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Citations for BioLegend :
1401 - 1450 of 8635 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Purity of isolated T cell fractions was verified by staining with fluorophore-conjugated antibodies (all from Biolegend, San Diego, CA, USA) and 7-AAD staining solution (130-111-568 ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Immunology 2023Quote: ... All labelling was carried out at room temperature (RT) for 10mins protected from light with antibodies diluted in cell staining buffer (Biolegend; 420201). For AnnexinV staining ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 6 x 106 cells were stained with the appropriate cocktail of cell-surface conjugated antibodies in 200µL FWB with the fixable viability dye Zombie Red (BioLegend; 1:1000) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Biophysics 2023Quote: ... then cells were stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, clone 6H2.B4). Stained cells were analyzed using an Attune NxT flow cytometer ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed and stained with an Allophycocyanin (APC) labeled secondary antibody (Donkey anti-rabbit IgG DyLight™ 649, Biolegend 406406) for 30-45 minutes at room temperature in 2% normal donkey serum ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with standard antibodies for immune and vascular lineage exclusion: anti-CD45-PE/Cy7 (BioLegend, 304016, 1:200), anti-CD31-PE/Cy7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
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bioRxiv - Immunology 2024Quote: ... the enriched epithelial cell suspension was stained for flow cytometry cell sorting with the following antibodies and dyes: anti-EpCAM-APC-Cy7 clone G8.8 (BioLegend, cat. 118218), anti-CD45-APC clone 30-F11 (BD Biosciences ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with fluorophore-conjugated antibodies (CD81 antibody BioLegend 349501 or Cd44 antibody Biolegend 103023) for 1 hour followed by a PBS wash ...
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bioRxiv - Neuroscience 2024Quote: ... PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody (103222) and APC anti-mouse CD3 Antibody (100236) antibodies (Biolegend, San Diego, CA) were utilized for the lymphoid populations ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were as follow: TUJ1 (Tubb3/βIII tubulin) antibody (BioLegend catalog #802001, RRID:AB_291637), FABP7 (Thermo Fisher Scientific Cat #PA5-24949 ...
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bioRxiv - Microbiology 2019Quote: ... rat monoclonal antibody against CD45 antibody (Clone 30-F11) was purchased from Biolegend. FITC-conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were: Rabbit monoclonal antibody specific for Aβ40 (1:2,000, BioLegend 867802) and rabbit monoclonal antibody specific for Aβ42 (1:2,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibody staining was performed with Alexa Fluor® 647-streptavidin antibody (BioLegend). Dead cells were stained with eFluor 450-fixable viability dye (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-pan-cytokeratin-AF532 antibody (Nanostring) and anti-CD45-AF594 antibody (BioLegend, 103144) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibody was prepared by conjugating anti-rat IgG2 antibody (BioLegend, 407502) with DyLight 633 (Thermofisher ...
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bioRxiv - Bioengineering 2024Quote: ... FITC anti-mouse CD8a antibody and APC/Cyanine7 anti-mouse CD4 antibody (BioLegend) in PBS ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
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bioRxiv - Immunology 2020Quote: ... CAFs were co-cultured overnight with CD4+ T cells at a 1:1 ratio in the presence of pan-HLA antibody (5μg/ml, Biolegend, Cat. No.361702) or GC1q R antibody (10μg/ml ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...
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bioRxiv - Genomics 2020Quote: ... Expression of cell surface markers in resting and CD3/CD28 activated cells was made by flow cytometry using fluorescent-coupled antibodies against CD4 (Biolegend, Cat: 100434), CD3 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... NK were co-cultured for 8 h with target cells in the presence of CD107a PerCP-Cy5.5 antibody (H4A3, BioLegend, 250 ng/ml) and 2.5 ng/ml IL-15 (R&D) ...
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bioRxiv - Systems Biology 2020Quote: ... up to 1×106 EnSC/100 µL of Magnetic Bead Buffer (0.5% BSA in PBS) were combined with 5 µL/1×106 cells phycoerythrin (PE) conjugated anti-human SUSD2 antibody (BioLegend, London, UK). After 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained at room temperature for 45 minutes with the following antibodies: CD3 BV650 (OKT3; Biolegend, San Diego, CA, USA), CD4 BV785 (OKT4 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained at room temperature for 45 min with the following antibodies: CD3 BV650 (OKT3; BioLegend, San Diego, CA, USA), CD4 BV785 (OKT4 ...
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently resuspended in 100 µl of FACS buffer with a secondary anti-mouse-APC antibody (BioLegend, catalogue number-405308). The cells were incubated at 4 °C for 30 minutes on ice and washed with PBS as before ...
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bioRxiv - Immunology 2020Quote: ... We divided the unstimulated cells per donor in half and stained one tube of unstimulated cells and one tube of stimulated cells per donor with an intracellular antibody master mix consisting of anti-IL17A – APC (BioLegend, Clone BL168) and PE conjugated to either anti-IL-4 ...
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bioRxiv - Immunology 2020Quote: ... CD19+CD138+ plasma cells were isolated from PBMCs by positive selection using biotin conjugated CD138 antibody (352322, Biolegend, San Diego, CA, USA) and Easysep release human biotin positive selection cocktail (17653 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Cell Biology 2020Quote: ... a 6-colour flow cytometry panel was performed on a Bio-Rad ZE5 Cell Analyzer using antibodies for CD56 (Clone HCD56, Brilliant Violet 605, BioLegend, 1:200), CD3 (Clone SK7 ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... some samples were additionally stained with antibodies to enrich for T cell populations (NB124, NB125, NB138 with CD3-PE (Biolegend, cat#300308); NB124 with TCRγδ-BV421 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 20– 30 min in the dark on ice with the conjugated antibodies as follows (BioLegend unless denoted otherwise) following manufacturer’s recommended concentrations ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4degC with the following fluorochrome conjugated primary anti-mouse antibodies for 45 minutes (all Biolegend, San Diego, CA): anti-CD45 PE-CF594 (clone 30-F11) ...
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bioRxiv - Bioengineering 2023Quote: ... the CNP labeled cells were spin washed and incubated with 10μgꞏml−1 of AF488 conjugated anti-CD3 antibodies (Biolegend, Cat # 300415, clone UCHT1) in 2% BSA/PBS on ice for 30min and then spin washed ...