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Citations for BioLegend :
1601 - 1650 of 3266 citations for IL 12B Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... or PE anti-human CD25 (clone: BC96; BioLegend®) antibodies ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 421 anti-human CD4 Antibody (#357424, BioLegend), PE anti-human TCR Vα24-Jα18 (iNKT cell ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/mL recombinant human thrombopoietin (hTPO) (BioLegend #597402), DNase (100 μg/mL final concentration) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
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bioRxiv - Cell Biology 2020Quote: ... Levels of IL-1β in the media were measured using a commercial ELISA kit from Biolegend.
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bioRxiv - Microbiology 2021Quote: ... rat anti-mouse IL-17-PB (clone TC11-18H10.1, 1:200, cat#506918, BioLegend California, USA), rat anti-mouse TNF-PErCPCy5.5 (clone MP6-XT22 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine antibodies against the flowing markers were used: Alexa Fluor 647-IL-1β (Biolegend, JK1B-1), PE-IL-6 (BD ...
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bioRxiv - Immunology 2022Quote: ... with or without the inclusion of interleukin-4 at 20ng/mL (wt/vol, IL-4, BioLegend), interferon-g at 2.5ng/mL (wt/vol ...
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bioRxiv - Neuroscience 2022Quote: ... + 1% Glutamax + 1% Pen-strep + 0.1 ug/ml IL-34 (PeproTech, #200-34; Biolegend Inc, #577904) + 10 ng/ml GM-CSF (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with biotinylated detection antibodies against IL-12 (Biolegend Cat#505302, clone C17.8) and IL-1β (Biolegend Cat#515801 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Cancer Biology 2023Quote: ... All three groups were co-treated with IL-6 (10 ng/mL, BioLegend cat no. 575706). We included one control group that was treated with DMSO ...
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bioRxiv - Immunology 2023Quote: ... mIL-1β was determined by ELISA MAX™ Standard Set Mouse IL-1β kit (BioLegend# 432601). Human IL-1β and IL-18 were determined with Cisbio HTRF Cisbio Human IL1β (#62HIL1BPET ...
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bioRxiv - Immunology 2023Quote: ... for 3 days at 37°C in the presence of 10 ng/ mL IL-12 (Biolegend).
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bioRxiv - Immunology 2023Quote: ... in the presence of Th17 polarizing cocktails containing recombinant mouse IL-6 (10 ng/ml, Biolegend), mouse IL-1β (10 ng/ml ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β were carried out using ELISA MAXTM Standard Set (Biolegend, San Diego, CA, USA). In brief ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-human CD247 (CD3ζ) mAb (clone 6B10.2) and mouse anti-human EGFR antibody (clone AY13) were purchased from Biolegend (San Diego, CA, USA). Anti-human CD32 mAb (clone 8.26 ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Cancer Biology 2020Quote: ... LPS stimulation was performed in the presence of blocking anti-IL-6 antibody or control IgG (Biolegend), at a concentration of 1ug/mL.
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bioRxiv - Systems Biology 2021Quote: ... Anti-FOXP3 FITC (eBiosciences, UK), anti-IL-10 PE (Miltenyi, UK) and anti-IDO1 AlexaFluor647 (Biolegend, UK) antibodies were used for intranuclear and intracellular staining.
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Cancer Biology 2021Quote: ... and supplemented with murine SCF (10 ng/ml) and IL-3 (10 ng/ml) (Peprotech or BioLegend). The cells were serially passaged for ∼1 month ...
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bioRxiv - Immunology 2021Quote: ... For intracellular cytokine staining we used IL-10 Pe-Cy7 (clone JES3-9D7) (Biolegend, San Diego, CA) and IL-21 alexa fluor 647 (clone 3A3-N2.1 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Microbiology 2022Quote: ... 96-well plates were coated with primary capture antibody against IL-12 (Biolegend Cat#511802, clone C18.2) and IL-1β (Biolegend Cat#503502 ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.
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bioRxiv - Physiology 2023Quote: ... Supernatants were collected and assessed for TNF-α and IL-6 using deluxe ELISA kits from Biolegend. 10mg of perfused liver tissue was rinsed with cold PBS ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibodies against TNF-α (clone: Mab11) and IL-1β (clone: CRM56) from Biolegend for 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... The intracellular staining was performed using anti-IL-17A-APC (Clone TC11-18H10.1) (BioLegend, SanDiego, CA, USA) (1:100) ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Cancer Biology 2021Quote: ... The PE anti-human CD274/PD-L1 (Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b ...
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bioRxiv - Cancer Biology 2021Quote: ... APC anti-human CD3 antibody (clone HIT3a, catalog #300319, BioLegend). The 10x library was sequenced on an Illumina HiSeq 2500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... APC-Fire 750 anti-human CD20 (302357, clone 2H7, BioLegend), and APC-H7 anti-human CD3 (560275 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked with Human TruStain FcX™ (Biolegend), and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone ...
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bioRxiv - Immunology 2021Quote: ... and APC-conjugated mouse anti-human IgD (Biolegend, CA, USA). To determine the purity of CB-SC ...
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bioRxiv - Immunology 2021Quote: ... and Fc blocked for 10 minutes (Human TruStain FcX, Biolegend), and incubated with a cocktail containing CD3-PE (SP34 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD309 (VEGFR2) (Biolegend, clone 7D4-6; 1:50); anti-human CD56 (NCAM ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...