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Citations for BioLegend :
1351 - 1400 of 3266 citations for IL 12B Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Human Cytokine Panel 2 (BioLegend, San Diego, USA) for cytokine determination and CyQUANT™ LDH assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... rat α-mouse/human PNAd (MECA-79, Biolegend). For detection of horse radish peroxidase or alkaline phosphatase ...
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bioRxiv - Immunology 2023Quote: ... anti-human Integrin β7-APC (clone FIB504, BioLegend), rabbit anti-human TG2 (Pacific Immunology) ...
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bioRxiv - Immunology 2023Quote: ... anti-human CD27-APC/Cy7 (clone O323, BioLegend), anti-human CD38-BUV563 (clone 2B7 ...
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bioRxiv - Neuroscience 2024Quote: ... and Brilliant Violet anti-human CD45 antibody (Biolegend) for gating and live / dead staining ...
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bioRxiv - Biophysics 2024Quote: ... and PE anti-human CD9 (BioLegend, cat. 312106).
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204), or Biotin anti-human CD117 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204) were used ...
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bioRxiv - Immunology 2024Quote: ... and phycoerythrin-conjugated anti-human CD25 (Biolegend, 356103).
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bioRxiv - Immunology 2024Quote: ... plus 20 ng/mL recombinant human BAFF (Biolegend) for 3 days ...
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bioRxiv - Immunology 2024Quote: ... CCL4 (LegendPlex Human proinflammatory chemokines panel, Biolegend #740985). Immunoassays plates were read on a Novocyte (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IL-17α content were determined by multiplex cytokine assays (Biolegend LEGEND plex). T cells were stimulated with anti-CD3/CD28 for 4-6 hours in the presence of brefeldin A (10μM ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected at 2 hours and 4 hours and analyzed using IL-6 (Biolegend). After removal of supernatant ...
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bioRxiv - Immunology 2019Quote: ... and intracellular antibodies included IL-17A-PE (TC11-18H10.1) and IFNγ-APC (XMG1.2) from Biolegend. For innate lymphoid cell analysis ...
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bioRxiv - Genomics 2020Quote: ... and IL-10 in culture supernatants were measured using ELISA Max Deluxe were from Biolegend® (CA ...
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bioRxiv - Immunology 2020Quote: ... they were fixed and permeabilized and stained with V450-A-anti-IL-1β (Biolegend, US). The frequencies monocyte subsets were assessed on a FACS Verse instrument (BD ...
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bioRxiv - Neuroscience 2022Quote: Enzyme-linked immunosorbent assays were performed using the mouse IL-6 ELISA-MAX kit (Biolegend), using manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: NSG mice were administrated intraperitoneally with IL-6 (50 μg /mouse) neutralizing Ab (BioLegend 504512) for two days ...
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bioRxiv - Immunology 2022Quote: Mouse dorsal skin cells were stimulated in culture with 40 ng/mL IL-23 (Biolegend) alone ...
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bioRxiv - Cancer Biology 2021Quote: ... before staining with diverse fluorescent dye-conjugated monoclonal antibodies: anti-IL-6R/APC (#352806 Biolegend), anti-CD38/BV421 (#303526 Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 Brilliant Violet 421 and anti-IFN-γ PE (Biolegend. San Diego, CA).
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bioRxiv - Immunology 2021Quote: IEL and LPL were cultured overnight in complete medium with IL-7 (20ng/ml, Biolegend) + IL-23 (20ng/ml ...
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bioRxiv - Immunology 2021Quote: Murine BMDMs were pretreated with PBS or murine IL-27 (50 ng/ml from Biolegend) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... Th1 differentiation was achieved using 1 μg/mL anti-IL-4 antibody (clone: 11B11, Biolegend), 5 ng/mL IL-2 (PeproTech) ...
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bioRxiv - Immunology 2022Quote: ... daily intratracheal injection with 400 µg/body of anti-IL-10R antibody (clone 1.3B1a, BioLegend) or isotype control (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and then stained with PE-conjugated anti-mouse IL-6 mAb (clone MP5-20F3, Biolegend). Stained cells were analysed using a BD LSR Fortessa cell analyser (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Immunology 2023Quote: Expanded γδ T cell populations were stimulated with 50 ng/mL IL-27 (577402, BioLegend) for 30 minutes at 37 °C ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ and IL-4 production were determined using ELISA kits (BioLegend, San Diego, CA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
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bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human PVR/CD155 (Clone SKII.4, Biolegend), PE anti-human MICA (Clone 159227 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Cell Biology 2020Quote: ... FITC anti-human ICAM-1/CD54 (Clone DX2, Biolegend), FITC anti-human Fas/CD95 (Clone DX2 ...
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bioRxiv - Immunology 2021Quote: ... BV421-conjugated mouse anti-human CD34 (Biolegend, CA, USA), PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Pacific Blue™ anti-human IgD (BioLegend, 348224). After 20 min ...