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Citations for BioLegend :
1201 - 1250 of 7713 citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2024Quote: ... cells were removed by washing and the plates were washed and probed with biotin α-IFNγ (clone XMG1.2, Biolegend) followed by incubation with Streptavidin-ALP (Mabtech) ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... were added to the isolated lymphocyte suspension before seeding at 1mL/well in a 24-well plates coated with 1µg/mL of anti-CD3ε (100340, Biolegend) 24 h before the procedure.
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... 1:100), GR1 (108428, 1:300), NK1.1 (108728, 1:200), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, IL-22 (12-7221-82 ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...
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bioRxiv - Immunology 2022Quote: ... and transcription factor staining kit (Biolegend). To assess the effect of RORγt inhibition on IL-17 production the specific inhibitor SR1001 (Tocris ...
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bioRxiv - Microbiology 2020Quote: ... Zombie UV Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Immunology 2023Quote: ... Zombie UV Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Annexin V Apoptosis Detection Kit (Biolegend) was used ...
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bioRxiv - Immunology 2023Quote: ... Zombie NIR Fixable Viability Kit (Biolegend). Samples were blocked with anti-CD16/32 (2.4G2 ...
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bioRxiv - Microbiology 2023Quote: ... Zombie Aqua Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... or AquaTM Fixable Viability Kit (BioLegend). Intracellular staining of immune cells was performed using the following Abs and a Foxp3 staining buffer kit (eBioscience ...
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bioRxiv - Cell Biology 2023Quote: ... Zombie NIR Fixable Viability Kit (BioLegend) staining was performed to exclude dead cells and debris from the analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Zombie Aqua Fixable Viability Kit (BioLegend), and the FOXP3/Transcription Factor Staining Kit (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... CD11b (AF594, 1:200), CD14 (PE-Cy5, 1:150), CD204 (PE-Cy7, 1:100) and Zombie NIR (1:1000) (Biolegend); CTY was captured on the PE channel.
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bioRxiv - Cell Biology 2021Quote: ... PAC-1 FITC (1:12, D Bioscience) and annexin V APC (1:60, Biolegend) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... TGM-1 (SigmaAldrich®, HPA040171, 1:150) and CD31 (BioLegend®, 303126, 1:100). Secondary antibodies were also applied overnight in a 3% BSA solution in PBS frequently alongside rhodamine-phalloidin and DAPI ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were transferred to new tubes and incubated overnight at 4°C with anti-GFP Nanobody Affinity gel (BioLegend). The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... DC were stained with PE CD45 (30-F11) and APC CD11c (N418) for 25 minutes at 4°C (all Biolegend). Excess antibodies were removed by washing with HBSS + 0.1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Amplified antibody barcodes were recovered from the supernatant and were processed to generate TotalSeq-C libraries as instructed by the manufacturers (10X Genomics and BioLegend, TotalSeq-C with 10x Feature Barcoding Protocol) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Genomics 2020Quote: ... loss of MHC class I expression in B2M knock-outs was assessed by staining with FITC-conjugated anti-HLA-A,B,C (W6/32, Biolegend). To maximize surface expression of IFNγ regulated proteins ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were fixed for 15 min at 37°C after surface marker and viability dye staining using Fixation Buffer (Biolegend), followed by permeabilization for 1h at −20°C with True-Phos™ Perm Buffer (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... Primary antibody labeling was performed in blocking buffer overnight at 4°C in a humidified chamber using the following antibodies: anti-CD4 Alexa Fluor 647 (BioLegend), goat anti-IgD purified (Cedarlane Labs) ...
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Systems Biology 2020Quote: ... The cells were incubated with each antibody for 45 min at −22–25°C after FcR blocking using Fc blocker (Human TruStain FcX, Biolegend) or anti-mouse CD16/CD32 antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with brefeldin A 2h at 37°C before surface stain with a mix of CD107a (LAMP1) [clone 1D4B (BioLegend)] and CD107b (Mac-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...