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Citations for BioLegend :
1251 - 1300 of 8965 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Genomics 2023Quote: ... A subset of the hiPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2024Quote: ... anti-SHIP-1 (P1C1-A5, PE), human IgE (MHE-18, Alexa Fluor 647), HLA-DR (L243, APC-Cy7) (all from Biolegend); and phospho-p65 NF-κB (K10-895.12.50 ...
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
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bioRxiv - Cell Biology 2019Quote: ... 3 and 6 were stained with Calcein-AM (BIOLEGEND, 425201) and PI (SIGMA ...
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bioRxiv - Microbiology 2021Quote: ... and intracellularly stained with anti-IL-6 (PE-Cy7, BioLegend), and anti-CXCL10 (PE ...
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bioRxiv - Immunology 2022Quote: ... Permeabilized cells were intracellularly stained with Bcl-6 (Biolegend 358512). Cells were acquired on Attune NxT Flow Cytometer and analyzed with Attune NxT software v 4.2.0.
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bioRxiv - Cancer Biology 2022Quote: ... anti-H-2Kb/H- 2Db (clone 28-8-6, Biolegend), anti-CD86 (clone GL-1 ...
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bioRxiv - Immunology 2021Quote: ... 40ng/ml rmGM-CSF and 40ng/ml rmIL-6 (BioLegend), with or without 50% E.G7-OVA supernatant ...
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bioRxiv - Immunology 2022Quote: ... TH17 skew media contained IL-6 (20ng/mL; BioLegend, 575704), IL-23 (10ng/mL ...
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bioRxiv - Neuroscience 2023Quote: ... TH17 skew media contained IL-6 (20ng/mL; BioLegend, 575704), IL-23 (10ng/mL ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (4-6; BioLegend). The enriched samples were then stained with APC-labeled Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... 1) AnnexinV/7-AAD staining (Kit from Biolegend Cat# 640922): At the end of the EMT experiment described above ...
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bioRxiv - Cancer Biology 2022Quote: ... Zombie Green™ Fixable Viability Kit (1:1000, Biolegend 423111) or DAPI (1:10000 ...
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bioRxiv - Immunology 2023Quote: ... PE-coupled anti-KIT (1:100, clone 2B8, BioLegend, #105808), FITC-coupled anti-ST2 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Biophysics 2022Quote: ... Monoclonal antibodies against human Glycophorin AB (CD235ab) (HIR2; catalog number: 306602) and human CD47 (CC2C6; catalog number: 323102) were purchased from BioLegend.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat. #: 316904; anti-human BCMA conjugated to APC, BioLegend, Cat ...
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bioRxiv - Immunology 2023Quote: ... FITC anti-human CD18 antibody and FITC anti-human CD11c antibody (clone Bul15) were purchased from BioLegend (San Diego, USA). Rabbit anti-human MERTK antibody (D21F11 ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: Conditioned media samples were analyzed using commercially available ELISAs as per manufacturer guidelines (BioLegend, San Diego, CA), at 2X and 8X dilutions for IL-6 ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were normalised to protein concentration before being subject to an ELISA protocol or TNF-α (Biolegend), IL-12 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Collected supernatants were assessed for cytokine content using ELISA MAX™ Standard Set Mouse IFN-γ (Biolegend). DCs were analyzed for the expression of CD86 and MHC class II using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... with supernatant IFNγ level measured by enzyme-linked immunosorbent assay (ELISA) following manufacturer’s protocol (Biolegend, Cat# 430104).