Labshake search
Citations for BioLegend :
1201 - 1250 of 2743 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... anti-human IL-6 (Biolegend), or isotype control antibodies for 30 min.
-
bioRxiv - Physiology 2023Quote: ... and IL-6 (BioLegend #431307) were measured by ELISA.
-
bioRxiv - Pathology 2023Quote: ... and IL-6 (Biolegend, 431304) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2024Quote: ... IL-6(Cat. #431301, Biolegend) and glucose(Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
-
bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
-
bioRxiv - Bioengineering 2024Quote: ... After 48h of incubation at 37 °C and 5% CO2 the co-cultures were prepared for flow cytometry analysis by staining for CD3 (cat. # 317318, Biolegend) and viability (DAPI ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained directly for determining NK cell numbers and Ki67 staining or incubated for 4 hours in medium containing Brefeldin A (Biolegend) and Monensin (Biolegend ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
-
bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
-
bioRxiv - Immunology 2021Quote: ... fixed with 4% PFA for 15 minutes and subsequently stained for 30 minutes with anti-CD4-APC and anti-CD8-BV605 (Biolegend) in FACS buffer ...
-
bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
-
bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
-
bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the media-trypsin mix was aspirated and the cells were fixed via incubation in 50 µL of 4% formaldehyde (BioLegend) for 20 minutes at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... and then cells were fixed with 4% PFA at room temperature for 10 minutes and permeabilized with intracellular staining perm wash buffer (BioLegend), and stained with the appropriate intracellular antibodies ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
-
bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
-
bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
-
bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
-
bioRxiv - Immunology 2023Quote: ... CAR-T cells were cocultured with NALM6GL cells at indicate E/T ratio for 4 hours with the presence of 1x Brefeldin A (Biolegend). Cells were first stained with the Live/Dead-NearIR dye before fixed ...
-
bioRxiv - Cell Biology 2023Quote: ... at the indicated concentrations for 4 hours in 100 µL of RPMI 1640 medium containing 0.2% BSA with Brefeldin A Solution (BioLegend, 420601). After treatment ...
-
bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
-
bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
-
bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... Efficiency of CD4+ T cell depletion was routinely assessed in small amounts of peripheral blood on d0 prior to LCMV challenge by flow cytometry staining with a non-competitively binding anti-CD4 antibody (clone RM4-4, Biolegend).
-
bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
-
bioRxiv - Immunology 2023Quote: ... cells were fixed with 4% PFA for 10 min at RT followed by permeabilization with the True-Phos Perm buffer (Biolegend) for 20 min on ice ...
-
bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
-
bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
-
bioRxiv - Immunology 2022Quote: ... or spleen were resuspended in MACS buffer and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD4 (clone RM4-5) ...
-
bioRxiv - Microbiology 2022Quote: ... They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4) and either anti-Ki-67-PE (BioLegend) or anti-γ-H2AX-PE (clone 2F3 ...
-
bioRxiv - Immunology 2024Quote: ... single cells from the omentum tumor were incubated for 4 hours with Cell Activation Cocktail (with Brefeldin A) (catalog no. 423303, Biolegend) prior to staining.
-
bioRxiv - Immunology 2024Quote: Mitochondrial mass was determined in DCs by fixing the cells with PFA 4% and labeling them with the probe MitoSpy Green FM (Biolegend). Green fluorescence was analyzed by flow cytometry (FACScan ...
-
bioRxiv - Neuroscience 2024Quote: ... During the last 4 days of culture the microglial maturation was promoted by adding 100 ng/mL human CD200 (#77002, Biolegend) and 100 ng/mL human CX3CL1 (#300-31 ...
-
bioRxiv - Immunology 2024Quote: DCs were differentiated from bone marrow precursors obtained from naive BALB/c mice in the presence of murine GM-CSF (10 ng/ml) and IL-4 (10 ng/ml) both from Biolegend for 7 days ...
-
bioRxiv - Neuroscience 2024Quote: ... the media were again changed to serum starved media with the addition of human recombinant cytokines (10ng/ml TNFα, BioLegend #570102, 10ng/ml IL-17, Proteintech #Hz-1113, 10ng/ml IL-4, BioLegend #574004 ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
-
bioRxiv - Microbiology 2024Quote: ... IL-10, IL-12p70, IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFN-γ, TNF-α, IP-10 and GM-CSF; BioLegend) assay was performed on patient plasma samples as per the manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... Ultra-LEAFTM purified anti-human IL-10 mAb (JES3-9D7) and 7-Aminoactinomycin D (7-AAD) were purchased from BioLegend (San Diego, CA, USA). Anti-IgA mAb (mA-6E1 ...
-
bioRxiv - Microbiology 2020Quote: ... C (Biolegend) and 10 μg/mL anti HLA-DR ...
-
bioRxiv - Bioengineering 2021Quote: ... and PE/Cyanine 7 anti-human CD4 antibody (#317414, Biolegend), APC/Cyanine 7 anti-human CD8 antibody (#344714 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1) AnnexinV/7-AAD staining (Kit from Biolegend Cat# 640922): At the end of the EMT experiment described above ...
-
bioRxiv - Bioengineering 2022Quote: ... and assayed with Annexin V/7-AAD staining kit (BioLegend). The cells were stained with Annexin V diluted in 1x Annexin V buffer for 15 minutes and incubated with 2x 7-AAD for 5 minutes ...