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Citations for BioLegend :
1251 - 1300 of 7713 citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were harvested 72 hours post transduction and HLA-C expression determined by staining with anti-HLA (W6/32) (Biolegend) antibody ...
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bioRxiv - Genomics 2021Quote: ... Sample from each donor is also stained with unique hash-tag barcoded antibody (Biolegend totalseq-C, cat# 394661, cat# 39466310). After two washes ...
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bioRxiv - Bioengineering 2020Quote: ... cells were recovered from wells and were incubated at 4°C for 30 minutes with antiCD3 antibody (Biolegend, CA, USA) and then were examined by flow cytometry.
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2021Quote: ... as determined by 2% agarose gel electrophoresis.49 The soluble chromatin fraction was incubated with either anti-c-Rel antibody or anti-IgG control antibody (Biolegend) coupled to Protein G beads (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... samples were thawed from −80°C and were stained for surface markers: Zombie UV fixable viability dye (Biolegend, Cat#423107), anti-CD3-BUV395 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed in 1X perm buffer for 20-30 min at 4°C using the following conjugated antibodies all purchased from BioLegend: mouse anti-IFN-γ (clone XMG1.2) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... and 30 minutes at 4°C with the following surface antibodies: CD8 PerCP-Cy5.5 (clone 53–6.7, Biolegend, cat#100734), CD44 BV650 (clone IM7 ...
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bioRxiv - Immunology 2022Quote: ... Samples sorted for 10x Genomics single-cell RNAseq were individually labelled with an oligonucleotide-linked hashing antibody (Totalseq-C, Biolegend) in addition to the staining cocktail and sorted into a single tube according to the gating strategy shown in Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Microbiology 2023Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Immunology 2023Quote: ... Murine cells were stained for 30 min at 4°C with the following antibodies (all from Biolegend, unless otherwise stated): CD3 APC(Clone:17A2 Cat:100236 ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were precooled for 20 min at 4 °C and then incubated with HDL-SOG for 40 min at 4 °C with 50 μg HDL per ml PBS or with anti-SCRB1 antibody-SOG (BioLegend, 363201 ...
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bioRxiv - Biochemistry 2023Quote: ... The NMM/DMSO was incubated for 40 hours at 4°C while the primary antibody anti-tubulin beta- III (#802001, BioLegend), diluted at 1:2000 in 0.15% Triton-X in PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated O/N at 4°C with a mouse anti-iNOS2 IgG antibody labeled with Alexa Fluor 564 (BioLegend)) or a mouse anti-ARG-1 IgG antibody labeled with FITC (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained for 20-30 min at 4°C with an antibody mix composed of: Zombie UV viability dye (BioLegend), anti-CD45RA (Beckman) ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Cancer Biology 2023Quote: ... The FCM panel used to analyze EMT phenotype of in vitro tumor cells relied on the use of the anti-mouse pancytokeratins (C-11; Biolegend), CD73 (TY/11.8 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... or cells cultured in vitro were subjected for surface marker staining with the following reagents for 20 min at 4 °C: Brilliant Violet 650TM-conjugated anti-mouse CD3 Ab (clone 17A2; BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse CD4 Ab (clone GK1.5 ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were treated with AL009 for 48 hours at 37° C and cell surface markers and cytokine production were analyzed via flow cytometry and LEGENDplex (Biolegend), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension from mouse esophagi was incubated at 37°C for 30 minutes with Integrin β1 antibody (PE anti-mouse/rat CD29; 102207; Biolegend) at 1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled stained in PBS for 20 mins at 4°C with a live dead marker (Zombie UVTM, Biolegend). Surface staining was performed in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... samples were incubated overnight at 4°C with combinations of the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), AF532-labeled iNOS (CXNFT ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Immunology 2023Quote: DCs were differentiated from bone marrow precursors obtained from naive BALB/c mice in the presence of murine GM-CSF (10ng/ml) and IL-4 (10ng/ml) both from Biolegend for 7 days ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody staining was carried out at 4°C overnight with the following antibody dilutions in blocking buffer: CD52-APC (clone HI186, BioLegend) 1:200 ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2024Quote: ... then cells were stained with monoclonal antibodies for 30 min at 4°C in Brilliant Stain Buffer (BioLegend, cat. #563794). See supplementary information for details on flow cytometry antibodies ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells isolated from a single organ in each mouse were individually incubated with unique TotalSeq-C anti-mouse antibodies from BioLegend. All CD4+ T cells were barcoded in the above manner to distinguish between different organs and mice before pooling cells together ...
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bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...