv - Immunology 2023Quote: ... APC anti-human CD3 (300412, BioLegend; 1 μL/well), and PE/Dazzle™ 594 anti-human CD8 (301058 ...
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bioRxiv - Cell Biology 2023Quote: Mouse anti-human CD3-BV605 (Clone: OKT3; Biolegend, 317322), Verified Reactivity ...
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bioRxiv - Cell Biology 2024Quote: Rat anti-CD3 (BioLegend, clone 17A2, #100201, 1:100), Rat anti-CD4 (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... CD3-PerCP/Cy5.5 (BioLegend, Catalog No. 100327, 1/160), CD8-PE/Cy7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Slices were processed using FITC-anti-CD3 (Biolegend 344804) and PE-anti-EPCAM (Biolegend 324206 ...
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bioRxiv - Immunology 2020Quote: ... TCRβ chain-PE/Cy7 (clone: H57-597; dilution: 1:100, BioLegend), CD4-Alexa Fluor 488 (clone ...
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bioRxiv - Microbiology 2022Quote: ... APC/Cyanine7 anti-mouse TCR β chain (Clone H57-597, Biolegend), PE/Cyanine7 anti-mouse IFNγ (Clone XMG1.2 ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Immunology 2022Quote: ... for 5 min on ice prior to immunostaining with a surface stain cocktail containing the following antibodies: I-A/I-E Alexa Fluor 488(Biolegend, cat.107616), CD8a PerCP/Cyanine5.5 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... tissues were blocked for endogenous avidin and biotin using an avidin biotin blocking kit according to the manufacturer’s instructions (BioLegend). Sections were then incubated with biotin conjugated goat anti-chicken (Vector Labs ...
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bioRxiv - Immunology 2019Quote: 106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat ...
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bioRxiv - Neuroscience 2023Quote: ... cells were subject to surface staining by being incubated with a cocktail of anti-CD11b (Biolegend, USA, Catalog #101225), anti-CD45 (Biolegend ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Cell Biology 2022Quote: ... Erythroid precursors were isolated using negative selection by lineage-depletion with biotin- conjugated antibodies and MojoSort streptavidin-conjugated magnetic nanobeads (Biolegend): Anti-mouse CD3e (clone 145-2C11) ...
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Immunology 2023Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Immunology 2021Quote: ... P815 cells were used as target cells for human CTLs and pulsed for 1h at 37°C with 1µg/ml anti-CD3 antibody (clone UCHT1, Biolegend, Cat no. 300438). They were washed and resuspended at 1x105 cells/ml in killing assay media (RPMI without phenol red + 2% FCS ...
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bioRxiv - Biophysics 2020Quote: ... The lineage depleted fraction was stained with 13 markers for lineage-specfic monoclonal antibodies FITC (CD2, CD3, CD4, CD11b, CD14, CD15, CD24, CD10, CD41, CD20, CD66c, CD127, CD33) (Biolegend and BD) and Pacific blue conjugated anti-CD34 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 5 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), which is then washed off with PBS ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... CD19-expressing K562 (CAR Activation) or CD64- and CD80-expressing K562 previously loaded with anti-CD3 antibody (OKT3, Biolegend, San Diego, CA) at 1 μg/ml for 1 hour 39 (TCR/CD28 Activation ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Immunology 2019Quote: ... Total cells were counted by hemacytometer and incubated with PE-conjugated-anti-CD3 (Clone 17A2, BioLegend, San Diego, CA) at a concentration of 1 µg / 106 cells for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: 1×106 (for immunoblot) or 1.8×107 (for immunoprecipitation) Jurkat Cells were incubated with anti-CD3 (1 μg/ml, OKT3, 317325, BioLegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with FACS buffer and stained with anti-CD3 PerCp-Cy5.5 and anti-CD279 BV421 (Biolegend) and Live/dead fixable near IR APC-Cy7 in FACS buffer for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4+ T cells were co-cultured with APCs at indicated ratio in the presence of 0.5 μg/mL (anti-CD3 BioLegend), 0.5ng/mL TGFβ (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... The samples were washed with Cell Staining Buffer and stained with anti-mouse TCR β chain-Alexa Fluor® 647 (Biolegend) at a concentration of 0.02μg/μl ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were cultured in the presence of blocking CD25 mAb (Biolegend). After 24h of stimulation ...
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bioRxiv - Immunology 2022Quote: ... enriched naïve WTKL CD4+ T-cells were labeled with Tag-It Violet (BioLegend Inc) while E641:I-Ab+ (null ...
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bioRxiv - Immunology 2020Quote: ... T- and B-cells were depleted in CB MNCs via MojoSortTM Streptavidin Nanobeads (BioLegend) using the supplier’s negative selection protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... the MojoSort™ Human CD4 naïve T cell isolation kit (catalog; 480041) (BioLegend, CA) was used.
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bioRxiv - Immunology 2020Quote: ... T cell panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend), CD3-BUV737 (clone ...
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bioRxiv - Genomics 2023Quote: ... For day 19 post CAR T cell delivery analyses anti-human CD45-FITC (Biolegend) staining was added to the above panels to increase sensitivity of CAR T cell detection ...