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Citations for BioLegend :
1051 - 1100 of 8451 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend, #317304) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Immunology 2024Quote: ... The wells were then washed twice with PBS + 2% FBS and then stained with anti-human CD14 (Biolegend 301818 clone M5E2) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... T cells were cultured in a 96-well round bottom plate at the density of 50,000 cells/200 µL with 100 U/mL IL-2 (BioLegend, Cat. 589104) for 96 hours ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured in a 96-well round bottom plate at a density of 50,000-600,000 cells/300 µL with 100 U/mL IL-2 (Biolegend, Cat. 575404 (mouse) 589104 (human)) ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... The cells were re-suspended in PBS / 2% HI-FBS / 10mM EDTA-Na (pH 8.0) and stained with PE/Cyanine7 anti-mouse CD45 (BioLegend, #103114, RRID:AB_312979) and FITC anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were treated with mechanosensing and transduction inhibitors using an antibody against integrin β1 (2 µg/mL; anti-integrin β1; Biolegend, USA) and chemical inhibitors against PI3K (1 µM ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm Staining Buffer (SB; Biolegend #420201). Cells were washed twice at 300 g for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm staining buffer (SB; Biolegend #420201) or media buffer (Thermo Fisher Scientific DMEM #12634010 or RPMI #61870010 ...
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bioRxiv - Immunology 2021Quote: ... prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most) or Novus Biologicals (MARCO ...
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bioRxiv - Genomics 2021Quote: ... This was followed by 30min incubation at 4 °C with a Streptavidin-PE (Biolegend, 1:5000), anti c-kit-APC (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were incubated in 3% BSA for 1 hr before being treated overnight at 4°C with the respective primary antibodies : PAX6 for ectoderm (1:300; BioLegend, PRB-278P), Brachyury for mesoderm (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 washes were performed prior to secondary antibody staining with anti-rabbit bv421 1:500 (BioLegend 406410) with 3 additional washes after secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... CD11a (M17/4, Biolegend), CD27 (LG-7F9) ...
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bioRxiv - Immunology 2021Quote: ... and IL-4 (BioLegend) were prepared in PBS at concentrations of 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD11a (M17/4; Biolegend), and CD69 (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (BioLegend, 574004), IL-13 (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained for 30min in flow buffer (PBS, 1mM EDTA, 2% FBS) with antibodies against CD8α-FITC (53-6.7; Biolegend unless otherwise noted), CD19-PerCP/Cy5.5 (1D3) ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... EBs were plated on Geltrex™-coated 6-well plates (10 EBs/well) in APEL™2 medium supplemented with VEGF (40 ng/ml) (BioLegend), SCF (50 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... AML cell lines and PBMCs from healthy individuals and AML patients were stained with HERV-K102 Env-TM autoantibody and the secondary antibody PE F(ab’)2 Goat anti-human IgG Fcγ (BioLegend, Cat 398004), as well as PE Mouse IgG1 ...
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bioRxiv - Neuroscience 2024Quote: ... CTPI-2 toxicity was assessed by treating assembloids with 50uM CTPI-2 for 24hours prior to dissociation and staining with an Annexin V Cell Viability assay (Biolegend #422201 & #640935) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated at 4°C overnight in primary antibodies against anti-HA (1:100; BioLegend; 901533), CA3 (1:150 ...