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Citations for BioLegend :
1251 - 1300 of 1985 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... ELISA kits for detection of mouse IL-1β was from BioLegend and for mouse IL-12p70 and human IL-1β were from BD ...
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bioRxiv - Immunology 2020Quote: ... Dead cells were stained with Zombie NIR Fixable Viability kit (Biolegend) (1:400 in PBS ...
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bioRxiv - Genomics 2021Quote: ... in combination with the Zombie Violet Fixable Viability Kit (BioLegend 423113). The Human MSC Analysis Kit assesses the surface markers CD90 ...
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bioRxiv - Immunology 2022Quote: ... and LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: Cells were stained with Zombie Aqua™ Fixable Viability Kit (Biolegend), to exclude apoptotic and death cells ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s protocol (Zombie NIR Fixable Viability Kit, BioLegend). After 30 min at 4ºC in the dark ...
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bioRxiv - Molecular Biology 2022Quote: Apoptosis was determined using the Annexin V-APC PI kit (Biolegend). 48 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained using Zombie Aqua Live/Dead kit (Biolegend #423101) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... permeabilized and stained using True-Nuclear Transcription Factor buffer kit (Biolegend) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Apoptosis analysis was performed by using Annexin-V-APC kit (BioLegend) as described in manufacturers protocol for all treatment conditions and cell lines ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with Zombie Aqua Fixable Viability Kit (BioLegend, 423114) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with a Zombie Red viability dye kit (Biolegend), followed by fixation with fixation solution (eBioscience-Thermo) ...
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bioRxiv - Cancer Biology 2023Quote: ... FITC Annexin V PI Apoptosis Detection Kit (BioLegend, Cat. No. 640914) was used to study the apoptosis induction caused by BMN-673 following manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... For the process of permeabilization either FoxP3 Fix/Perm kit (Biolegend) or 2% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis staining was performed using 7AAD-annexinV-APC kit (BioLegend®). Data analysis was conducted using FlowJo™ (TreeStar) ...
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bioRxiv - Immunology 2022Quote: ... after which cytokine levels were measured with LEGENDplex kits from Biolegend. Specifically ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with Zombie AquaTM Fixable Viability Kit (BioLegend), followed by extracellular staining ...
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bioRxiv - Immunology 2023Quote: ... using a live/dead dye (Zombie NIR Fixable Viability Kit, BioLegend) to enable exclusion of dead cells from downstream analysis ...
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bioRxiv - Immunology 2023Quote: ... All cytokine ELISA kits were purchased from BioLegend (San Diego, CA), except for IL-13 (eBioscience).
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bioRxiv - Immunology 2024Quote: ... ELISA kits and all fluorescently tagged antibodies were purchased from BioLegend. Pam3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Secreted proteins were measured using the ELISA MAX Deluxe kits (Biolegend). Absorbance was measured at 450 nm and 570 nm using a Synergy H1 plate reader (BioTek) ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with Zombie NIR™ Fixable Viability Kit (Biolegend, Cat. 423105) to assess live vs ...
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend), anti-CD3ε antibody (Ab ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated for 16 hours at 37°C in the presence of 2 uM monensin (Biolegend; San Diego, CA), 5 μg/mL Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... T cells were pelleted in a V-bottom plate and resuspended in 40 µl PBS with 2% BSA and fluorescent 9V pMHC tetramers that were produced with Streptavidin-PE (Biolegend, 405204) and used at a predetermined dilution (1:100-1:1000) ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2019Quote: ... They were blocked for 1 hour at room temperature in 2% donkey serum and CD16/CD32 (Biolegend, Clone 93, 1:200). Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF771 goat anti-IL-1R ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...