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Citations for BioLegend :
1251 - 1300 of 8635 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2019Quote: ... labelled in-house with Cy5 and the following antibodies were used to stain the cells: CD45::AF700 (30-F11, BioLegend), EpCAM::PerCPCy5.5 (G8.8 ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... To prevent antibodies from binding to Fc-receptors we treated the cell suspension with Fc-block (Biolegend #101301; 1:50) prior using other antibodies ...
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bioRxiv - Immunology 2019Quote: ... Approximately 106 cells/sample were re-suspended in PBS and dead cells removed using amine-reactive dye NIR Zombie (1:500 dilution in the antibody master mix; #423105, BioLegend). Live cells were stained with the following cell surface marker specific antibodies ...
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bioRxiv - Immunology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL antibody (141606, BioLegend) and PerCP-Cy5.5-conjugated anti-mouse CD86 antibody (105027 ...
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bioRxiv - Immunology 2021Quote: ... transfected cells were stained with aqua dye for live/dead discrimination and corresponding antibodies-MICA/MICB (Clone 6D4, PE, BioLegend) or ULBP-1 (clone 170818 ...
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bioRxiv - Neuroscience 2020Quote: ... Differentiated cortical cells were validated for neuronal and cortical markers by immunostaining using antibodies for TUBB3 (BioLegend, Cat#MMS-435P), TBR1 (Abcam ...
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bioRxiv - Bioengineering 2020Quote: ... cells were recovered from wells and were incubated at 4°C for 30 minutes with antiCD3 antibody (Biolegend, CA, USA) and then were examined by flow cytometry.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were assayed for PD-L1 surface expression via flow cytometry using PDL1-BV605 antibody (Biolegend Inc, catalog number:124321) using IgG beads (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Single cell suspension was generated from adult lungs and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Molecular Biology 2021Quote: ... CD4+ or CD8+ cells were stained with an APC-Cy7-labeled anti-CD4 and FITC-labeled anti-CD8 antibody (Biolegend), respectively ...
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bioRxiv - Genomics 2021Quote: ... Then cells were stained with the following cell surface marker antibodies for 30 min on ice (1:100 dilution, all from BioLegend): CD11b-PE (301306 ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Samples sorted for 10x Genomics single-cell RNAseq were individually labelled with an oligonucleotide-linked hashing antibody (Totalseq-C, Biolegend) in addition to the staining cocktail and sorted into a single tube according to the gating strategy shown in Fig ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716], anti-CD3e FITC [Biolegend, 100306], anti-CD4 BV786 [Biolegend, 100552] ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: Cells were stained with the following surface marker antibodies: anti-mouse CD45 (BV510™, BioLegend, clone 30-F11, cat# 103138), anti-mouse CD11c (BV785™ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Immunology 2023Quote: ... Murine cells were stained for 30 min at 4°C with the following antibodies (all from Biolegend, unless otherwise stated): CD3 APC(Clone:17A2 Cat:100236 ...
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bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
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bioRxiv - Immunology 2023Quote: ... The phenotype of differentiated HL-60 cells was assessed by flow cytometry using mouse antihuman CD35 PE conjugated antibody (BioLegend) and mouse anti-human CD71 APC conjugated antibody (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: Differentiated cortical cells were validated for neuronal and cortical markers by immunostaining using antibodies for TUBB3 (BioLegend, Cat#MMS-435P), TBR1 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... 5000 cells of each compartment were labeled with anti-mouse hashtag oligonucleotide (HTO) antibodies (TotalSeq™-C0301/C0302/C0303, Biolegend) and pooled together ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following fluorochrome-conjugated antibodies: APC/Fire™ 750 anti-human CD324 (E-Cadherin) (Biolegend 324122), PE anti-human CD326 (EpCAM ...
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bioRxiv - Immunology 2023Quote: ... HLA-expressing cells were enriched with a positive magnetic sort using the biotinylated pan-HLA antibody (clone W6/32, Biolegend) in conjunction with the ThermoFisher CELLection Dynabead cell isolation kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Immunology 2023Quote: Harvested lungs and spleen immune cells were incubated with recommended dilutions of antibodies conjugated to fluorophores: CD3-BV510 (BioLegend, #100234), CD4-BV785 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8β cells were depleted from the T-IEL compartment using CD4 and CD8β biotinylated antibodies and magnetic streptavidin nanobeads (MojoSort) (Biolegend). CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... EMT phenotype of in vivo isolated tumor cells was analyzed using the same antibody panel implemented with anti-mouse CD45 (30-F11; Biolegend), CD90.1 (OX-7 ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Cell Biology 2023Quote: ... Long bones and iliac crests were isolated and bone marrow was obtained by centrifugation at 2500g for 40s as previously described.41 Hematopoietic stem and progenitor cells (HSPCs) were separated by lineage depletion using an antibody mixture (Lineage depletion panel, 133307, Biolegend) and magnetic beads (CD4 untouched ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and cells without either EpCam or CD45 markers were sorted using antibodies against EpCam (clone G8.8, Biolegend, San Diego, CA) or CD45 (clone 30-F11 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected at d5 of the culture for the analysis of T cell cytokine production using a combination of commercially available antibodies (Biolegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with a combination of fluorochrome-conjugated antibodies to the following surface markers (from Biolegend unless otherwise indicated): CD3ε (17A2 ...
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bioRxiv - Cancer Biology 2023Quote: ... non-natural killer cells were depleted by incubating the patient’s whole PBMCs sample with the biotin antibody cocktail (BioLegend 480053) followed by incubation with magnetic Streptavidin Nanobeads ...