Labshake search
Citations for BioLegend :
1251 - 1300 of 9381 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702 ...
-
bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602 ...
-
bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
-
bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
-
bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of CD3 antibody (Cat≠317307; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
-
bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA ...
-
bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
-
bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
-
bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
-
bioRxiv - Immunology 2023Quote: ... Primary antibodies used were: Vimentin 1:500 (Standard BioTools, #3143027D), CD3 1:250 (Dako, #A0452, Lysozyme 1:400 (Biolegend, #860001), CD11c 1:300 (Abcam ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with cold PBS and then stained with a cocktail of fluorescently labeled antibodies including: anti-human CD3-PE-Cy7 (Clone: OKT3, Biolegend), Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone ...
-
bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... CD3/CD28 Beads were magnetically removed and transduction efficacy was analyzed by staining with fluorophore-conjugated antibodies (all from Biolegend) and 7-AAD staining solution (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
-
bioRxiv - Genomics 2024Quote: ... NTC and Rosa26 cell viability was assessed and surface expression of CD3 and B2M was compared to NTC and Rosa26 using fluorescence-labelled antibodies (anti-CD3 BV421, UCTH1 clone; anti-B2M FITC, 2M2 clone; both BioLegend) and flow cytometry (IntelliCyt iQue Screener Plus ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: Alexa Fluor 488 anti-mouse CD3 (17A2) (1:100) (BioLegend, San Diego, CA), PE anti-mouse CD4 (GK1.5 ...
-
bioRxiv - Genomics 2019Quote: ... CD19-positive cells were removed (StemCell) and CD4+ T cells were enriched with magnetic beads (Biolegend) prior to sorting naïve T cells (CD4+CD45RO- ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proliferation of CD8+ T cells was determined using the CFSE Cell Division Tracker Kit (Biolegend 423801) according to the manufacturer’s protocols.
-
bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
-
bioRxiv - Immunology 2022Quote: ... CD19-positive cells were removed (StemCell) and CD4+ T cells were enriched with magnetic beads (Biolegend) prior to sorting Tfh cells (CD4+CD25- CXCR5hiPD1hi) ...
-
bioRxiv - Immunology 2023Quote: ... single cell suspensions were either enriched for γδ T cells using Mojo Sort magnetic beads (BioLegend) for depletion of CD4 and CD8 T cells or sorter for Vγ4 T cells ...
-
bioRxiv - Immunology 2023Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
-
bioRxiv - Immunology 2023Quote: ... Sorted γδ T cells were stained with 2.5 nM CFSE Cell Division Tracker Kit (423801, BioLegend) for 20 min at 37 °C immediately after sorting ...
-
bioRxiv - Immunology 2020Quote: ... and Ig light chain λ-PE (Biolegend, San Diego, USA).
-
Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... and TCR β chain (H57-597) were purchased from BioLegend. Anti-Bcl6 (clone K112-91) ...
-
bioRxiv - Genomics 2020Quote: ... Cells were then washed once with full staining buffer (3% FBS in PBS with 2 mM EDTA) and surface antigens were stained for 30 min on ice using the following antibodies: gdTCR-BV421 (B1, Biolegend, 331218), CD45-Pacific Blue (HI30 ...
-
bioRxiv - Bioengineering 2022Quote: ... Single cell suspensions of pericytes were stained for 30 minutes at RT using fluorescence-labelled antibodies for pericyte surface markers (488-NG2 from Invitrogen, APC PDGFR-β and APC CD105 from Biolegend) and DAPI for cell viability ...
-
bioRxiv - Immunology 2023Quote: ... catalog #13-0870-T500) and surface stained with the following antibodies in an appropriate combination of fluorochromes: CD45.2 (BioLegend, clone 104), Epcam (BioLegend ...
-
bioRxiv - Cell Biology 2024Quote: ... The cell suspensions were then co-incubated with fixable viability dye (eFluor™ 780, 65-0865-14, eBioscience) and antibodies against surface markers CD45(PE/Cy7, 147703, BioLegend), Cd3e(APC ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples to be stained for surface markers were washed in DPBS and resuspended in Cell Staining Buffer (Biolegend). Surface marker stains were prepared in BD /Cytoperm™ solution (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... 3-4 × 106 splenocytes after cell surface staining were fixed with Cyto-Fast Fix/Perm buffer (BioLegend, USA) for 20 min at room temperature and washed twice with FACS staining buffer ...
-
bioRxiv - Immunology 2023Quote: ... Cells were labelled with live/dead and surface stains prior to fixation and permeabilization with fixation buffer (Biolegend) and intracellular staining permeabilization wash buffer (Biolegend) ...
-
bioRxiv - Immunology 2023Quote: Surface expression of mouse or human CD47 on murine tumor cell lines was assessed using MIAP301-PE (Biolegend) and CC2C6-APC (Biolegend ...
-
bioRxiv - Immunology 2024Quote: ... target cells were identified by flow cytometric surface staining with CD38-PE (Fisher) and CD138-PERCP-Cy5.5 (Biolegend) (DL-101 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed and surface stained with CD4-PE-Cy5 (Clone RPA-T4, BioLegend 300510, 1:400), CD69-BUV496 (Clone FN50 ...
-
bioRxiv - Genomics 2020Quote: ... Tissues were immunostained with the primary antibodies rat anti-mouse CD31:Biotin (clone 390, Biolegend, San Diego, CA, USA) and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson ...
-
bioRxiv - Pathology 2022Quote: ... The following antibodies were used at 1:200 unless specified: anti-CD9 (clone MZ3, FITC, APC/Fire750, biotin; BioLegend), anti-CD31 (clone 390 ...
-
bioRxiv - Cell Biology 2022Quote: ... MACS was performed in a two-step process using anti-F4/80-biotin coupled antibody (clone BM8, Biolegend, 123106) and Streptavidin microbeads (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: ... enriched with the MojoSort Mouse CD4 Naïve T Cell Isolation Kit (480040 BioLegend) and were purified by flow-cytometry ...
-
bioRxiv - Cancer Biology 2020Quote: ... Mouse T cells (syngeneic MB49 model of urothelial carcinoma): CD45 (clone PerCP, Biolegend), CD25 (clone PC61 ...
-
bioRxiv - Immunology 2022Quote: ... naïve CD4+ or naive CD8+ T cell enrichment kit (Biolegend, San Diego, CA). Cell purity for all enrichments ranged between 90-98% ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then the CD4+ T cells were stained with anti-CD4-APC (Biolegend, 100412) and anti-CD25-PerCP-CY5.5 (BD ...
-
bioRxiv - Immunology 2020Quote: ... NOD.Ai4 CD8+ T-cells were identified with anti-CD8 (BioLegend, San Diego, CA) and tetramer staining ...
-
bioRxiv - Cell Biology 2022Quote: ... Fixed developing T cells were resuspended with APC TCRβ (1:400, Biolegend, 109212) in 10% permeabilization buffer at room temperature for 1 hr ...
-
bioRxiv - Immunology 2021Quote: ... or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, San Diego, CA). Purity of the isolate CD8+ cells constantly exceeded 95%.
-
bioRxiv - Immunology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit and SDF-1α were from BioLegend. Recombinant human VCAM-1-Fc ...
-
bioRxiv - Immunology 2022Quote: ... T cells were activated with plate-bound anti-CD3ε (clone 145-2C11; Biolegend) and 0.5 µg/ml of soluble anti-CD28 (clone 37.51 ...