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Citations for BioLegend :
1501 - 1550 of 8965 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Microbiology 2021Quote: ... malayi were individually incubated at 37 °C in RPMI 1640 with L-glutamine with 125 nM DRAQ5 (Biolegend) for 24-48 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Systems Biology 2020Quote: ... up to 1×106 EnSC/100 µL of Magnetic Bead Buffer (0.5% BSA in PBS) were combined with 5 µL/1×106 cells phycoerythrin (PE) conjugated anti-human SUSD2 antibody (BioLegend, London, UK). After 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Human pluripotent stem cell-derived cardiomyocytes used for IF-FISH were sorted by gating for SIRPA+ (PE-Cy7 anti-human CD172a/b, Biolegend, 1:200) and CD90- (APC anti-human CD90 (Thy1 ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (Biolegend, clone HI30), 1:100 diluted anti-human CD3-FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, 1/100), Sca-1 (Pacific Blue ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Pathology 2021Quote: ... were coated with purified GM-CSF capturing antibody from ELISA MAX™ Deluxe Set Mouse GM-CSF (Biolegend), left overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... TNFα and IL6 cytokines were determined in plasma and tissue lysates by ELISA (BioLegend, IL6, #431304; TNFα, #430904). Cytokine expression in tissue lysates are expressed as pg/mg protein concentration.
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Biochemistry 2023Quote: ... Cytokine release was measured in cell culture supernatants using ELISA per the manufacturer’s protocol with slight modifications (Biolegend). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and stained intracellularly with anti-BCL-6 Alexa647 (IG191E/A8; Biolegend). Non-human primate LN suspensions and PBMC were stained with the same protocol ...
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bioRxiv - Immunology 2020Quote: ... interleukin (IL)-6 (10 ng/mL, Biolegend, San Diego, CA, USA), IL-1β (10 ng/mL ...
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bioRxiv - Genomics 2022Quote: ... and 20 ng/mL of recombinant IL-6 (BioLegend, Cat# 575702) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and stained with Zombie Aqua Fixable Viability Kit (1:100; Biolegend) for 15 min at room temperature (RT ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human PVR/CD155 (Clone SKII.4, Biolegend), PE anti-human MICA (Clone 159227 ...
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bioRxiv - Cell Biology 2020Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
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bioRxiv - Immunology 2021Quote: ... BV421-conjugated mouse anti-human CD34 (Biolegend, CA, USA), PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Pacific Blue™ anti-human IgD (BioLegend, 348224). After 20 min ...
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bioRxiv - Immunology 2022Quote: PBMCs were firstly incubated with Human TruStain FcX (Biolegend) at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked using human TruStain FcX (BioLegend), and cells were then subsequently stained for surface markers anti-CD11c-FITC (Bu15 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-human PD1-APC (Clone EH1202H7, Biolegend) all diluted 1:100 ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human CD161-AF488 (Clone NKR-P1A, Biolegend), mouse anti-human IgD-BV421 (Clone IA6-2 ...
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bioRxiv - Immunology 2019Quote: ... The following anti-human antibodies were purchased from Biolegend, CD62L (DREG-56) ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human/mouse E-Cadherin-AlexaFluor 647 (147304; BioLegend) and DAPI for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... and anti-human CD326 (EPCAM)-AlexaFluor 647 (324212; BioLegend), anti-human/mouse E-Cadherin-AlexaFluor 647 (147304 ...
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bioRxiv - Neuroscience 2019Quote: ... iMGLs were stimulated with human BMP9 (20ng/ml; Biolegend), Pam3csk (100 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... and consisted of anti-human CD3 APC-Cy7 (BioLegend), anti-human CD4 Brilliant Violet 786 (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... using both human and mouse Fc blocking reagents (BioLegend). Cellular suspensions were loaded on a Chromium Single-Cell Instrument (10x Genomics ...
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bioRxiv - Immunology 2021Quote: ... Human TruStain FcX Fc receptor blocking reagent (BioLegend #422302) was used at a 1:100 dilution in PBS with 2.5 g bovine serum albumin (Sigma Aldrich #A3059 ...
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bioRxiv - Immunology 2021Quote: ... the cells were supplemented with Human TruStain FcX (Biolegend) to reduce non-specific antibody staining and 5uM Rhodium-103 (Fluidigm ...
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bioRxiv - Microbiology 2021Quote: ... PE/Cy5 anti-mouse/human CD44 (Biolegend, Cat# 103010), PE/Cy7 anti-mouse Ter119 (Biolegend ...