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Citations for BioLegend :
1101 - 1150 of 2285 citations for 17 Hydroxyprogesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... and then stained with anti-FOXP3-BV421 antibody (5 µl/106 cells, clone 206D, BioLegend) using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... NK cells were pre-incubated with CD56 Alexa Fluor 488 (HCD56, Biolegend, 5 μg/ml). Following incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-lineage cocktail (labelled with BV421) (BioLegend – 133311; 5 µl per million cells) in staining buffer and washed twice ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend, Catalog: 344716) per 6 μL nanovial volume for 30 minutes in a 37°C incubator ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for >5 minutes at 4C with Fc Block (clone 93; BioLegend), then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend, #102427, 1:1000, 100), CD45-FITC (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... YAP1 was co-stained with cytokeratin 5 (CK5) or 14 (CK14) (BioLegend, San Diego, CA) and imaged with a Nikon fluorescence microscope (Melville ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were first blocked for 5 minutes using CD16/CD32 (Biolegend, San Diego, CA). Eosinophils were identified as CD11c-Siglec-F+ and neutrophils were identified as SiglecF-GR-1+ ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were stained with 2.5ug of anti-CD4-APC (RM4-5, Biolegend 100516) antibody in 1mL of sort buffer and incubated at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Physiology 2022Quote: ... and then stained for subsets of markers at 5 μg/ml: Ly6G-BV421(Biolegend, 127628), Ly6C-BV605 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Then resuspend and count in 1640 complete medium containing CD28 (5 μg/ml, 302934, Biolegend) and IL-2 (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... mice were administered 5 µg Alexa Fluor (AF) 488-labelled anti-CD45.2 antibody (BioLegend, US) intravenously (i.v. ...
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bioRxiv - Cancer Biology 2023Quote: ... and matched isotype controls APC mouse IgG2b k isotype control (5 μL/test; BioLegend #400322), APC Rat IgG2b ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were blocked using 5% goat serum and stained using antibodies against CD41 (133902, Biolegend) and laminin (L9393 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then stained overnight at 4C with anti-CD4 (AlexaFluor488, clone RM4-5, BioLegend) and anti-CD45.1 (Alexafluor647 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... spun for 5 minutes at 300 × g and washed once with 1x staining buffer (BioLegend). iNSCs were stained in suspension for 30 minutes at 37°C on an orbital shaker (500 rpm) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-CD11b (25-0112-81 ...
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bioRxiv - Immunology 2023Quote: ... we injected 5 μg of PE-Cy7 dye-conjugated anti-CD45 (Clone 30-F11, BioLegend) monoclonal antibodies (mAb ...
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bioRxiv - Cell Biology 2024Quote: ... human donor RGCs were pretreated with 5 mg/mL annexin V (BioLegend, San Diego, CA), a calcium-dependent membrane-bound protein with high affinity to PS ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified cells were plated at 1×105 cells per well on a 96 well plate coated with 10μg/ml αCD3 (Biolegend), and cultured with 2μg/ml soluble αCD28 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... and transferred to round-bottom 96-well plates for staining with Aqua live/dead stain (Life Technology or Biolegend), followed by Fc receptor blocking (normal immunoglobulin ...
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bioRxiv - Immunology 2022Quote: ... CAR T cells were incubated for 24 hours in flat bottom 96 well plates that were pre-coated overnight with 10 μg/mL of purified anti-CD244 (clone C1.7, BioLegend), CD2 (clone LT2 ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 1×106 splenocytes were isolated from mice and cultured on Immulon U-bottom plates coated with antibodies for Ly49D (0.5mg/ml) (Biolegend) or NKp46 (1mg/ml ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cell were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cells (106 cells/mL) were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD69-) from OT-I mice were stimulated with plate-bound anti-CD3 mAb (5µg/ml, clone 145-2C11, Biolegend), and soluble anti-CD28 (1µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... Cell cultures were stimulated for 3 days with either 100 ng/mL soluble or 1.25 μg/mL immobilised (plate-bound) anti-CD3 mAb (clone OKT3, BioLegend) in combination with 1 μg/mL soluble anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: Plates (48- or 96-well, flat bottom) were coated with 10 μg/ml αCD3ε mAb (BioLegend, San Diego, CA) and incubated at 37°C for 5 hours ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with FACS-wash (FW-1% FBS in PBS) in 96-well V-shape plates and stained with DAPI (0.1 µg/ml) (Biolegend) for 5 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... were added to the isolated lymphocyte suspension before seeding at 1mL/well in a 24-well plates coated with 1µg/mL of anti-CD3ε (100340, Biolegend) 24 h before the procedure.
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Immunology 2024Quote: ... cells were removed by washing and the plates were washed and probed with biotin α-IFNγ (clone XMG1.2, Biolegend) followed by incubation with Streptavidin-ALP (Mabtech) ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were seeded in 96-well plates and cultured alone or stimulated with anti-CD3 antibody (Clone: OKT3, Biolegend) or treated with 50 ng/ml IL-15 for 4 days ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... 1ug/ml) and plate bound anti-CD3 (500 ug/ml) in the presence of Brefeldin A (1:1000, Biolegend) for 4 hours ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...