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Citations for BioLegend :
1401 - 1450 of 1985 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Neuroscience 2020Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Immunology 2021Quote: ... enriched with the MojoSort Mouse CD4 Naïve T Cell Isolation Kit (480040 BioLegend) and were purified by flow-cytometry ...
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bioRxiv - Immunology 2021Quote: ... washed then stained with a viability dye (Zombie Aqua Fixable Viability Kit, Biolegend) followed by surface staining ...
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bioRxiv - Immunology 2019Quote: ... Annexin V staining was performed using an APC Annexin V staining kit (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were first enriched using Mouse CD4 T Cell Isolation Kit (Biolegend) before electronic sorting ...
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bioRxiv - Immunology 2021Quote: ... Cell viability was assessed using the Zombie NIR™ Fixable Viability Kit (Biolegend) as per the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... Primary CD14+ monocytes were isolated using Biolegend MojoSort magnetic isolation kit (Biolegend, 480094) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Live/dead (LD) cells were identified with Zombie aqua fixable viability kit (Biolegend). CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested and stained with Live/Dead Aqua staining kit from BioLegend as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... and TNF-α were measured using the corresponding human ELISA kits (Biolegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: The cells were stained with Zombie Green™ Fixable Viability Kit (BioLegend, USA) in PBS for 15 min at RT and in the dark using a dilution of 1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... conditioned medium was collected and analyzed using mouse TNF-α ELISA kit (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Cell Biology 2021Quote: ... they were stained with Zombie Violet™ Fixable Viability Kit (1:1000, BioLegend) for 30 minutes on ice during secondary antibody staining ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ or naive CD8+ T cell enrichment kit (Biolegend, San Diego, CA). Cell purity for all enrichments ranged between 90-98% ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated with Zombie Aqua™ Fixable Viability Kit (Biolegend, #423101, 1:1000) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with fluorescent antibodies: APC-conjugated anti-mouse CD117 (c-Kit) (Biolegend) and PE-Cy7-conjugated anti-mouse Ly-6A/E (Sca-1 ...
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bioRxiv - Immunology 2019Quote: ... Type III IFN proteins were measured using a custom-designed LEGENDplex kit (BioLegend). IFN-β was measured using the Verikine ELISA (PBL) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed with the True Nuclear Transcription Factor staining kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... TILs and N-TILs were stained using Zombie Aqua fixable viability kit (Biolegend), following which surface staining was performed with a mixture of fluorescence-conjugated antibodies (BD Biosciences or BioLegend) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were surface stained with the Zombie Aqua Fixable Viability Kit (Biolegend, #423101) for 0.5hr at RT,1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: The LEGENDplexTM Human Inflammation (#740809) and Growth Factor (#740180) kits (BioLegend, CA, USA) were used to detect cytokines and growth factors in conditioned media from 3D MDMi and ReNcell VM mono- and co-cultures ...
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bioRxiv - Immunology 2021Quote: ... were stained for live/dead dye (Zombie Aqua fixable viability kit, #423102 BioLegend) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The green dye CFSE Cell Division Tracker Kit (BioLegend, San Diego, California, USA) and DAPI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Moncytes were isolated by MojoSort CD14 positive selection kits (Biolegend, California, United States) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, San Diego, CA). Purity of the isolate CD8+ cells constantly exceeded 95%.
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bioRxiv - Immunology 2021Quote: The following antibodies and kits were purchased from Biolegend (San Diego, CA, USA): APC/Cy7 anti-CD4 (clone GK1.5) ...
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bioRxiv - Immunology 2021Quote: ... the thymocytes were fixed and permeabilized using a Fixation/Permeabilization Solution Kit (Biolegend), followed by staining using indicated antibodies ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and stained with Zombie NIR™ Fixable Viability Kit (BioLegend) to label dead cells ...
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bioRxiv - Immunology 2020Quote: ... Dead cells were excluded using Zombie Aqua Fixable Viability Kit (BioLegend, Cat: 423102). For intracellular cytokine stainings ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were first excluded using Zombie Aqua™ Fixable Viability Kit (BioLegend). Cells were then incubated with fluorescence conjugated antibodies in HBSS containing 2% BCS at 4℃ for 30 minutes to stain surface markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with Zombie NIR™ Fixable Viability Kit (Biolegend, CA). Prior to acquisition ...
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bioRxiv - Cell Biology 2021Quote: ... Granzyme B and perforin were determined by ELISA kits (ABclonal, Biolegend, and Abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit and SDF-1α were from BioLegend. Recombinant human VCAM-1-Fc ...
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bioRxiv - Immunology 2022Quote: ... IL-6 and TNF murine ELISA kits were purchased from Biolegend (#431304, #430904). Assays were performed in duplicate when possible ...