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Citations for BioLegend :
851 - 900 of 8451 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... eleven different TotalSeq™-C anti-human Hashtag oligo-antibodies (0.5 g/106cells; BioLegend, Supplementary table 2) were added to the PBMCs stimulated with myelin peptides to enable further multiplexing of the sorted samples at the single-cell TCR sequencing step ...
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bioRxiv - Immunology 2022Quote: ... tissue sections were blocked with 3% BSA and Human TruStain FcX (1:100, BioLegend) in PBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 3% paraformaldehyde and stained with anti-LFA-1 (BioLegend, 301202) and phalloidin-AF 488 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Immunology 2024Quote: ... unless otherwise specified: αCD40 mAb (Invitrogen or BioLegend, Clone HM40-3, 1 μg/mL), IL-4 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... rmIL-3 (Biolegend) was added to media (1 ng/ml) ...
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bioRxiv - Cell Biology 2024Quote: ... incubated overnight at 4°C with primary antibodies (anti-HA, 1:1000, BioLegend, #901502 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice and resuspended in 1:4 fixation buffer (BioLegend 420801) in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...
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bioRxiv - Developmental Biology 2020Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
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bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
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bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 2% PFA for 20min and permeabilized with Perm/Wash buffer (Biolegend, Cat No.421002) for 30mins ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were then removed with a 2-minute incubation in RBC Lysis Buffer (10X) (BioLegend 420301). The solution was centrifuged again at 300 x g ...
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bioRxiv - Immunology 2024Quote: ... 0.25 x 106 cells were stimulated with either plate-bound 11-CD3 (BioLegend; RRID: AB_11149115; 2 µg/mL) and plate-bound 11-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... The culture medium was supplemented with 10 ng/mL IL-2 and 25 ng/mL IL-15 (Biolegend). On the next day ...
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bioRxiv - Immunology 2024Quote: ... The pooled sample was then stained with diluted TotalSeq-C cell surface marker antibodies (BioLegend, Supplementary Table 2) in PBS containing 2% FBS for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Cell Biology 2021Quote: ... were taken on day 1 and 3 and analyzed for TGFβ using ELISA assay (BioLegend) and IFNγ and TNFα using a multiplex-bead based assay (Meso Scale Discovery ...
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bioRxiv - Immunology 2021Quote: ... and for 3 h at 37°C with Brefaldin A (10 μg mL-1; Biolegend). Cell surfaces were stained and cells fixed as described above ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-SSEA-3 rat IgM antibody (1:1000, BioLegend, 330302) at 4°C for 1 h following incubation with fluorescein (FITC ...
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bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated at +4°C with the 4G8 (Biolegend 800706, 1/500), Iba1 (Wako 1919741 ...
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bioRxiv - Systems Biology 2022Quote: ... Vimentin was performed overnight at 4°C using anti-HA (1:750, BioLegend #901502), anti-SLC1A5 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Anti-LFA-1 antibody (M17/4) was purchased from BioLegend (San Diego, CA, USA). Fluorescence-conjugated CD3e ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher], rabbit polyclonal anti-tubulin β-3 IgG at 1:500 [Biolegend]) in PBS containing 1% Triton X-100 for 48 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Extracellular staining was performed in FACS buffer (PBS + 2% FBS + 2mM EDTA) with the following antibodies: CD4 (GK1.5, Biolegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 µg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...