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Citations for BioLegend :
1301 - 1350 of 2285 citations for 17 Hydroxyprogesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend, #317304) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, dilution 1/100), Sca-1 (APC-Cy7 ...
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bioRxiv - Developmental Biology 2020Quote: ... centrifuged in a table top centrifuge at 1,500rpm for 5 minutes and resuspended in 200µl staining solution containing conjugated antibodies Ep-CAM-APC (#118213 Biolegend) and Brilliant violet 785-CD45 (#103149 Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... Red blood cells were lysed on ice for 5 min by using 1x RBC Lysis Buffer (BioLegend, 420301). Bone marrow cells (3×106 per well ...
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bioRxiv - Immunology 2021Quote: ... The PBMC samples were stained with 5 µl of anti-mouse CD3e-PerCP/cy5.5 (Biolegend, USA, cat#100327), NK1.1-PE (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... then ILC2s were diluted 1:5 with PBS containing 1 μg/ml DAPI and Precision Count beads (BioLegend) were added to each sample ...
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bioRxiv - Immunology 2022Quote: ... 5×105-1×106 cells were stained with antibodies to CD45 (D058-1283, Biolegend, San Diego, CSA, USA), CD3 (clone SP34 ...
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bioRxiv - Immunology 2022Quote: ... 5 min prior to euthanasia each mouse was injected retro-orbitally with 5ug of anti-CD45.2-APC (Biolegend) diluted in 200 ul of PBS.
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bioRxiv - Developmental Biology 2019Quote: ... then pre-treated for 5 min on ice with TruStain FcX (anti-mouse CD16/32) antibody (Biolegend, #101319), 1:50 to block Fcγ receptors ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...
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bioRxiv - Immunology 2021Quote: ... Murine monoclonal FITC anti-human CD11b (activated) antibody (IgG1, clone CBRM1/5) was from Biolegend (San Diego, CA). Goat anti-mouse (GαM ...
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bioRxiv - Microbiology 2021Quote: ... The samples were permeabilized for 5 min using Intracellular Staining Perm wash buffer (BioLegend, San Diego, CA, USA). After blocking with 1% BSA in PBST for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... incubated for 1 hour with mouse HRP α-His Tag antibody (1:1000 in 5% milk) (BioLegend, USA) and developed ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2020Quote: ... 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of IL-10-BV421 antibody (Biolegend, 501421) was added for an additional 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL αCD40 (BioLegend), 5 ng/ml TGFβ (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... CA) and phycoerythrin/Cy7 (PECy7)-conjugated anti-mouse CD71 (clone RI7217; 5 μg/mL; BioLegend, San Diego, CA) antibodies were added ...
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bioRxiv - Immunology 2022Quote: ... were injected into the microfluidic circulation in HBSS with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were washed with 1xPBS and blocked with 5% mouse Fc blocking reagent (Biolegend, San Diego, CA) in FACS buffer (1% FBS and 1 mM EDTA in PBS ...
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bioRxiv - Immunology 2024Quote: For immunophenotyping of HD’s and PLWH’s neutrophils the following antibodies were used: PerCPCy5-5-CD15 (HI98, Biolegend #301922), PE-LOX-1 (15C4 ...
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bioRxiv - Biochemistry 2023Quote: ... Fc-receptors were first neutralized by incubating cells on ice for 5-10 minutes with TruStain FcX (BioLegend) at 2 μg/mL in 100 μL of blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... C57Bl/6J mice were injected intravenously with 5 µg of AF488 conjugated to anti-CD4 antibody (100529; Biolegend) and 5 µg AlexaFluor647 conjugated to anti-CD8 antibody (100724 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell suspensions were centrifuged at 1500x rpm for 5 minutes and resuspended in RBC lysis buffer (Biolegend) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were pre-stained with Fc blocker for 10 min in ice followed by staining at 1:300 with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-Pdgfra (25-1401-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Counterstains in PBS were performed directly after secondary antibody incubation (DAPI 1µg/mL 5 minutes, Phalloidin (Biolegend#424203) 5 units/mL) ...
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bioRxiv - Genomics 2024Quote: ... Cells were collected by centrifugation at 300 g for 5 minutes and washed with cell staining buffer (Biolegend). Cells were incubated with FcX (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Macrophages were instead stained with 5 ul of either APC/Cy7-anti human CD16 antibody clone 3G8 (Biolegend), APC-anti human CD206 antibody (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... 5 μl PE-labeled mouse anti-human CD11b fluorescent antibody (cat. no. 301306; 1:1,000 dilution; BioLegend, Inc.) or FITC-labeled mouse anti human CD14 fluorescent antibody (cat ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Physiology 2019Quote: ... overnight at a 1:1 ratio with the indicated target melanoma cells or activated with 1μg/ml plate-bound anti - CD3 (Biolegend, clone: 145-2C11) as indicated in each experiment ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mononuclear cells were collected and washed with complete medium and 104 cells per well in 96 wells plate were plated in the presence of 40ng/ml M-CSF(Biolegend; cat#576402) and 60ng/ml RANKL (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cells in the plasma layer were collected to isolate CD14+ monocytes using a commercial kit (MojoSort human CD14+ monocytes isolation kit, 480047, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Total CD3+ T cells or naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD3 T Cell Isolation Kit or MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend], Zombie NIR fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: Total or naive CD4+ T cells were negatively isolated from spleen using Mojosort Mouse CD4 T Cell Isolation Kit or Mojosort Mouse CD4 Naive T Cell Isolation Kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... all furnished in the same kit (Biolegend #640932). Apoptotic (annexin V-positive ...
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bioRxiv - Immunology 2022Quote: ... or Zombie NIR Fixable Viability Kit (Biolegend, #423105).
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bioRxiv - Immunology 2019Quote: ... using a kit from BioLegend (San Diego, CA).
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bioRxiv - Immunology 2019Quote: ... or Zombie NIR™ Fixable Viability Kit (Biolegend) in PBS followed by washes and surface marker staining in Brilliant™ Stain Buffer (BD) ...
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bioRxiv - Immunology 2019Quote: ... Aqua or red Dead Cell Stain Kits (Biolegend) were used.
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bioRxiv - Bioengineering 2021Quote: A CFSE separation and tracking kit (Biolegend, USA) was used to label PBMCs as a CFSE working solution (5 μM) ...
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bioRxiv - Immunology 2020Quote: ... The Foxp3 Transcription Factor Staining Buffer Kit (Biolegend) was used for staining GATA3 ...